Expression of mouse igf2 mRNA-binding protein 3 and its implications for the developing central nervous system

Hiroshi Mori, Shin ichi Sakakibara, Takao Imai, Yuki Nakamura, Takatoshi Iijima, Akira Suzuki, Yoshihiro Yuasa, Masatoshi Takeda, Hideyuki Okano

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Functional analyses of neural RNA-binding proteins have focused mainly on their roles as modulators of posttranscriptional gene regulation, e.g., alternative splicing, dendritic mRNA localization, and local translation. Here we identified a mouse homologue of human IMP3, which is known to bind to and repress the translation of igf2 leader 3 mRNA. The mouse igf2 mRNA-binding protein 3 (mIMP3) is a member of the zipcode binding protein-1 (ZBP-1) family previously reported in chick fibroblast cells. mIMP3 was expressed in undifferentiated neuroepithelial cells and some postmitotic neurons at early embryonic stages (E10.5-E12.5), and its expression level decreased after the midembryonic stage (E12.5) until birth. The expression profile of mIMP3 is very similar to that of mouse igf2 leader 3 mRNA. In vitro UV cross-linking experiments showed that mIMP3 preferentially bound to igf2 leader 3 mRNA rather than igf2 leader 4 mRNA and did not bind the zipcode region of β-actin or c-myc mRNA. Furthermore, persistent expression of mIMP3 protein in an undifferentiated P19 cell line revealed that mIMP3 inhibited neuronal differentiation morphologically and immunohistochemically. Taken together, these observations raise the possibility that mIMP3 represses neuronal differentiation through the regulation of igf2 mRNA expression.

Original languageEnglish
Pages (from-to)132-143
Number of pages12
JournalJournal of Neuroscience Research
Volume64
Issue number2
DOIs
Publication statusPublished - 2001 Apr 15

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Carrier Proteins
Central Nervous System
Messenger RNA
Neuroepithelial Cells
RNA-Binding Proteins
Alternative Splicing
Actins
Fibroblasts
Parturition
Neurons
Cell Line

Keywords

  • IGF II
  • Neuronal differentiation
  • Posttranscriptional regulation
  • RNA-binding protein

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Expression of mouse igf2 mRNA-binding protein 3 and its implications for the developing central nervous system. / Mori, Hiroshi; Sakakibara, Shin ichi; Imai, Takao; Nakamura, Yuki; Iijima, Takatoshi; Suzuki, Akira; Yuasa, Yoshihiro; Takeda, Masatoshi; Okano, Hideyuki.

In: Journal of Neuroscience Research, Vol. 64, No. 2, 15.04.2001, p. 132-143.

Research output: Contribution to journalArticle

Mori, H, Sakakibara, SI, Imai, T, Nakamura, Y, Iijima, T, Suzuki, A, Yuasa, Y, Takeda, M & Okano, H 2001, 'Expression of mouse igf2 mRNA-binding protein 3 and its implications for the developing central nervous system', Journal of Neuroscience Research, vol. 64, no. 2, pp. 132-143. https://doi.org/10.1002/jnr.1060
Mori, Hiroshi ; Sakakibara, Shin ichi ; Imai, Takao ; Nakamura, Yuki ; Iijima, Takatoshi ; Suzuki, Akira ; Yuasa, Yoshihiro ; Takeda, Masatoshi ; Okano, Hideyuki. / Expression of mouse igf2 mRNA-binding protein 3 and its implications for the developing central nervous system. In: Journal of Neuroscience Research. 2001 ; Vol. 64, No. 2. pp. 132-143.
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