Expression of N-cadherin and α-catenin in astrocytomas and glioblastomas

N. Shinoura, N. E. Paradies, R. E. Warnick, H. Chen, J. J. Larson, J. J. Tew, M. Simon, R. A. Lynch, Yae Kanai, S. Hirohashi, J. J. Hemperly, A. G. Menoni, R. Brackenbury

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

We examined levels of mRNA and protein for N-cadherin, the predominant cadherin in neural tissues, and mRNA levels for the cadherin-associated protein, alpha-catenin, in a series of gliomas and in glioblastoma cell lines. mRNA levels for N-cadherin and alpha-catenin were significantly higher in glioblastomas than in low-grade astrocytomas or normal brain, while the levels of intact N-cadherin protein were similar in glioblastomas, low-grade astrocytomas and brain. In addition, there was no consistent relationship between invasiveness and expression of N-cadherin and alpha-catenin in highly invasive vs minimally invasive tumours within the same histopathological grade. To assess further the relationship between cadherin expression and neural tumour invasion, we measured N-cadherin expression, calcium-dependent cell adhesion and motility of several glioblastoma cell lines. While all N-cadherin-expressing lines were adhesive, no correlation was seen between the level of N-cadherin expression and cell motility. Together, these findings imply that, in contrast to the role played by E-cadherin in carcinomas, N-cadherin does not restrict the invasion of glioblastomas.

Original languageEnglish
Pages (from-to)627-633
Number of pages7
JournalBritish Journal of Cancer
Volume72
Issue number3
DOIs
Publication statusPublished - 1995
Externally publishedYes

Fingerprint

Catenins
Astrocytoma
Cadherins
Glioblastoma
alpha Catenin
Messenger RNA
Cell Movement
Cell Line
Proteins
Brain
Cell Adhesion
Glioma
Adhesives
Neoplasms

Keywords

  • Brain tumours
  • Cell adhesion
  • Cell motility
  • Invasion

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Shinoura, N., Paradies, N. E., Warnick, R. E., Chen, H., Larson, J. J., Tew, J. J., ... Brackenbury, R. (1995). Expression of N-cadherin and α-catenin in astrocytomas and glioblastomas. British Journal of Cancer, 72(3), 627-633. https://doi.org/10.1038/bjc.1995.384

Expression of N-cadherin and α-catenin in astrocytomas and glioblastomas. / Shinoura, N.; Paradies, N. E.; Warnick, R. E.; Chen, H.; Larson, J. J.; Tew, J. J.; Simon, M.; Lynch, R. A.; Kanai, Yae; Hirohashi, S.; Hemperly, J. J.; Menoni, A. G.; Brackenbury, R.

In: British Journal of Cancer, Vol. 72, No. 3, 1995, p. 627-633.

Research output: Contribution to journalArticle

Shinoura, N, Paradies, NE, Warnick, RE, Chen, H, Larson, JJ, Tew, JJ, Simon, M, Lynch, RA, Kanai, Y, Hirohashi, S, Hemperly, JJ, Menoni, AG & Brackenbury, R 1995, 'Expression of N-cadherin and α-catenin in astrocytomas and glioblastomas', British Journal of Cancer, vol. 72, no. 3, pp. 627-633. https://doi.org/10.1038/bjc.1995.384
Shinoura N, Paradies NE, Warnick RE, Chen H, Larson JJ, Tew JJ et al. Expression of N-cadherin and α-catenin in astrocytomas and glioblastomas. British Journal of Cancer. 1995;72(3):627-633. https://doi.org/10.1038/bjc.1995.384
Shinoura, N. ; Paradies, N. E. ; Warnick, R. E. ; Chen, H. ; Larson, J. J. ; Tew, J. J. ; Simon, M. ; Lynch, R. A. ; Kanai, Yae ; Hirohashi, S. ; Hemperly, J. J. ; Menoni, A. G. ; Brackenbury, R. / Expression of N-cadherin and α-catenin in astrocytomas and glioblastomas. In: British Journal of Cancer. 1995 ; Vol. 72, No. 3. pp. 627-633.
@article{e86c86626f734411a5896cce57dcbd68,
title = "Expression of N-cadherin and α-catenin in astrocytomas and glioblastomas",
abstract = "We examined levels of mRNA and protein for N-cadherin, the predominant cadherin in neural tissues, and mRNA levels for the cadherin-associated protein, alpha-catenin, in a series of gliomas and in glioblastoma cell lines. mRNA levels for N-cadherin and alpha-catenin were significantly higher in glioblastomas than in low-grade astrocytomas or normal brain, while the levels of intact N-cadherin protein were similar in glioblastomas, low-grade astrocytomas and brain. In addition, there was no consistent relationship between invasiveness and expression of N-cadherin and alpha-catenin in highly invasive vs minimally invasive tumours within the same histopathological grade. To assess further the relationship between cadherin expression and neural tumour invasion, we measured N-cadherin expression, calcium-dependent cell adhesion and motility of several glioblastoma cell lines. While all N-cadherin-expressing lines were adhesive, no correlation was seen between the level of N-cadherin expression and cell motility. Together, these findings imply that, in contrast to the role played by E-cadherin in carcinomas, N-cadherin does not restrict the invasion of glioblastomas.",
keywords = "Brain tumours, Cell adhesion, Cell motility, Invasion",
author = "N. Shinoura and Paradies, {N. E.} and Warnick, {R. E.} and H. Chen and Larson, {J. J.} and Tew, {J. J.} and M. Simon and Lynch, {R. A.} and Yae Kanai and S. Hirohashi and Hemperly, {J. J.} and Menoni, {A. G.} and R. Brackenbury",
year = "1995",
doi = "10.1038/bjc.1995.384",
language = "English",
volume = "72",
pages = "627--633",
journal = "British Journal of Cancer",
issn = "0007-0920",
publisher = "Nature Publishing Group",
number = "3",

}

TY - JOUR

T1 - Expression of N-cadherin and α-catenin in astrocytomas and glioblastomas

AU - Shinoura, N.

AU - Paradies, N. E.

AU - Warnick, R. E.

AU - Chen, H.

AU - Larson, J. J.

AU - Tew, J. J.

AU - Simon, M.

AU - Lynch, R. A.

AU - Kanai, Yae

AU - Hirohashi, S.

AU - Hemperly, J. J.

AU - Menoni, A. G.

AU - Brackenbury, R.

PY - 1995

Y1 - 1995

N2 - We examined levels of mRNA and protein for N-cadherin, the predominant cadherin in neural tissues, and mRNA levels for the cadherin-associated protein, alpha-catenin, in a series of gliomas and in glioblastoma cell lines. mRNA levels for N-cadherin and alpha-catenin were significantly higher in glioblastomas than in low-grade astrocytomas or normal brain, while the levels of intact N-cadherin protein were similar in glioblastomas, low-grade astrocytomas and brain. In addition, there was no consistent relationship between invasiveness and expression of N-cadherin and alpha-catenin in highly invasive vs minimally invasive tumours within the same histopathological grade. To assess further the relationship between cadherin expression and neural tumour invasion, we measured N-cadherin expression, calcium-dependent cell adhesion and motility of several glioblastoma cell lines. While all N-cadherin-expressing lines were adhesive, no correlation was seen between the level of N-cadherin expression and cell motility. Together, these findings imply that, in contrast to the role played by E-cadherin in carcinomas, N-cadherin does not restrict the invasion of glioblastomas.

AB - We examined levels of mRNA and protein for N-cadherin, the predominant cadherin in neural tissues, and mRNA levels for the cadherin-associated protein, alpha-catenin, in a series of gliomas and in glioblastoma cell lines. mRNA levels for N-cadherin and alpha-catenin were significantly higher in glioblastomas than in low-grade astrocytomas or normal brain, while the levels of intact N-cadherin protein were similar in glioblastomas, low-grade astrocytomas and brain. In addition, there was no consistent relationship between invasiveness and expression of N-cadherin and alpha-catenin in highly invasive vs minimally invasive tumours within the same histopathological grade. To assess further the relationship between cadherin expression and neural tumour invasion, we measured N-cadherin expression, calcium-dependent cell adhesion and motility of several glioblastoma cell lines. While all N-cadherin-expressing lines were adhesive, no correlation was seen between the level of N-cadherin expression and cell motility. Together, these findings imply that, in contrast to the role played by E-cadherin in carcinomas, N-cadherin does not restrict the invasion of glioblastomas.

KW - Brain tumours

KW - Cell adhesion

KW - Cell motility

KW - Invasion

UR - http://www.scopus.com/inward/record.url?scp=0028984908&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028984908&partnerID=8YFLogxK

U2 - 10.1038/bjc.1995.384

DO - 10.1038/bjc.1995.384

M3 - Article

VL - 72

SP - 627

EP - 633

JO - British Journal of Cancer

JF - British Journal of Cancer

SN - 0007-0920

IS - 3

ER -