Abstract
We examined levels of mRNA and protein for N-cadherin, the predominant cadherin in neural tissues, and mRNA levels for the cadherin-associated protein, alpha-catenin, in a series of gliomas and in glioblastoma cell lines. mRNA levels for N-cadherin and alpha-catenin were significantly higher in glioblastomas than in low-grade astrocytomas or normal brain, while the levels of intact N-cadherin protein were similar in glioblastomas, low-grade astrocytomas and brain. In addition, there was no consistent relationship between invasiveness and expression of N-cadherin and alpha-catenin in highly invasive vs minimally invasive tumours within the same histopathological grade. To assess further the relationship between cadherin expression and neural tumour invasion, we measured N-cadherin expression, calcium-dependent cell adhesion and motility of several glioblastoma cell lines. While all N-cadherin-expressing lines were adhesive, no correlation was seen between the level of N-cadherin expression and cell motility. Together, these findings imply that, in contrast to the role played by E-cadherin in carcinomas, N-cadherin does not restrict the invasion of glioblastomas.
| Original language | English |
|---|---|
| Pages (from-to) | 627-633 |
| Number of pages | 7 |
| Journal | British Journal of Cancer |
| Volume | 72 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 1995 |
| Externally published | Yes |
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Keywords
- Brain tumours
- Cell adhesion
- Cell motility
- Invasion
ASJC Scopus subject areas
- Oncology
- Cancer Research
Cite this
Expression of N-cadherin and α-catenin in astrocytomas and glioblastomas. / Shinoura, N.; Paradies, N. E.; Warnick, R. E.; Chen, H.; Larson, J. J.; Tew, J. J.; Simon, M.; Lynch, R. A.; Kanai, Yae; Hirohashi, S.; Hemperly, J. J.; Menoni, A. G.; Brackenbury, R.
In: British Journal of Cancer, Vol. 72, No. 3, 1995, p. 627-633.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression of N-cadherin and α-catenin in astrocytomas and glioblastomas
AU - Shinoura, N.
AU - Paradies, N. E.
AU - Warnick, R. E.
AU - Chen, H.
AU - Larson, J. J.
AU - Tew, J. J.
AU - Simon, M.
AU - Lynch, R. A.
AU - Kanai, Yae
AU - Hirohashi, S.
AU - Hemperly, J. J.
AU - Menoni, A. G.
AU - Brackenbury, R.
PY - 1995
Y1 - 1995
N2 - We examined levels of mRNA and protein for N-cadherin, the predominant cadherin in neural tissues, and mRNA levels for the cadherin-associated protein, alpha-catenin, in a series of gliomas and in glioblastoma cell lines. mRNA levels for N-cadherin and alpha-catenin were significantly higher in glioblastomas than in low-grade astrocytomas or normal brain, while the levels of intact N-cadherin protein were similar in glioblastomas, low-grade astrocytomas and brain. In addition, there was no consistent relationship between invasiveness and expression of N-cadherin and alpha-catenin in highly invasive vs minimally invasive tumours within the same histopathological grade. To assess further the relationship between cadherin expression and neural tumour invasion, we measured N-cadherin expression, calcium-dependent cell adhesion and motility of several glioblastoma cell lines. While all N-cadherin-expressing lines were adhesive, no correlation was seen between the level of N-cadherin expression and cell motility. Together, these findings imply that, in contrast to the role played by E-cadherin in carcinomas, N-cadherin does not restrict the invasion of glioblastomas.
AB - We examined levels of mRNA and protein for N-cadherin, the predominant cadherin in neural tissues, and mRNA levels for the cadherin-associated protein, alpha-catenin, in a series of gliomas and in glioblastoma cell lines. mRNA levels for N-cadherin and alpha-catenin were significantly higher in glioblastomas than in low-grade astrocytomas or normal brain, while the levels of intact N-cadherin protein were similar in glioblastomas, low-grade astrocytomas and brain. In addition, there was no consistent relationship between invasiveness and expression of N-cadherin and alpha-catenin in highly invasive vs minimally invasive tumours within the same histopathological grade. To assess further the relationship between cadherin expression and neural tumour invasion, we measured N-cadherin expression, calcium-dependent cell adhesion and motility of several glioblastoma cell lines. While all N-cadherin-expressing lines were adhesive, no correlation was seen between the level of N-cadherin expression and cell motility. Together, these findings imply that, in contrast to the role played by E-cadherin in carcinomas, N-cadherin does not restrict the invasion of glioblastomas.
KW - Brain tumours
KW - Cell adhesion
KW - Cell motility
KW - Invasion
UR - http://www.scopus.com/inward/record.url?scp=0028984908&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028984908&partnerID=8YFLogxK
U2 - 10.1038/bjc.1995.384
DO - 10.1038/bjc.1995.384
M3 - Article
C2 - 7669572
AN - SCOPUS:0028984908
VL - 72
SP - 627
EP - 633
JO - British Journal of Cancer
JF - British Journal of Cancer
SN - 0007-0920
IS - 3
ER -