Expression of natriuretic peptide system during embryonic stem cell vasculogenesis

Kentaro Doi, Hiroshi Itoh, Osamu Nakagawa, Toshio Igaki, Jun Yamashita, Tae Hwa Chun, Mayumi Inoue, Ken Masatsugu, Kazuwa Nakao

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The natriuretic peptide family consists of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), and C-type natriuretic peptide (CNP). We have elucidated that CNP is synthesized by endothelial cells. We have also shown that CNP secretion is potently suppressed by vascular endothelial growth factor (VEGF). In the present study. we examined the developmental gene expression of the natriuretic peptide system with the expression of VEGF and endothelial cell-specific receptor tyrosine kinases (RTKs), which expression is necessary for vasculogenesis, using embryoid bodies (EB) as an in vitro model for vascular development. When mouse embryonic stem (ES) cells were cultured in suspension culture. ES cells spontaneously differentiated into EB on day 4 and then into cystic EB (day 10). The VEGF gene transcript was detected early, on day 4. The expression of Flk-1, and flt-1 (the two VEGF receptors) and also of tie-2, which is crucial for blood island formation, was detected as early as day 4, and also on days 8 and 21. In contrast, the expression of flt-4, the receptor for VEGF-C, and tie-1, was first detected on day 21. Along with the developmental expression of these markers of differentiation for endothelial cells, the gene expression of CNP and its specific receptor, ANP-B receptor, was detected on days 4, 8, and 21. In contrast, the gene expression of BNP, which acts as a cardiac hormone, and the gene expression of the ANP-A receptor, which is specific to ANP and BNP, was first detected on days 8 and 21, respectively. These results indicate the distinct role of CNP in the natriuretic peptide family and the close linkage of CNP expression and endothelial cell differentiation, suggesting a possible role of CNP in vasculogenesis.

Original languageEnglish
Pages (from-to)18-22
Number of pages5
JournalHeart and Vessels
Volume12
Issue numberSUPPL. 12
Publication statusPublished - 1997
Externally publishedYes

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C-Type Natriuretic Peptide
Natriuretic Peptides
Embryonic Stem Cells
Embryoid Bodies
Brain Natriuretic Peptide
Endothelial Cells
Atrial Natriuretic Factor Receptors
Vascular Endothelial Growth Factor A
Gene Expression
Atrial Natriuretic Factor
Vascular Endothelial Growth Factor Receptor-3
Vascular Endothelial Growth Factor C
Vascular Endothelial Growth Factor Receptor-1
Developmental Genes
Differentiation Antigens
Receptor Protein-Tyrosine Kinases
Blood Vessels
Cell Differentiation
Suspensions
Hormones

Keywords

  • ES cells
  • Natriuretic peptides
  • Receptor tyrosine kinases
  • Vasculogenesis
  • VEGF

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Doi, K., Itoh, H., Nakagawa, O., Igaki, T., Yamashita, J., Chun, T. H., ... Nakao, K. (1997). Expression of natriuretic peptide system during embryonic stem cell vasculogenesis. Heart and Vessels, 12(SUPPL. 12), 18-22.

Expression of natriuretic peptide system during embryonic stem cell vasculogenesis. / Doi, Kentaro; Itoh, Hiroshi; Nakagawa, Osamu; Igaki, Toshio; Yamashita, Jun; Chun, Tae Hwa; Inoue, Mayumi; Masatsugu, Ken; Nakao, Kazuwa.

In: Heart and Vessels, Vol. 12, No. SUPPL. 12, 1997, p. 18-22.

Research output: Contribution to journalArticle

Doi, K, Itoh, H, Nakagawa, O, Igaki, T, Yamashita, J, Chun, TH, Inoue, M, Masatsugu, K & Nakao, K 1997, 'Expression of natriuretic peptide system during embryonic stem cell vasculogenesis', Heart and Vessels, vol. 12, no. SUPPL. 12, pp. 18-22.
Doi K, Itoh H, Nakagawa O, Igaki T, Yamashita J, Chun TH et al. Expression of natriuretic peptide system during embryonic stem cell vasculogenesis. Heart and Vessels. 1997;12(SUPPL. 12):18-22.
Doi, Kentaro ; Itoh, Hiroshi ; Nakagawa, Osamu ; Igaki, Toshio ; Yamashita, Jun ; Chun, Tae Hwa ; Inoue, Mayumi ; Masatsugu, Ken ; Nakao, Kazuwa. / Expression of natriuretic peptide system during embryonic stem cell vasculogenesis. In: Heart and Vessels. 1997 ; Vol. 12, No. SUPPL. 12. pp. 18-22.
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