Expression of Nephrin homologue in the freshwater planarian, Dugesia japonica

Tomomi Nakamura, Sota Takagi, Midori Matsumoto, Fumio Tashiro, Tatsuo Sakai, Koichiro Ichimura

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Excretory organs contain epithelial cells that form a filtration membrane specialized for ultrafiltration to produce primary urine. In vertebrates, the filtration membrane is made up of slit diaphragm (SD) formed by glomerular podocytes. Basal metazoans such as flatworms are also known have filtration epithelial cells, called flame cells, which exhibit SD-like structures. The molecular components of podocyte SD have been studied in detail, while those of the SD-like structures in basal metazoans including flatworms remain to be clarified. To determine whether the SD-like structures in flatworms have molecular components common to the SD in vertebrate podocytes, we examined the expression of gene homologue for mammalian nephrin, which encodes an essential transmembrane protein that participates in the formation of the SD, in a species of flatworms, planarian (Dugesia japonica). Flame cells were distributed throughout the entire body of the planarian, but the nephrin-expressing cells identified by in situ hybridization were mainly detected at body periphery excluding head region. The distribution pattern of nephrin-expressing cells was similar to that of proliferating cell nuclear antigen-expressing neoblasts, which are pluripotent stem cells characteristic to planarians. These findings indicated that the SD-like structures can be formed without the Nephrin protein in planarian flame cells.

Original languageEnglish
Pages (from-to)303-310
Number of pages8
JournalActa Histochemica et Cytochemica
Volume47
Issue number6
DOIs
Publication statusPublished - 2014

Fingerprint

Planarians
Diaphragms
Diaphragm
Fresh Water
Platyhelminths
Podocytes
Vertebrates
Epithelial Cells
Membranes
Pluripotent Stem Cells
nephrin
Proliferating Cell Nuclear Antigen
Ultrafiltration
Stem cells
In Situ Hybridization
Proteins
Genes
Head
Urine
Gene Expression

Keywords

  • Flame cell
  • Nephrin
  • Planarian
  • Protonephridium
  • Slit diaphragm

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Physiology
  • Pathology and Forensic Medicine
  • Histology

Cite this

Expression of Nephrin homologue in the freshwater planarian, Dugesia japonica. / Nakamura, Tomomi; Takagi, Sota; Matsumoto, Midori; Tashiro, Fumio; Sakai, Tatsuo; Ichimura, Koichiro.

In: Acta Histochemica et Cytochemica, Vol. 47, No. 6, 2014, p. 303-310.

Research output: Contribution to journalArticle

Nakamura, Tomomi ; Takagi, Sota ; Matsumoto, Midori ; Tashiro, Fumio ; Sakai, Tatsuo ; Ichimura, Koichiro. / Expression of Nephrin homologue in the freshwater planarian, Dugesia japonica. In: Acta Histochemica et Cytochemica. 2014 ; Vol. 47, No. 6. pp. 303-310.
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