Expression of neural RNA-binding proteins in the postnatal CNS

Implications of their roles in neuronal and glial cell development

Shin Ichi Sakakibara, Hideyuki Okano

Research output: Contribution to journalArticle

205 Citations (Scopus)

Abstract

There is an increasing interest in the role of RNA-binding proteins during neural development. Mouse-Musashi-1 (m-Msi-1) is a mouse neural RNA- binding protein with sequence similarity to Drosophila musashi (d-msi), which is essential for neural development. m-Msi-1 is highly enriched in neural precursor cells that are capable of generating both neurons and glia during embryonic CNS development. The present study characterized m-Msi-1-expressing cells in the postnatal and adult CNS. Postnatally, m-Msi-1 was expressed in proliferative neuronal precursors in the external granule cell layer of the cerebellum and in the anterior corner of the subventricular zone of the lateral ventricles. In gliogenesis, the persistent expression of m-Msi-1 was observed in cells of the astrocyte lineage ranging from proliferative glial precursors in the subventricular zone (SVZ), to differentiated astrocytes in the parenchyma. In addition, we showed that m-Mis-1 was still expressed in proliferating cells in the adult SVZ, which may contain neural precursor or stem cells. Another neural RNA-binding protein Hu (the mammalian homolog of Drosophila neuronal RNA-binding protein Elav) was present in postmitotic neurons throughout the development of the CNS, and its pattern of expression was compared with that of m-Msi-1. These observations imply that these two RNA-binding proteins may be involved in the development of neurons and glia by regulating gene expression at the post-transcriptional level.

Original languageEnglish
Pages (from-to)8300-8312
Number of pages13
JournalJournal of Neuroscience
Volume17
Issue number21
Publication statusPublished - 1997
Externally publishedYes

Fingerprint

RNA-Binding Proteins
Neuroglia
Lateral Ventricles
Neurons
Astrocytes
Drosophila
Cell Lineage
Cerebellum
Embryonic Development
Stem Cells
Gene Expression

Keywords

  • Astrocyte lineage
  • Drosophila musashi
  • Hu
  • Mouse-Musashi-1 (m-Msi- 1)
  • Neuronal and glial precursor cells
  • Postnatal CNS
  • RNA-binding proteins

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Expression of neural RNA-binding proteins in the postnatal CNS : Implications of their roles in neuronal and glial cell development. / Sakakibara, Shin Ichi; Okano, Hideyuki.

In: Journal of Neuroscience, Vol. 17, No. 21, 1997, p. 8300-8312.

Research output: Contribution to journalArticle

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