Expression of nuclear receptor mRNA and liver X receptor-mediated regulation of ABC transporter A1 at rat blood-brain barrier

Shin ichi Akanuma, Satoko Hori, Sumio Ohtsuki, Masachika Fujiyoshi, Tetsuya Terasaki

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The aim of the present study was to investigate the expression of nuclear receptor mRNA and regulation of the expression of ATP-binding cassette (ABC) transporters by nuclear receptor agonists in rat brain capillary endothelial cells, which form the blood-brain barrier, by using rat brain capillary fraction from 8-week-old rats and a conditionally immortalized brain capillary endothelial cell line (TR-BBB13). RT-PCR analysis revealed that liver X receptor α and β, retinoid X receptor α and β and peroxisome proliferator-activating receptor α and β mRNAs were expressed in the rat brain capillary endothelial cells and TR-BBB cells. In contrast, pregnane X receptor, farnesoid X receptor and constitutive androstane receptor were not detected. Furthermore, treatment with a liver X receptor agonist increased the ABCA1 mRNA level in TR-BBB13 cells, while ABCG2 mRNA expression was not affected. Treatment with a rat pregnane X receptor agonist did not affect the ABCB1 mRNA level in TR-BBB13 cells. These results demonstrate that the rat blood-brain barrier has an expressional regulation mechanism via sterol-related nuclear receptor, and indicate that the blood-brain barrier in 8-week-old rats lacks ABCB1 regulation via pregnane X receptor.

Original languageEnglish
Pages (from-to)669-674
Number of pages6
JournalNeurochemistry International
Volume52
Issue number4-5
DOIs
Publication statusPublished - 2008 Mar 1
Externally publishedYes

Fingerprint

ATP-Binding Cassette Transporters
Cytoplasmic and Nuclear Receptors
Blood-Brain Barrier
Messenger RNA
Endothelial Cells
Brain
Peroxisome Proliferators
Retinoid X Receptors
Sterols
Liver X Receptors
Cell Line
Polymerase Chain Reaction
pregnane X receptor

Keywords

  • ABC transporter
  • Blood-brain barrier
  • Brain capillary endothelial cell
  • LXR
  • Nuclear receptor
  • PPAR
  • PXR

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Expression of nuclear receptor mRNA and liver X receptor-mediated regulation of ABC transporter A1 at rat blood-brain barrier. / Akanuma, Shin ichi; Hori, Satoko; Ohtsuki, Sumio; Fujiyoshi, Masachika; Terasaki, Tetsuya.

In: Neurochemistry International, Vol. 52, No. 4-5, 01.03.2008, p. 669-674.

Research output: Contribution to journalArticle

Akanuma, Shin ichi ; Hori, Satoko ; Ohtsuki, Sumio ; Fujiyoshi, Masachika ; Terasaki, Tetsuya. / Expression of nuclear receptor mRNA and liver X receptor-mediated regulation of ABC transporter A1 at rat blood-brain barrier. In: Neurochemistry International. 2008 ; Vol. 52, No. 4-5. pp. 669-674.
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