Expression of ovary-specific acidic protein in steroidogenic tissues

A possible role in steroidogenesis

Tadashi Matsumoto, Kazuhiro Minegishi, Hitoshi Ishimoto, Mamoru Tanaka, Jon D. Hennebold, Takahide Teranishi, Yoshihisa Hattori, Masataka Furuya, Takayuki Higuchi, Satoshi Asai, Hye Kim Seon, Kei Miyakoshi, Yasunori Yoshimura

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Ovary-specific acidic protein (OSAP) is a novel molecule discovered from a genomic project designed to identify ovary-selective genes in mice. Whereas public databases suggest extraovarian expression of OSAP, its tissue distribution has not yet been well documented. Thus, the expression profile of mouse and human OSAP was determined by quantitative real-time RT-PCR using RNAs isolated from various tissues. The results demonstrate that the human and mouse OSAP expression profiles are similar; OSAP is prominently expressed in steroidogenic tissues with the highest level of expression observed in the adrenal gland. Placenta served as an exception and possessed minimal level of OSAP mRNA. Immunohistochemical studies show that mouse OSAP localizes almost exclusively to the steroid-producing cells of the ovary, adrenal gland, and testis. Consistent with predictions made by several subcellular localization algorithms, dual labeling studies in Y-1 mouse adrenocortical cells indicate OSAP resides in the mitochondria. Because of its abundant expression in steroidogenic cells and mitochondrial localization, a role for OSAP in steroidogenesis was determined. OSAP silencing by specific small interfering RNAs significantly inhibits 8-bromoadenosine-cAMP-induced progesterone production in Y-1 cells. Reduction in OSAP levels results in mitochondrial fragmentation and a decrease in the cellular content of mitochondrial DNA, indicative of decreased mitochondrial abundance. Lastly, 8-bromoadenosine-cAMP does not regulate OSAP protein expression in Y-1 cells as is the case for other steroidogenic components known to be induced by cAMP. Collectively these results suggest that OSAP is involved in steroidogenesis, potentially through its ability to maintain mitochondrial abundance and morphology.

Original languageEnglish
Pages (from-to)3353-3359
Number of pages7
JournalEndocrinology
Volume150
Issue number7
DOIs
Publication statusPublished - 2009 Jul

Fingerprint

Ovary
Proteins
Adrenal Glands
Tissue Distribution
Mitochondrial DNA
Placenta
Small Interfering RNA
Progesterone
Testis
Real-Time Polymerase Chain Reaction
Mitochondria
Steroids
Databases
RNA
Messenger RNA

ASJC Scopus subject areas

  • Endocrinology

Cite this

Matsumoto, T., Minegishi, K., Ishimoto, H., Tanaka, M., Hennebold, J. D., Teranishi, T., ... Yoshimura, Y. (2009). Expression of ovary-specific acidic protein in steroidogenic tissues: A possible role in steroidogenesis. Endocrinology, 150(7), 3353-3359. https://doi.org/10.1210/en.2008-1584

Expression of ovary-specific acidic protein in steroidogenic tissues : A possible role in steroidogenesis. / Matsumoto, Tadashi; Minegishi, Kazuhiro; Ishimoto, Hitoshi; Tanaka, Mamoru; Hennebold, Jon D.; Teranishi, Takahide; Hattori, Yoshihisa; Furuya, Masataka; Higuchi, Takayuki; Asai, Satoshi; Seon, Hye Kim; Miyakoshi, Kei; Yoshimura, Yasunori.

In: Endocrinology, Vol. 150, No. 7, 07.2009, p. 3353-3359.

Research output: Contribution to journalArticle

Matsumoto, T, Minegishi, K, Ishimoto, H, Tanaka, M, Hennebold, JD, Teranishi, T, Hattori, Y, Furuya, M, Higuchi, T, Asai, S, Seon, HK, Miyakoshi, K & Yoshimura, Y 2009, 'Expression of ovary-specific acidic protein in steroidogenic tissues: A possible role in steroidogenesis', Endocrinology, vol. 150, no. 7, pp. 3353-3359. https://doi.org/10.1210/en.2008-1584
Matsumoto, Tadashi ; Minegishi, Kazuhiro ; Ishimoto, Hitoshi ; Tanaka, Mamoru ; Hennebold, Jon D. ; Teranishi, Takahide ; Hattori, Yoshihisa ; Furuya, Masataka ; Higuchi, Takayuki ; Asai, Satoshi ; Seon, Hye Kim ; Miyakoshi, Kei ; Yoshimura, Yasunori. / Expression of ovary-specific acidic protein in steroidogenic tissues : A possible role in steroidogenesis. In: Endocrinology. 2009 ; Vol. 150, No. 7. pp. 3353-3359.
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