Expression of P-glycoprotein in adult T-cell leukemia cells

K. Yasuo, H. Shuichi, F. Tatsuhiko, Y. Akihiko, S. Tomoyuki, U. Atae, I. Kazuaki, S. Takeshi, U. Kimiharu, M. Masao, I. Minoru, A. Terukatsu, A. Shin-ichi

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

We have examined the expression of P-glycoprotein (P-gp) in adult T-cell leukemia (ATL) samples from 25 patients. Based on immunoblotting with a monoclonal antibody against P-gp, C219, 8 of 20 ATL patients were P-gp positive at the initial presentation. All 6 patients at the relapsed stage were P-gp positive, and refractory to chemotherapy. The expression of MDR1 mRNA in P-gp-positive ATL cells was increased at the relapsed stage of one patient. P-gp of this patient was photolabeled with [3H]azidopine and the labeling was inhibited with nimodipine, vinblastine and progesterone. These results suggest that P-gp expressed in ATL cells from patients at relapsed stage has the same binding site(s) for the drugs as that in multidrug resistant cells, and is correlated with the refractory nature of the cells to chemotherapy.

Original languageEnglish
Pages (from-to)2065-2071
Number of pages7
JournalBlood
Volume76
Issue number10
DOIs
Publication statusPublished - 1990
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Fingerprint Dive into the research topics of 'Expression of P-glycoprotein in adult T-cell leukemia cells'. Together they form a unique fingerprint.

  • Cite this

    Yasuo, K., Shuichi, H., Tatsuhiko, F., Akihiko, Y., Tomoyuki, S., Atae, U., Kazuaki, I., Takeshi, S., Kimiharu, U., Masao, M., Minoru, I., Terukatsu, A., & Shin-ichi, A. (1990). Expression of P-glycoprotein in adult T-cell leukemia cells. Blood, 76(10), 2065-2071. https://doi.org/10.1182/blood.v76.10.2065.2065