Expression of P-glycoprotein in adult T-cell leukemia cells

Yasuo Kuwazuru, Shuichi Hanada, Tatsuhiko Furukawa, Akihiko Yoshimura, Tomoyuki Sumizawa, Atae Utsunomiya, Kazuaki Ishibashi, Takeshi Saito, Kimiharu Uozumi, Masao Maruyama, Minoru Ishizawa, Terukatsu Arima, Shin Ichi Akiyama

Research output: Contribution to journalArticle

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Abstract

We have examined the expression of P-glycoprotein (P-gp) in adult T-cell leukemia (ATL) samples from 25 patients. Based on immunoblotting with a monoclonal antibody against P-gp, C219,8 of 20 ATL patients were P-gp positive at the initial presentation. All 6 patients at the relapsed stage were P-gp positive, and refractory to chemotherapy. The expression of MDR1 mRNA in P-gp-positive ATL cells was increased at the relapsed stage of one patient. P-gp of this patient was photolabeled with [3H]azidopine and the labeling was inhibited with nimodipine, vinblastine and progesterone. These results suggest that P-gp expressed in ATL cells from patients at relapsed stage has the same binding site(s) for the drugs as that in multidrug resistant cells, and is correlated with the refractory nature of the cells to chemotherapy.

Original languageEnglish
Pages (from-to)2065-2071
Number of pages7
JournalBlood
Volume76
Issue number10
Publication statusPublished - 1990 Nov 15
Externally publishedYes

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Adult T Cell Leukemia Lymphoma
T-cells
P-Glycoprotein
Chemotherapy
Refractory materials
Drug Therapy
Nimodipine
Vinblastine
Immunoblotting
Labeling
Progesterone
Binding Sites
Monoclonal Antibodies
Messenger RNA
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Hematology

Cite this

Kuwazuru, Y., Hanada, S., Furukawa, T., Yoshimura, A., Sumizawa, T., Utsunomiya, A., ... Akiyama, S. I. (1990). Expression of P-glycoprotein in adult T-cell leukemia cells. Blood, 76(10), 2065-2071.

Expression of P-glycoprotein in adult T-cell leukemia cells. / Kuwazuru, Yasuo; Hanada, Shuichi; Furukawa, Tatsuhiko; Yoshimura, Akihiko; Sumizawa, Tomoyuki; Utsunomiya, Atae; Ishibashi, Kazuaki; Saito, Takeshi; Uozumi, Kimiharu; Maruyama, Masao; Ishizawa, Minoru; Arima, Terukatsu; Akiyama, Shin Ichi.

In: Blood, Vol. 76, No. 10, 15.11.1990, p. 2065-2071.

Research output: Contribution to journalArticle

Kuwazuru, Y, Hanada, S, Furukawa, T, Yoshimura, A, Sumizawa, T, Utsunomiya, A, Ishibashi, K, Saito, T, Uozumi, K, Maruyama, M, Ishizawa, M, Arima, T & Akiyama, SI 1990, 'Expression of P-glycoprotein in adult T-cell leukemia cells', Blood, vol. 76, no. 10, pp. 2065-2071.
Kuwazuru Y, Hanada S, Furukawa T, Yoshimura A, Sumizawa T, Utsunomiya A et al. Expression of P-glycoprotein in adult T-cell leukemia cells. Blood. 1990 Nov 15;76(10):2065-2071.
Kuwazuru, Yasuo ; Hanada, Shuichi ; Furukawa, Tatsuhiko ; Yoshimura, Akihiko ; Sumizawa, Tomoyuki ; Utsunomiya, Atae ; Ishibashi, Kazuaki ; Saito, Takeshi ; Uozumi, Kimiharu ; Maruyama, Masao ; Ishizawa, Minoru ; Arima, Terukatsu ; Akiyama, Shin Ichi. / Expression of P-glycoprotein in adult T-cell leukemia cells. In: Blood. 1990 ; Vol. 76, No. 10. pp. 2065-2071.
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