Expression of prostaglandin E receptor EP 4 subtype in rat adrenal zona glomerulosa: Involvement in aldosterone release

Seiji Muro, Issei Tanaka, Takeshi Usui, Masato Kotani, Shigeki Koide, Masashi Mukoyama, Junichi Fukata, Hiroshi Itoh, Shuh Narumiya, Mitsuhiro Kawata, Kazuwa Nakao

Research output: Contribution to journalArticle

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Abstract

We examined the role of prostaglandin (PG) E receptors in the secretion of aldosterone. PGE 2 is known to exert its various biological functions by binding to PGE receptors. There are four subtypes of PGE receptors, EP 1, EP 2, EP 3, and EP 4. Among the PGE receptors EP 2 and EP 4 subtypes are coupled to Gs protein and stimulate adenylyl cyclase. In this study, PGE 2 caused adose-dependent increase in aldosterone production from the rat adrenal zona glomerulosa cells in vitro accompanied with an increase in intracellular cAMP concentration. A specific agonist for EP 2, butaprost, did not increase the cAMP production or the aldosterone release, suggesting the possibility that EP 4 mediates the secretion of aldosterone by PGE 2. Northern blot hybridization analysis disclosed that EP 4 gene was expressed in the rat adrenal gland but that EP 2 gene was not. In situ hybridization revealed that EP 4 mRNA is present abundantly in the zona glomerulosa of rat adrenal gland. These findings suggest that the PGE 2-EP 4 system is involved in the regulation of aldosterone secretion from the rat adrenal gland.

Original languageEnglish
Pages (from-to)429-436
Number of pages8
JournalEndocrine Journal
Volume47
Issue number4
Publication statusPublished - 2000
Externally publishedYes

Fingerprint

Receptors, Prostaglandin E, EP4 Subtype
Zona Glomerulosa
Prostaglandin E Receptors
Aldosterone
Prostaglandins E
Adrenal Glands
Adenylyl Cyclases
Northern Blotting
Genes
In Situ Hybridization
Messenger RNA

Keywords

  • Adrenal gland
  • Aldosterone
  • Prostaglandin

ASJC Scopus subject areas

  • Endocrinology

Cite this

Muro, S., Tanaka, I., Usui, T., Kotani, M., Koide, S., Mukoyama, M., ... Nakao, K. (2000). Expression of prostaglandin E receptor EP 4 subtype in rat adrenal zona glomerulosa: Involvement in aldosterone release. Endocrine Journal, 47(4), 429-436.

Expression of prostaglandin E receptor EP 4 subtype in rat adrenal zona glomerulosa : Involvement in aldosterone release. / Muro, Seiji; Tanaka, Issei; Usui, Takeshi; Kotani, Masato; Koide, Shigeki; Mukoyama, Masashi; Fukata, Junichi; Itoh, Hiroshi; Narumiya, Shuh; Kawata, Mitsuhiro; Nakao, Kazuwa.

In: Endocrine Journal, Vol. 47, No. 4, 2000, p. 429-436.

Research output: Contribution to journalArticle

Muro, S, Tanaka, I, Usui, T, Kotani, M, Koide, S, Mukoyama, M, Fukata, J, Itoh, H, Narumiya, S, Kawata, M & Nakao, K 2000, 'Expression of prostaglandin E receptor EP 4 subtype in rat adrenal zona glomerulosa: Involvement in aldosterone release', Endocrine Journal, vol. 47, no. 4, pp. 429-436.
Muro, Seiji ; Tanaka, Issei ; Usui, Takeshi ; Kotani, Masato ; Koide, Shigeki ; Mukoyama, Masashi ; Fukata, Junichi ; Itoh, Hiroshi ; Narumiya, Shuh ; Kawata, Mitsuhiro ; Nakao, Kazuwa. / Expression of prostaglandin E receptor EP 4 subtype in rat adrenal zona glomerulosa : Involvement in aldosterone release. In: Endocrine Journal. 2000 ; Vol. 47, No. 4. pp. 429-436.
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