Expression of prostaglandin E receptor EP4 subtype in rat adrenal zona glomerulosa: Involvement in aldosterone release

Seiji Muro, Issei Tanaka, Takeshi Usui, Masato Kotani, Shigeki Koide, Masashi Mukoyama, Junichi Fukata, Hiroshi Itoh, Shuh Narumiya, Mitsuhiro Kawata, Kazuwa Nakao

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)


We examined the role of prostaglandin (PG) E receptors in the secretion of aldosterone. PGE2 is known to exert its various biological functions by binding to PGE receptors. There are four subtypes of PGE receptors, EP1, EP2, EP3, and EP4. Among the PGE receptors EP2 and EP4 subtypes are coupled to Gs protein and stimulate adenylyl cyclase. In this study, PGE2 caused adose-dependent increase in aldosterone production from the rat adrenal zona glomerulosa cells in vitro accompanied with an increase in intracellular cAMP concentration. A specific agonist for EP2, butaprost, did not increase the cAMP production or the aldosterone release, suggesting the possibility that EP4 mediates the secretion of aldosterone by PGE2. Northern blot hybridization analysis disclosed that EP4 gene was expressed in the rat adrenal gland but that EP2 gene was not. In situ hybridization revealed that EP4 mRNA is present abundantly in the zona glomerulosa of rat adrenal gland. These findings suggest that the PGE2-EP4 system is involved in the regulation of aldosterone secretion from the rat adrenal gland.

Original languageEnglish
Pages (from-to)429-436
Number of pages8
JournalEndocrine journal
Issue number4
Publication statusPublished - 2000 Jan 1
Externally publishedYes


  • Adrenal gland
  • Aldosterone
  • Prostaglandin

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology


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