Expression of RNA-binding protein Musashi in hair follicle development and hair cycle progression

Yoriko Sugiyama-Nakagiri, Masashi Akiyama, Shinsuke Shibata, Hideyuki Okano, Hiroshi Shimizu

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Epithelial stem cells reside in the hair follicle (HF) bulge region and possess the ability to differentiate into a variety of cutaneous epithelial cells. The evolutionarily conserved Musashi family of RNA-binding proteins is associated with maintenance and/or asymmetric cell division of neural progenitor cells, and a mammalian Musashi protein is expressed in various epithelial stem/progenitor cells, including gut, stomach, and mammary gland. Thus, we hypothesized that Musashi might be expressed to stem cells and early progenitor cells of HF epithelium. Reverse transcriptase-polymerase chain reaction and immunoblotting identified Musashi-1 (Msl-1) and Musashi-2 (Msi-2) mRNA and protein in cultured mouse keratinocytes, but only Msi-1 was identified in human keratinocytes. In mice, immunohistochemical studies showed that Msi-1 and Msi-2 were expressed in the epidermis and HFs from E14.5 until adulthood. In the early anagen phase, Msi-1 and Msi-2 were expressed in the bulge and secondary germ cells and subsequently in inner root sheath (IRS) cells, especially the middle IRS cells, during the late anagen phase. In human skin, MsM was detected in fetal HF cells but not in adult HFs. These observations suggest that Musashi functions not only in the asymmetric division of early progenitor cells but also in the differentiation of IKS cells during HF development and hair cycle progression.

Original languageEnglish
Pages (from-to)80-92
Number of pages13
JournalAmerican Journal of Pathology
Volume168
Issue number1
DOIs
Publication statusPublished - 2006 Jan

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RNA-Binding Proteins
Hair Follicle
Hair
Stem Cells
Epithelial Cells
Keratinocytes
Asymmetric Cell Division
Skin
Human Mammary Glands
Reverse Transcriptase Polymerase Chain Reaction
Immunoblotting
Epidermis
Germ Cells
Cell Differentiation
Stomach
Proteins
Epithelium
Maintenance
Messenger RNA

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Expression of RNA-binding protein Musashi in hair follicle development and hair cycle progression. / Sugiyama-Nakagiri, Yoriko; Akiyama, Masashi; Shibata, Shinsuke; Okano, Hideyuki; Shimizu, Hiroshi.

In: American Journal of Pathology, Vol. 168, No. 1, 01.2006, p. 80-92.

Research output: Contribution to journalArticle

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