Expression of sialosyl-Tn in colony-forming unit-erythroid, erythroblasts, B cells, and a subset of CD4+ cells

Kazuo Muroi, Toshio Suda, Mitsuru Nakamura, Seiji Okada, Hisao Nojiri, Youichi Amemiya, Yasusada Miura, Sen Itiroh Hakomori

Research output: Contribution to journalArticle

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Abstract

The epitopes Tn and sialosyl-Tn are expressed on erythrocytes of individuals with a very rare blood group, who often suffer from 'Tn syndrome.' We surveyed expression of Tn and sialosyl-Tn in normal blood cells, malignant transformed cells, and progenitor stem cells from bone marrow (BM). An anti-Tn antibody, IE3, and an anti-sialosyl-Tn antibody, TKH2, were used in this study. TKH2 reacted with erythroblasts, B cells, and a subset of CD4+ cells, but not with erythrocytes. Erythroblastic cell lines (K562, HEL, and UT7/EPO) and B-cell lines (Daudi, Raji, and B-cell lines transformed by Epstein-Barr virus) showed reactivity to TKH2. Similar results from the reactivity of TKH2 with transformed cells from leukemia patients and lymphoma patients were obtained; TKH2 reacted with blasts from erythroleukemia (M6; for 4 of 4 cases) and with lymphocytes from B-cell chronic lymphocytic leukemia (3 of 3), B-cell lymphoma (5 of 5), and CD4+ adult T-cell leukemia (4 of 4), but did not react with blasts from acute myeloid leukemia (M0 to M5; 0 of 22) or acute lymphoid leukemia (B-lymphoid leukemia, 0 of 11; T-lymphoid leukemia, 0 of 2; undifferentiated leukemia, 0 of 1). IE3 did not react with all of the tested cells. CD2- CD19-TKH2+ normal BM cells (BMC) contained blasts and various maturation stages of erythroblasts. The TKH2+ cells produced a large number of colony-forming unit-erythroid (CFU-E) colonies, whereas they produced a small number of burst-forming unit-erythroid colonies and CFU-granulocyte-macrophage colonies. CD34+ normal BMC did not express Tn and sialosyl-Tn. These findings suggest that sialosyl-Tn expresses in CFU-E to erythroblasts.

Original languageEnglish
Pages (from-to)84-91
Number of pages8
JournalBlood
Volume83
Issue number1
Publication statusPublished - 1994 Jan 1

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B-Lymphocyte Subsets
Erythroblasts
Erythroid Precursor Cells
Cells
Lymphoid Leukemia
Cell Line
Leukemia
B-Lymphocytes
Stem Cells
Erythrocytes
Bone
Adult T Cell Leukemia Lymphoma
Leukemia, Erythroblastic, Acute
B-Cell Lymphoma
B-Cell Chronic Lymphocytic Leukemia
Blood Group Antigens
T-cells
Lymphocytes
Human Herpesvirus 4
Antibodies

ASJC Scopus subject areas

  • Hematology

Cite this

Muroi, K., Suda, T., Nakamura, M., Okada, S., Nojiri, H., Amemiya, Y., ... Hakomori, S. I. (1994). Expression of sialosyl-Tn in colony-forming unit-erythroid, erythroblasts, B cells, and a subset of CD4+ cells. Blood, 83(1), 84-91.

Expression of sialosyl-Tn in colony-forming unit-erythroid, erythroblasts, B cells, and a subset of CD4+ cells. / Muroi, Kazuo; Suda, Toshio; Nakamura, Mitsuru; Okada, Seiji; Nojiri, Hisao; Amemiya, Youichi; Miura, Yasusada; Hakomori, Sen Itiroh.

In: Blood, Vol. 83, No. 1, 01.01.1994, p. 84-91.

Research output: Contribution to journalArticle

Muroi, K, Suda, T, Nakamura, M, Okada, S, Nojiri, H, Amemiya, Y, Miura, Y & Hakomori, SI 1994, 'Expression of sialosyl-Tn in colony-forming unit-erythroid, erythroblasts, B cells, and a subset of CD4+ cells', Blood, vol. 83, no. 1, pp. 84-91.
Muroi K, Suda T, Nakamura M, Okada S, Nojiri H, Amemiya Y et al. Expression of sialosyl-Tn in colony-forming unit-erythroid, erythroblasts, B cells, and a subset of CD4+ cells. Blood. 1994 Jan 1;83(1):84-91.
Muroi, Kazuo ; Suda, Toshio ; Nakamura, Mitsuru ; Okada, Seiji ; Nojiri, Hisao ; Amemiya, Youichi ; Miura, Yasusada ; Hakomori, Sen Itiroh. / Expression of sialosyl-Tn in colony-forming unit-erythroid, erythroblasts, B cells, and a subset of CD4+ cells. In: Blood. 1994 ; Vol. 83, No. 1. pp. 84-91.
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abstract = "The epitopes Tn and sialosyl-Tn are expressed on erythrocytes of individuals with a very rare blood group, who often suffer from 'Tn syndrome.' We surveyed expression of Tn and sialosyl-Tn in normal blood cells, malignant transformed cells, and progenitor stem cells from bone marrow (BM). An anti-Tn antibody, IE3, and an anti-sialosyl-Tn antibody, TKH2, were used in this study. TKH2 reacted with erythroblasts, B cells, and a subset of CD4+ cells, but not with erythrocytes. Erythroblastic cell lines (K562, HEL, and UT7/EPO) and B-cell lines (Daudi, Raji, and B-cell lines transformed by Epstein-Barr virus) showed reactivity to TKH2. Similar results from the reactivity of TKH2 with transformed cells from leukemia patients and lymphoma patients were obtained; TKH2 reacted with blasts from erythroleukemia (M6; for 4 of 4 cases) and with lymphocytes from B-cell chronic lymphocytic leukemia (3 of 3), B-cell lymphoma (5 of 5), and CD4+ adult T-cell leukemia (4 of 4), but did not react with blasts from acute myeloid leukemia (M0 to M5; 0 of 22) or acute lymphoid leukemia (B-lymphoid leukemia, 0 of 11; T-lymphoid leukemia, 0 of 2; undifferentiated leukemia, 0 of 1). IE3 did not react with all of the tested cells. CD2- CD19-TKH2+ normal BM cells (BMC) contained blasts and various maturation stages of erythroblasts. The TKH2+ cells produced a large number of colony-forming unit-erythroid (CFU-E) colonies, whereas they produced a small number of burst-forming unit-erythroid colonies and CFU-granulocyte-macrophage colonies. CD34+ normal BMC did not express Tn and sialosyl-Tn. These findings suggest that sialosyl-Tn expresses in CFU-E to erythroblasts.",
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AU - Suda, Toshio

AU - Nakamura, Mitsuru

AU - Okada, Seiji

AU - Nojiri, Hisao

AU - Amemiya, Youichi

AU - Miura, Yasusada

AU - Hakomori, Sen Itiroh

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