Expression of sialylated Lewis(x) gangliosides in cultured lens epithelial cells from rhesus monkey

Manabu Ogiso, Makoto Takehana, Shizuko Kobayashi, Motonori Hoshi

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Monkey and human lenses contain essentially the same glycosphingolipids, and Lewis(x) and sialylated Lewis(x) epitopes are expressed on the terminal structure of neolactotetraosylceramide. However, monolayer cultures of lens epithelial cells from rhesus monkey expressed gangliosides GM3, GD3 and a small amount of GM1, but not sialylated Lewis(x) epitopes. Eight-week-old cultures on various extracellular matrices resulted in morphological changes in lens epithelial cells. Monolayer of cells cultured on vitronectin or polylysine assembled into aggregates after 4 weeks of culture. Cells cultured on vitronectin expressed sialyl-Lewis(x) gangliosides and did not exhibit GD3. On collagens, fibronectin and laminin elongated cells were observed in cells cultured for 8 weeks. Thus, the interaction between cells and extracellular matrices influenced morphology and glycosphingolipid composition in lens epithelial cells.

Original languageEnglish
Pages (from-to)765-773
Number of pages9
JournalExperimental Eye Research
Volume66
Issue number6
DOIs
Publication statusPublished - 1998 Jun

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Keywords

  • Ganglioside
  • Glycosphingolipid
  • Lens epithelial cell
  • Rhesus monkey
  • Sialyl-Lewis(x)

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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