Expression of Siamois and Twin in the blastula Chordin/Noggin signaling center is required for brain formation in Xenopus laevis embryos

Hideyuki Ishibashi, Noriko Matsumura, Hiroshi Hanafusa, Kunihiro Matsumoto, E. M De Robertis, Hiroki Kuroda

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The blastula Chordin- and Noggin-expressing (BCNE) center located in the dorsal animal region of the Xenopus blastula embryo contains both prospective anterior neuroectoderm and Spemann organizer precursor cells. Here we show that, contrary to previous reports, the canonical Wnt target homeobox genes, Double knockdown of these genes using antisense morpholinos in Xenopus laevis blocked head formation, reduced the expression of the other BCNE center genes, upregulated Bmp4 expression, and nullified hyperdorsalization by lithium chloride. Moreover, gain- and loss-of-function experiments showed that Siamois and Twin expression is repressed by the vegetal transcription factor VegT. We propose that VegT expression causes maternal β-Catenin signals to restrict Siamois and Twin expression to the BCNE region. A two-step inhibition of BMP signals by Siamois and Twin - first by transcriptional repression of Bmp4 and then by activation of the expression of the BMP inhibitors Chordin and Noggin - in the BCNE center is required for head formation.

Original languageEnglish
Pages (from-to)58-66
Number of pages9
JournalMechanisms of Development
Volume125
Issue number1-2
DOIs
Publication statusPublished - 2008 Jan
Externally publishedYes

Fingerprint

Blastula
Xenopus laevis
Embryonic Structures
Brain
Embryonic Organizers
Head
Gene Knockdown Techniques
Neural Plate
Lithium Chloride
Catenins
Morpholinos
Homeobox Genes
Xenopus
Genes
Transcription Factors
Mothers
chordin

Keywords

  • BMP
  • Cell signaling
  • Chordin
  • Noggin
  • Siamois
  • Spemann organizer
  • Twin
  • Wnt
  • Xenopus

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

Cite this

Expression of Siamois and Twin in the blastula Chordin/Noggin signaling center is required for brain formation in Xenopus laevis embryos. / Ishibashi, Hideyuki; Matsumura, Noriko; Hanafusa, Hiroshi; Matsumoto, Kunihiro; Robertis, E. M De; Kuroda, Hiroki.

In: Mechanisms of Development, Vol. 125, No. 1-2, 01.2008, p. 58-66.

Research output: Contribution to journalArticle

Ishibashi, Hideyuki ; Matsumura, Noriko ; Hanafusa, Hiroshi ; Matsumoto, Kunihiro ; Robertis, E. M De ; Kuroda, Hiroki. / Expression of Siamois and Twin in the blastula Chordin/Noggin signaling center is required for brain formation in Xenopus laevis embryos. In: Mechanisms of Development. 2008 ; Vol. 125, No. 1-2. pp. 58-66.
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