Expression of stem cell marker and receptor kinase genes in glioblastoma tissue quantified by real-time RT-PCR

Koji Yoshimoto, Xinlong Ma, Yaulei Guan, Masahiro Mizoguchi, Akira Nakamizo, Toshiyuki Amano, Nobuhiro Hata, Daisuke Kuga, Tomio Sasaki

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Glioblastoma is dependent on a specific signaling pathway to maintain its tumor phenotype. The receptor tyrosine kinase (RTK) family mediates the multiple oncogenic growth factor receptor signaling and contributes to the pathogenesis of glioblastoma. Recently, many studies have shown that the expression of stem cell marker in glioblastoma tissue has prognostic significance, which indicates that the quantification of stem cell markers and RTK genes yields biological information about glioblastoma. In this study, we quantified RNA expression levels of stem cell markers [CD133, Nestin, BMI-1, maternal embryonic leucine zipper kinase (MELK), and Notch1-4] as well as RTKs (EGFR, ErbB4, VEGFR1-3, FGFR1, -2, PDGFRA, and PDGFRB) in 42 clinical samples of glioblastoma by the real-time RT-PCR method. We demonstrated that the expression of MELK is exclusively upregulated in glioblastoma tissue. Notch receptor expression is moderately upregulated and is correlated with that of VEGFR2, VEGFR3, and PDGFR β. Unsupervised clustering identified one unique sample group that showed high expression of most of the genes analyzed. Our results suggest that quantification of these stem cell markers and RTK genes can stratify patients based on the expression profile, which might provide insight into the glioma biology in each cluster.

Original languageEnglish
Pages (from-to)291-296
Number of pages6
JournalBrain Tumor Pathology
Volume28
Issue number4
DOIs
Publication statusPublished - 2011 Oct 1
Externally publishedYes

Fingerprint

Glioblastoma
Real-Time Polymerase Chain Reaction
Phosphotransferases
Stem Cells
Receptor Protein-Tyrosine Kinases
Genes
Leucine Zippers
Mothers
Notch Receptors
Platelet-Derived Growth Factor beta Receptor
Nestin
Growth Factor Receptors
Glioma
Cluster Analysis
RNA
Phenotype
Gene Expression
Neoplasms

Keywords

  • Glioma
  • Quantification
  • Stem cell marker

ASJC Scopus subject areas

  • Oncology
  • Clinical Neurology
  • Cancer Research

Cite this

Yoshimoto, K., Ma, X., Guan, Y., Mizoguchi, M., Nakamizo, A., Amano, T., ... Sasaki, T. (2011). Expression of stem cell marker and receptor kinase genes in glioblastoma tissue quantified by real-time RT-PCR. Brain Tumor Pathology, 28(4), 291-296. https://doi.org/10.1007/s10014-011-0046-0

Expression of stem cell marker and receptor kinase genes in glioblastoma tissue quantified by real-time RT-PCR. / Yoshimoto, Koji; Ma, Xinlong; Guan, Yaulei; Mizoguchi, Masahiro; Nakamizo, Akira; Amano, Toshiyuki; Hata, Nobuhiro; Kuga, Daisuke; Sasaki, Tomio.

In: Brain Tumor Pathology, Vol. 28, No. 4, 01.10.2011, p. 291-296.

Research output: Contribution to journalArticle

Yoshimoto, K, Ma, X, Guan, Y, Mizoguchi, M, Nakamizo, A, Amano, T, Hata, N, Kuga, D & Sasaki, T 2011, 'Expression of stem cell marker and receptor kinase genes in glioblastoma tissue quantified by real-time RT-PCR', Brain Tumor Pathology, vol. 28, no. 4, pp. 291-296. https://doi.org/10.1007/s10014-011-0046-0
Yoshimoto, Koji ; Ma, Xinlong ; Guan, Yaulei ; Mizoguchi, Masahiro ; Nakamizo, Akira ; Amano, Toshiyuki ; Hata, Nobuhiro ; Kuga, Daisuke ; Sasaki, Tomio. / Expression of stem cell marker and receptor kinase genes in glioblastoma tissue quantified by real-time RT-PCR. In: Brain Tumor Pathology. 2011 ; Vol. 28, No. 4. pp. 291-296.
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