Expression of telomerase activity in thymoma and thymiccarcinoma tissues; a clinicopathological study

Masazumi Watanabe, M. Kawamura, H. Horinouchi, M. Mukai, K. Kobayashi

Research output: Contribution to journalArticle

Abstract

BACKGROUND: Telomerase is a nucleoprotein complex that caps the physical termini of all eukaryotic chromosomes. It is suggested that telomerase play important roles in unlimited cell division acquisition of the malignant phenotype. We studied the relation of telomerase activity in thymoma and thymic carcinoma to the clinicopathological features of these lesions. METHODS: Tissue specimens were surgically resected from patients with thymoma and thymic carcinoma. Telomerase activity was evaluated according to a modified telomeric repeat amplification protocol (TRAP) assay. RESULTS: Telomerase activity was detected in all thymic epithelial tumors. The activity (mean +/- SD: unit/microgram.protein) in thymoma (n = 17) was significantly higher than that in thymic carcinoma (n = 7) [431.8 +/- 400.1 vs. 68.8 +/- 39.8: p < 0.01]. Telomerase activities in thymoma and thymic carcinoma were significantly higher than that in primary lung adenocarcinoma (33.5 +/- 39.2: n = 47), studied as control (p < 0.01). In patients with thymoma, telomerase activity did not correlate with tumor stage according to Masaoka classification (p = 0.776). In patients with thymic carcinoma, however, telomerase activity positively correlated with tumor stage (p = 0.02). In thymoma, telomerase activity positively correlated with the ratio of induced lymphocytes according to Rosai's classification (p = 0.045). CONCLUSION: In thymoma, telomerase activity reflects the presence of immature T-cell lymphocytes in tumor tissue rather than tumor stage or malignant phenotype. In thymic carcinoma, telomerase activity derived directly from cancer cells may relate to tumor stage.

Original languageEnglish
Pages (from-to)945-948
Number of pages4
JournalKyobu geka. The Japanese journal of thoracic surgery
Volume55
Issue number11
Publication statusPublished - 2002

Fingerprint

Thymoma
Telomerase
Neoplasms
Lymphocytes
Phenotype
Nucleoproteins
Cell Division

Cite this

Expression of telomerase activity in thymoma and thymiccarcinoma tissues; a clinicopathological study. / Watanabe, Masazumi; Kawamura, M.; Horinouchi, H.; Mukai, M.; Kobayashi, K.

In: Kyobu geka. The Japanese journal of thoracic surgery, Vol. 55, No. 11, 2002, p. 945-948.

Research output: Contribution to journalArticle

Watanabe, M, Kawamura, M, Horinouchi, H, Mukai, M & Kobayashi, K 2002, 'Expression of telomerase activity in thymoma and thymiccarcinoma tissues; a clinicopathological study', Kyobu geka. The Japanese journal of thoracic surgery, vol. 55, no. 11, pp. 945-948.
Watanabe, Masazumi ; Kawamura, M. ; Horinouchi, H. ; Mukai, M. ; Kobayashi, K. / Expression of telomerase activity in thymoma and thymiccarcinoma tissues; a clinicopathological study. In: Kyobu geka. The Japanese journal of thoracic surgery. 2002 ; Vol. 55, No. 11. pp. 945-948.
@article{411b43334da844eaa30738a412434177,
title = "Expression of telomerase activity in thymoma and thymiccarcinoma tissues; a clinicopathological study",
abstract = "BACKGROUND: Telomerase is a nucleoprotein complex that caps the physical termini of all eukaryotic chromosomes. It is suggested that telomerase play important roles in unlimited cell division acquisition of the malignant phenotype. We studied the relation of telomerase activity in thymoma and thymic carcinoma to the clinicopathological features of these lesions. METHODS: Tissue specimens were surgically resected from patients with thymoma and thymic carcinoma. Telomerase activity was evaluated according to a modified telomeric repeat amplification protocol (TRAP) assay. RESULTS: Telomerase activity was detected in all thymic epithelial tumors. The activity (mean +/- SD: unit/microgram.protein) in thymoma (n = 17) was significantly higher than that in thymic carcinoma (n = 7) [431.8 +/- 400.1 vs. 68.8 +/- 39.8: p < 0.01]. Telomerase activities in thymoma and thymic carcinoma were significantly higher than that in primary lung adenocarcinoma (33.5 +/- 39.2: n = 47), studied as control (p < 0.01). In patients with thymoma, telomerase activity did not correlate with tumor stage according to Masaoka classification (p = 0.776). In patients with thymic carcinoma, however, telomerase activity positively correlated with tumor stage (p = 0.02). In thymoma, telomerase activity positively correlated with the ratio of induced lymphocytes according to Rosai's classification (p = 0.045). CONCLUSION: In thymoma, telomerase activity reflects the presence of immature T-cell lymphocytes in tumor tissue rather than tumor stage or malignant phenotype. In thymic carcinoma, telomerase activity derived directly from cancer cells may relate to tumor stage.",
author = "Masazumi Watanabe and M. Kawamura and H. Horinouchi and M. Mukai and K. Kobayashi",
year = "2002",
language = "English",
volume = "55",
pages = "945--948",
journal = "Japanese Journal of Thoracic Surgery",
issn = "0021-5252",
publisher = "Nankodo Co., Ltd.",
number = "11",

}

TY - JOUR

T1 - Expression of telomerase activity in thymoma and thymiccarcinoma tissues; a clinicopathological study

AU - Watanabe, Masazumi

AU - Kawamura, M.

AU - Horinouchi, H.

AU - Mukai, M.

AU - Kobayashi, K.

PY - 2002

Y1 - 2002

N2 - BACKGROUND: Telomerase is a nucleoprotein complex that caps the physical termini of all eukaryotic chromosomes. It is suggested that telomerase play important roles in unlimited cell division acquisition of the malignant phenotype. We studied the relation of telomerase activity in thymoma and thymic carcinoma to the clinicopathological features of these lesions. METHODS: Tissue specimens were surgically resected from patients with thymoma and thymic carcinoma. Telomerase activity was evaluated according to a modified telomeric repeat amplification protocol (TRAP) assay. RESULTS: Telomerase activity was detected in all thymic epithelial tumors. The activity (mean +/- SD: unit/microgram.protein) in thymoma (n = 17) was significantly higher than that in thymic carcinoma (n = 7) [431.8 +/- 400.1 vs. 68.8 +/- 39.8: p < 0.01]. Telomerase activities in thymoma and thymic carcinoma were significantly higher than that in primary lung adenocarcinoma (33.5 +/- 39.2: n = 47), studied as control (p < 0.01). In patients with thymoma, telomerase activity did not correlate with tumor stage according to Masaoka classification (p = 0.776). In patients with thymic carcinoma, however, telomerase activity positively correlated with tumor stage (p = 0.02). In thymoma, telomerase activity positively correlated with the ratio of induced lymphocytes according to Rosai's classification (p = 0.045). CONCLUSION: In thymoma, telomerase activity reflects the presence of immature T-cell lymphocytes in tumor tissue rather than tumor stage or malignant phenotype. In thymic carcinoma, telomerase activity derived directly from cancer cells may relate to tumor stage.

AB - BACKGROUND: Telomerase is a nucleoprotein complex that caps the physical termini of all eukaryotic chromosomes. It is suggested that telomerase play important roles in unlimited cell division acquisition of the malignant phenotype. We studied the relation of telomerase activity in thymoma and thymic carcinoma to the clinicopathological features of these lesions. METHODS: Tissue specimens were surgically resected from patients with thymoma and thymic carcinoma. Telomerase activity was evaluated according to a modified telomeric repeat amplification protocol (TRAP) assay. RESULTS: Telomerase activity was detected in all thymic epithelial tumors. The activity (mean +/- SD: unit/microgram.protein) in thymoma (n = 17) was significantly higher than that in thymic carcinoma (n = 7) [431.8 +/- 400.1 vs. 68.8 +/- 39.8: p < 0.01]. Telomerase activities in thymoma and thymic carcinoma were significantly higher than that in primary lung adenocarcinoma (33.5 +/- 39.2: n = 47), studied as control (p < 0.01). In patients with thymoma, telomerase activity did not correlate with tumor stage according to Masaoka classification (p = 0.776). In patients with thymic carcinoma, however, telomerase activity positively correlated with tumor stage (p = 0.02). In thymoma, telomerase activity positively correlated with the ratio of induced lymphocytes according to Rosai's classification (p = 0.045). CONCLUSION: In thymoma, telomerase activity reflects the presence of immature T-cell lymphocytes in tumor tissue rather than tumor stage or malignant phenotype. In thymic carcinoma, telomerase activity derived directly from cancer cells may relate to tumor stage.

UR - http://www.scopus.com/inward/record.url?scp=0036781210&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036781210&partnerID=8YFLogxK

M3 - Article

VL - 55

SP - 945

EP - 948

JO - Japanese Journal of Thoracic Surgery

JF - Japanese Journal of Thoracic Surgery

SN - 0021-5252

IS - 11

ER -