Expression of the adrenomedullin gene in adipose tissue

Takuo Nambu, Hiroshi Arai, Yasato Komatsu, Akihiro Yasoda, Kenji Moriyama, Naotetsu Kanamoto, Hiroshi Itoh, Kazuwa Nakao

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Adrenomedullin (AM) is a potent vasodilating peptide originally isolated from human pheochromocytoma cells. This report concerns the expression and secretion of AM from adipose tissue. Northern blot analysis demonstrated marked expression of AM mRNA in mouse adipose tissue. Expression levels in adipose tissues were 2.5-3.2 times higher than in the kidney. AM mRNA level in mature adipocytes was 7.3 times higher than in the stroma-vascular fraction of adipose tissue. In mature adipocyte culture, time-dependent increase of AM peptide concentration in the culture medium was detected. AM expression was also detected in human subcutaneous adipose tissue. Adipose AM expression significantly increased in obesity mouse model, high-fat diet fed mice and ob/ob mice. These results suggest that adipose tissue, especially mature adipocytes, is major source of AM in the body, and that adipocyte-derived AM plays a pathophysiological role in obesity.

Original languageEnglish
Pages (from-to)17-22
Number of pages6
JournalRegulatory Peptides
Volume132
Issue number1-3
DOIs
Publication statusPublished - 2005 Dec 15
Externally publishedYes

Keywords

  • Adiopocyte
  • Adrenomedullin
  • Fat
  • Obesity
  • ob/ob mice

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Clinical Biochemistry
  • Cellular and Molecular Neuroscience

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  • Cite this

    Nambu, T., Arai, H., Komatsu, Y., Yasoda, A., Moriyama, K., Kanamoto, N., Itoh, H., & Nakao, K. (2005). Expression of the adrenomedullin gene in adipose tissue. Regulatory Peptides, 132(1-3), 17-22. https://doi.org/10.1016/j.regpep.2005.07.006