Expression of the multidrug transporter, P-glycoprotein, in acute leukemia cells and correlation to clinical drug resistance

Y. Kuwazuru, Akihiko Yoshimura, S. Hanada, A. Utsunomiya, T. Makino, K. Ishibashi, M. Kodama, M. Iwahashi, T. Arima, S. I. Akiyama

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Abstract

The overexpression of a cell-surface glycoprotein termed P-glycoprotein (P-gp) is frequently associated with multi-drug resistance (MDR) in cell lines in vitro. To evaluate the implications of P-gp expression in clinical drug resistance, the authors examined the expression of P-gp in leukemia cells from patients with acute myelogenous leukemia (AML) and those with acute lymphoblastic leukemia (ALL) at initial presentation and relapse, using immunoblotting with a monoclonal antibody against P-gp, C219. Nine of 17 patients with AML and four of 11 patients with ALL had P-gp-positive results at the initial presentation, and most P-gp-positive patients did not respond to chemotherapy. Four of seven patients at the relapsed stage and all three patients with preceding myelodysplastic syndrome had P-gp-positive results. The expression of P-gp and clinical refractoriness to chemotherapy were highly correlated. These data indicate that the expression of P-gp is closely related to clinical drug resistance in acute leukemia.

Original languageEnglish
Pages (from-to)868-873
Number of pages6
JournalCancer
Volume66
Issue number5
Publication statusPublished - 1990
Externally publishedYes

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P-Glycoprotein
Drug Resistance
Leukemia
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Acute Myeloid Leukemia
Drug Therapy
Myelodysplastic Syndromes
Membrane Glycoproteins
Multiple Drug Resistance
Immunoblotting
Monoclonal Antibodies
Recurrence
Cell Line

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Kuwazuru, Y., Yoshimura, A., Hanada, S., Utsunomiya, A., Makino, T., Ishibashi, K., ... Akiyama, S. I. (1990). Expression of the multidrug transporter, P-glycoprotein, in acute leukemia cells and correlation to clinical drug resistance. Cancer, 66(5), 868-873.

Expression of the multidrug transporter, P-glycoprotein, in acute leukemia cells and correlation to clinical drug resistance. / Kuwazuru, Y.; Yoshimura, Akihiko; Hanada, S.; Utsunomiya, A.; Makino, T.; Ishibashi, K.; Kodama, M.; Iwahashi, M.; Arima, T.; Akiyama, S. I.

In: Cancer, Vol. 66, No. 5, 1990, p. 868-873.

Research output: Contribution to journalArticle

Kuwazuru, Y, Yoshimura, A, Hanada, S, Utsunomiya, A, Makino, T, Ishibashi, K, Kodama, M, Iwahashi, M, Arima, T & Akiyama, SI 1990, 'Expression of the multidrug transporter, P-glycoprotein, in acute leukemia cells and correlation to clinical drug resistance', Cancer, vol. 66, no. 5, pp. 868-873.
Kuwazuru, Y. ; Yoshimura, Akihiko ; Hanada, S. ; Utsunomiya, A. ; Makino, T. ; Ishibashi, K. ; Kodama, M. ; Iwahashi, M. ; Arima, T. ; Akiyama, S. I. / Expression of the multidrug transporter, P-glycoprotein, in acute leukemia cells and correlation to clinical drug resistance. In: Cancer. 1990 ; Vol. 66, No. 5. pp. 868-873.
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