Expression of the multidrug transporter, P-glycoprotein, in chronic myelogenous leukaemia cells in blast crisis

Y. Kuwazuru, Akihiko Yoshimura, S. Hanada, M. Ichikawa, T. Saito, K. Uozumi, A. Utsunomiya, T. Arima, S. I. Akiyama

Research output: Contribution to journalArticle

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Abstract

The overexpression of a cell-surface glycoprotein termed P-glycoprotein (P-gp) is frequently associated with multidrug resistance (MDR) in cell lines in vitro. To evaluate the implications of P-gp expression in clinical drug-resistance, we examined the expression of P-gp in fresh leukaemia cells from chronic myelogenous leukaemia (CML) patients in blast crisis. By using immunoblotting with a monoclonal antibody against P-gp, C219, we showed that leukaemia cells from three CML patients in blast crisis were P-gp negative at the stage when these patients were in complete remission and that the cells showed high levels of P-gp expression at times when the same patients had relapsed and had not responded to chemotherapy. Six out of 11 patients (nine in the refractory state) were P-gp positive and they rarely responded to chemotherapy. These data suggest that the expression of P-gp is closely associated with drug-resistance in CML.

Original languageEnglish
Pages (from-to)24-29
Number of pages6
JournalBritish Journal of Haematology
Volume74
Issue number1
Publication statusPublished - 1990
Externally publishedYes

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Blast Crisis
P-Glycoprotein
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Drug Resistance
Leukemia
Drug Therapy
Membrane Glycoproteins
Multiple Drug Resistance
Immunoblotting
Monoclonal Antibodies
Cell Line

ASJC Scopus subject areas

  • Hematology

Cite this

Kuwazuru, Y., Yoshimura, A., Hanada, S., Ichikawa, M., Saito, T., Uozumi, K., ... Akiyama, S. I. (1990). Expression of the multidrug transporter, P-glycoprotein, in chronic myelogenous leukaemia cells in blast crisis. British Journal of Haematology, 74(1), 24-29.

Expression of the multidrug transporter, P-glycoprotein, in chronic myelogenous leukaemia cells in blast crisis. / Kuwazuru, Y.; Yoshimura, Akihiko; Hanada, S.; Ichikawa, M.; Saito, T.; Uozumi, K.; Utsunomiya, A.; Arima, T.; Akiyama, S. I.

In: British Journal of Haematology, Vol. 74, No. 1, 1990, p. 24-29.

Research output: Contribution to journalArticle

Kuwazuru, Y, Yoshimura, A, Hanada, S, Ichikawa, M, Saito, T, Uozumi, K, Utsunomiya, A, Arima, T & Akiyama, SI 1990, 'Expression of the multidrug transporter, P-glycoprotein, in chronic myelogenous leukaemia cells in blast crisis', British Journal of Haematology, vol. 74, no. 1, pp. 24-29.
Kuwazuru, Y. ; Yoshimura, Akihiko ; Hanada, S. ; Ichikawa, M. ; Saito, T. ; Uozumi, K. ; Utsunomiya, A. ; Arima, T. ; Akiyama, S. I. / Expression of the multidrug transporter, P-glycoprotein, in chronic myelogenous leukaemia cells in blast crisis. In: British Journal of Haematology. 1990 ; Vol. 74, No. 1. pp. 24-29.
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