Expression of the multidrug transporter, P-glycoprotein, in chronic myelogenous leukaemia cells in blast crisis

Y. Kuwazuru, Akihiko Yoshimura, S. Hanada, M. Ichikawa, T. Saito, K. Uozumi, A. Utsunomiya, T. Arima, S. I. Akiyama

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Abstract

The overexpression of a cell-surface glycoprotein termed P-glycoprotein (P-gp) is frequently associated with multidrug resistance (MDR) in cell lines in vitro. To evaluate the implications of P-gp expression in clinical drug-resistance, we examined the expression of P-gp in fresh leukaemia cells from chronic myelogenous leukaemia (CML) patients in blast crisis. By using immunoblotting with a monoclonal antibody against P-gp, C219, we showed that leukaemia cells from three CML patients in blast crisis were P-gp negative at the stage when these patients were in complete remission and that the cells showed high levels of P-gp expression at times when the same patients had relapsed and had not responded to chemotherapy. Six out of 11 patients (nine in the refractory state) were P-gp positive and they rarely responded to chemotherapy. These data suggest that the expression of P-gp is closely associated with drug-resistance in CML.

Original languageEnglish
Pages (from-to)24-29
Number of pages6
JournalBritish Journal of Haematology
Volume74
Issue number1
Publication statusPublished - 1990
Externally publishedYes

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ASJC Scopus subject areas

  • Hematology

Cite this

Kuwazuru, Y., Yoshimura, A., Hanada, S., Ichikawa, M., Saito, T., Uozumi, K., Utsunomiya, A., Arima, T., & Akiyama, S. I. (1990). Expression of the multidrug transporter, P-glycoprotein, in chronic myelogenous leukaemia cells in blast crisis. British Journal of Haematology, 74(1), 24-29.