Expression of the multidrug transporter, P‐glycoprotein, in chronic myelogenous leukaemia cells in blast crisis

Yasuo Kuwazuru, Akihiko Yoshimura, Shuichi Hanada, Misako Ichikawa, Takeshi Saito, Kimiharu Uozumi, Atae Utsunomiya, Terukatsu Arima, Shin‐Ichi ‐I Akiyama

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78 Citations (Scopus)

Abstract

The overexpression of a cell‐surface glycoprotein termed P‐glycoprotein (P‐gp) is frequently associated with multidrug resistance (MDR) in cell lines in vitro. To evaluate the implications of P‐gp expression in clinical drug‐resistance, we examined the expression of P‐gp in fresh leukaemia cells from chronic myelogenous leukaemia (CML) patients in blast crisis. By using immunoblotting with a monoclonal antibody against P‐gp, C219, we showed that leukaemia cells from three CML patients in blast crisis were P‐gp negative at the stage when these patients were in complete remission, and that the cells showed high levels of P‐gp expression at times when the same patients had relapsed and had not responded to chemotherapy. Six out of 11 patients (nine in the refractory state) were P‐gp positive and they rarely responded to chemotherapy. These data suggest that the expression of P‐gp is closely associated with drug‐resistance in CML.

Original languageEnglish
Pages (from-to)24-29
Number of pages6
JournalBritish Journal of Haematology
Volume74
Issue number1
DOIs
Publication statusPublished - 1990 Jan
Externally publishedYes

ASJC Scopus subject areas

  • Hematology

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