Expression of the Musashi1 gene encoding the RNA-binding protein in human hepatoma cell lines

Hong Jin Shu, Takafumi Saito, Hisayoshi Watanabe, Jun Itu Ito, Hiroaki Takeda, Hideyuki Okano, Sumio Kawata

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Musashi1, a neural RNA-binding protein, plays an important role in regulating cell differentiation in precursor cells. Recently, expression of Musashi1 has been detected in human tumor tissues such as gliomas and melanomas, suggesting its involvement in oncogenic development. To determine any association between Musashi1 and the development of liver cancer, we investigated its gene expression in seven human hepatoma cell lines: HuH6, HuH7, Hep3B, SK-Hep1, HepG2, HLE, and HLF. Musashi1 mRNA expression was analyzed using the reverse-transcription polymerase chain reaction (PCR), and the PCR products were sequenced using a subcloning procedure. Musashi1 protein expression was analyzed in HuH7 and HepG2 cells by Western blot and immunofluorescence staining. Musashi1 mRNA was detected in the HuH6, HuH7, and Hep3B hepatoma cell lines, but not in the others. Sequencing of the PCR-amplified Musashi1 cDNA in these three cell lines showed the expected sequence of the human Musashi1 gene. Musashi1 protein expression was confirmed in HuH7 cells, which were positive for Musashi1 mRNA expression, but not in HepG2 cells. These results suggest that Musashi1 expression may be an important factor in the development of several types of carcinoma such as human hepatoma, and may be a useful molecular marker for tumor detection.

Original languageEnglish
Pages (from-to)150-154
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume293
Issue number1
DOIs
Publication statusPublished - 2002

Fingerprint

Gene encoding
RNA-Binding Proteins
Polymerase chain reaction
Hepatocellular Carcinoma
Cells
Gene Expression
Cell Line
Messenger RNA
Hep G2 Cells
Polymerase Chain Reaction
Transcription
Tumor Biomarkers
Reaction products
Gene expression
Liver
Tumors
Proteins
Liver Neoplasms
Complementary DNA
Genes

Keywords

  • Carcinogenesis
  • Hepatoma
  • Liver
  • Musashi
  • RNA-binding protein

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Expression of the Musashi1 gene encoding the RNA-binding protein in human hepatoma cell lines. / Shu, Hong Jin; Saito, Takafumi; Watanabe, Hisayoshi; Ito, Jun Itu; Takeda, Hiroaki; Okano, Hideyuki; Kawata, Sumio.

In: Biochemical and Biophysical Research Communications, Vol. 293, No. 1, 2002, p. 150-154.

Research output: Contribution to journalArticle

Shu, Hong Jin ; Saito, Takafumi ; Watanabe, Hisayoshi ; Ito, Jun Itu ; Takeda, Hiroaki ; Okano, Hideyuki ; Kawata, Sumio. / Expression of the Musashi1 gene encoding the RNA-binding protein in human hepatoma cell lines. In: Biochemical and Biophysical Research Communications. 2002 ; Vol. 293, No. 1. pp. 150-154.
@article{30ad55062a064f819110736aa85fe73a,
title = "Expression of the Musashi1 gene encoding the RNA-binding protein in human hepatoma cell lines",
abstract = "Musashi1, a neural RNA-binding protein, plays an important role in regulating cell differentiation in precursor cells. Recently, expression of Musashi1 has been detected in human tumor tissues such as gliomas and melanomas, suggesting its involvement in oncogenic development. To determine any association between Musashi1 and the development of liver cancer, we investigated its gene expression in seven human hepatoma cell lines: HuH6, HuH7, Hep3B, SK-Hep1, HepG2, HLE, and HLF. Musashi1 mRNA expression was analyzed using the reverse-transcription polymerase chain reaction (PCR), and the PCR products were sequenced using a subcloning procedure. Musashi1 protein expression was analyzed in HuH7 and HepG2 cells by Western blot and immunofluorescence staining. Musashi1 mRNA was detected in the HuH6, HuH7, and Hep3B hepatoma cell lines, but not in the others. Sequencing of the PCR-amplified Musashi1 cDNA in these three cell lines showed the expected sequence of the human Musashi1 gene. Musashi1 protein expression was confirmed in HuH7 cells, which were positive for Musashi1 mRNA expression, but not in HepG2 cells. These results suggest that Musashi1 expression may be an important factor in the development of several types of carcinoma such as human hepatoma, and may be a useful molecular marker for tumor detection.",
keywords = "Carcinogenesis, Hepatoma, Liver, Musashi, RNA-binding protein",
author = "Shu, {Hong Jin} and Takafumi Saito and Hisayoshi Watanabe and Ito, {Jun Itu} and Hiroaki Takeda and Hideyuki Okano and Sumio Kawata",
year = "2002",
doi = "10.1016/S0006-291X(02)00175-4",
language = "English",
volume = "293",
pages = "150--154",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Expression of the Musashi1 gene encoding the RNA-binding protein in human hepatoma cell lines

AU - Shu, Hong Jin

AU - Saito, Takafumi

AU - Watanabe, Hisayoshi

AU - Ito, Jun Itu

AU - Takeda, Hiroaki

AU - Okano, Hideyuki

AU - Kawata, Sumio

PY - 2002

Y1 - 2002

N2 - Musashi1, a neural RNA-binding protein, plays an important role in regulating cell differentiation in precursor cells. Recently, expression of Musashi1 has been detected in human tumor tissues such as gliomas and melanomas, suggesting its involvement in oncogenic development. To determine any association between Musashi1 and the development of liver cancer, we investigated its gene expression in seven human hepatoma cell lines: HuH6, HuH7, Hep3B, SK-Hep1, HepG2, HLE, and HLF. Musashi1 mRNA expression was analyzed using the reverse-transcription polymerase chain reaction (PCR), and the PCR products were sequenced using a subcloning procedure. Musashi1 protein expression was analyzed in HuH7 and HepG2 cells by Western blot and immunofluorescence staining. Musashi1 mRNA was detected in the HuH6, HuH7, and Hep3B hepatoma cell lines, but not in the others. Sequencing of the PCR-amplified Musashi1 cDNA in these three cell lines showed the expected sequence of the human Musashi1 gene. Musashi1 protein expression was confirmed in HuH7 cells, which were positive for Musashi1 mRNA expression, but not in HepG2 cells. These results suggest that Musashi1 expression may be an important factor in the development of several types of carcinoma such as human hepatoma, and may be a useful molecular marker for tumor detection.

AB - Musashi1, a neural RNA-binding protein, plays an important role in regulating cell differentiation in precursor cells. Recently, expression of Musashi1 has been detected in human tumor tissues such as gliomas and melanomas, suggesting its involvement in oncogenic development. To determine any association between Musashi1 and the development of liver cancer, we investigated its gene expression in seven human hepatoma cell lines: HuH6, HuH7, Hep3B, SK-Hep1, HepG2, HLE, and HLF. Musashi1 mRNA expression was analyzed using the reverse-transcription polymerase chain reaction (PCR), and the PCR products were sequenced using a subcloning procedure. Musashi1 protein expression was analyzed in HuH7 and HepG2 cells by Western blot and immunofluorescence staining. Musashi1 mRNA was detected in the HuH6, HuH7, and Hep3B hepatoma cell lines, but not in the others. Sequencing of the PCR-amplified Musashi1 cDNA in these three cell lines showed the expected sequence of the human Musashi1 gene. Musashi1 protein expression was confirmed in HuH7 cells, which were positive for Musashi1 mRNA expression, but not in HepG2 cells. These results suggest that Musashi1 expression may be an important factor in the development of several types of carcinoma such as human hepatoma, and may be a useful molecular marker for tumor detection.

KW - Carcinogenesis

KW - Hepatoma

KW - Liver

KW - Musashi

KW - RNA-binding protein

UR - http://www.scopus.com/inward/record.url?scp=0036290430&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036290430&partnerID=8YFLogxK

U2 - 10.1016/S0006-291X(02)00175-4

DO - 10.1016/S0006-291X(02)00175-4

M3 - Article

VL - 293

SP - 150

EP - 154

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 1

ER -