Expression of the neural RNA- binding protein Musashi1 in human gliomas

Masahiro Toda, Yukihiko Iizuka, Wangjie Yu, Takao Imai, Eiji Ikeda, Kazunari Yoshida, Takeshi Kawase, Yutaka Kawakami, Hideyuki Okano, Keiichi Uyemura

Research output: Contribution to journalArticle

125 Citations (Scopus)

Abstract

Tumor cells arising from a particular tissue may exhibit the same gene expression patterns as their precursor cells. To test this proposition, we have analyzed the expression of a neural RNA-binding protein, Musashi1, in primary human central nervous system (CNS) tumors. In rodents, Musashi1 is expressed predominantly in proliferating multipotent neural precursor cells, but not in newly generated postmitotic neurons. The expression of Musashi1 is downregulated with the successive progression of neurogenesis. In normal adult human tissues, we detected low levels of Musashi1 expression in brain and testis by RT-PCR analysis. In an RNA panel of 32 cancer tissues and cell lines, elevated expression of Musashi1 was seen in all five malignant gliomas studied, in contrast to the slight expression seen in other tumor cells, including those in several melanomas and a prostate cancer. Western blot analysis showed strong Musashi1 expression in malignant gliomas compared with nonneoplastic brain tissue. Glioblastomas, the most malignant form of glioma, showed higher Musashi1 expression than less malignant gliomas by immunohistochemical analysis. Tumors with strong Musashi1 expression tended to have high proliferative activity. Thus, the expression of Musashi1 correlated with the grade of the malignancy and proliferative activity in gliomas. These results suggest that primary CNS tumors may share gene expression patterns with primitive, undifferentiated CNS cells and that Musashi1 may be a useful marker for the diagnosis of CNS tumors.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalGLIA
Volume34
Issue number1
DOIs
Publication statusPublished - 2001

Fingerprint

RNA-Binding Proteins
Glioma
Central Nervous System Neoplasms
Neoplasms
Gene Expression
Neurogenesis
Brain
Glioblastoma
Testis
Rodentia
Melanoma
Prostatic Neoplasms
Down-Regulation
Central Nervous System
Western Blotting
RNA
Neurons
Cell Line
Polymerase Chain Reaction

Keywords

  • Brain tumor
  • Central nervous system
  • Expression
  • Malignancy
  • Proliferative activity

ASJC Scopus subject areas

  • Immunology

Cite this

Expression of the neural RNA- binding protein Musashi1 in human gliomas. / Toda, Masahiro; Iizuka, Yukihiko; Yu, Wangjie; Imai, Takao; Ikeda, Eiji; Yoshida, Kazunari; Kawase, Takeshi; Kawakami, Yutaka; Okano, Hideyuki; Uyemura, Keiichi.

In: GLIA, Vol. 34, No. 1, 2001, p. 1-7.

Research output: Contribution to journalArticle

Toda, M, Iizuka, Y, Yu, W, Imai, T, Ikeda, E, Yoshida, K, Kawase, T, Kawakami, Y, Okano, H & Uyemura, K 2001, 'Expression of the neural RNA- binding protein Musashi1 in human gliomas', GLIA, vol. 34, no. 1, pp. 1-7. https://doi.org/10.1002/glia.1034
Toda, Masahiro ; Iizuka, Yukihiko ; Yu, Wangjie ; Imai, Takao ; Ikeda, Eiji ; Yoshida, Kazunari ; Kawase, Takeshi ; Kawakami, Yutaka ; Okano, Hideyuki ; Uyemura, Keiichi. / Expression of the neural RNA- binding protein Musashi1 in human gliomas. In: GLIA. 2001 ; Vol. 34, No. 1. pp. 1-7.
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