Expression of the RET proto-oncogene in normal human tissues, pheochromocytomas, and other tumors of neural crest origin

K. Takaya, T. Yoshimasa, H. Arai, N. Tamura, Y. Miyamoto, Hiroshi Itoh, K. Nakao

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

In order to evaluate the roles of the RET proto-oncogene in normal human tissues and tumors derived from the neural crest cells, we examined the expression of RET in a variety of adult human tissues, pheochromocytomas, medullary thyroid carcinomas (MTCs), ganglioneuromas, and a neurinoma. Northern blot analysis demonstrated that RET is normally expressed in the adrenal medulla and cerebellum among adult human tissues. RET transcripts were detected in all of 11 sporadic and one familial pheochromocytomas. The levels of RET mRNA were higher in 8 of 12 pheochromocytomas than in the normal adrenal medulla, indicating that RET is overexpressed in the majority of sporadic pheochromocytomas. There was a considerable difference in the level of RET expression in each pheochromocytoma ranging from 0.2 to 10 times the transcripts found in the normal adrenal medulla. The sizes of the transcripts of 7.0, 6.0, 4.5, and 3.9 kb were the same as those found in the adrenal medulla, suggesting no rearrangements of the RET gene in pheochromocytomas. Southern blot analysis revealed neither amplification nor gross structural changes in the RET gene. RET was also expressed at high levels in four MTCs examined, whereas its transcripts were detected at low abundance in only one of three ganglioneuromas. RET was not expressed in a neurinoma. These results suggest that RET may play some roles in a limited range of adult human tissues, and that its high levels of expression may have relevance to development or growth of pheochromocytomas and MTCs.

Original languageEnglish
Pages (from-to)617-621
Number of pages5
JournalJournal of Molecular Medicine
Volume74
Issue number10
DOIs
Publication statusPublished - 1996
Externally publishedYes

Fingerprint

Proto-Oncogenes
Pheochromocytoma
Adrenal Medulla
Ganglioneuroma
Neurilemmoma
Gene Rearrangement
Neural Crest
Southern Blotting
Growth and Development
Northern Blotting
Cerebellum
Neural crest tumor
Messenger RNA
Genes
Medullary Thyroid cancer
Neoplasms

Keywords

  • Gene expression
  • Neural crest
  • Pheochromocytoma
  • Proto-oncogene
  • RET

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Expression of the RET proto-oncogene in normal human tissues, pheochromocytomas, and other tumors of neural crest origin. / Takaya, K.; Yoshimasa, T.; Arai, H.; Tamura, N.; Miyamoto, Y.; Itoh, Hiroshi; Nakao, K.

In: Journal of Molecular Medicine, Vol. 74, No. 10, 1996, p. 617-621.

Research output: Contribution to journalArticle

Takaya, K. ; Yoshimasa, T. ; Arai, H. ; Tamura, N. ; Miyamoto, Y. ; Itoh, Hiroshi ; Nakao, K. / Expression of the RET proto-oncogene in normal human tissues, pheochromocytomas, and other tumors of neural crest origin. In: Journal of Molecular Medicine. 1996 ; Vol. 74, No. 10. pp. 617-621.
@article{86f03a70641646b7a0357f7567289467,
title = "Expression of the RET proto-oncogene in normal human tissues, pheochromocytomas, and other tumors of neural crest origin",
abstract = "In order to evaluate the roles of the RET proto-oncogene in normal human tissues and tumors derived from the neural crest cells, we examined the expression of RET in a variety of adult human tissues, pheochromocytomas, medullary thyroid carcinomas (MTCs), ganglioneuromas, and a neurinoma. Northern blot analysis demonstrated that RET is normally expressed in the adrenal medulla and cerebellum among adult human tissues. RET transcripts were detected in all of 11 sporadic and one familial pheochromocytomas. The levels of RET mRNA were higher in 8 of 12 pheochromocytomas than in the normal adrenal medulla, indicating that RET is overexpressed in the majority of sporadic pheochromocytomas. There was a considerable difference in the level of RET expression in each pheochromocytoma ranging from 0.2 to 10 times the transcripts found in the normal adrenal medulla. The sizes of the transcripts of 7.0, 6.0, 4.5, and 3.9 kb were the same as those found in the adrenal medulla, suggesting no rearrangements of the RET gene in pheochromocytomas. Southern blot analysis revealed neither amplification nor gross structural changes in the RET gene. RET was also expressed at high levels in four MTCs examined, whereas its transcripts were detected at low abundance in only one of three ganglioneuromas. RET was not expressed in a neurinoma. These results suggest that RET may play some roles in a limited range of adult human tissues, and that its high levels of expression may have relevance to development or growth of pheochromocytomas and MTCs.",
keywords = "Gene expression, Neural crest, Pheochromocytoma, Proto-oncogene, RET",
author = "K. Takaya and T. Yoshimasa and H. Arai and N. Tamura and Y. Miyamoto and Hiroshi Itoh and K. Nakao",
year = "1996",
doi = "10.1007/s001090050065",
language = "English",
volume = "74",
pages = "617--621",
journal = "Journal of Molecular Medicine",
issn = "0946-2716",
publisher = "Springer Verlag",
number = "10",

}

TY - JOUR

T1 - Expression of the RET proto-oncogene in normal human tissues, pheochromocytomas, and other tumors of neural crest origin

AU - Takaya, K.

AU - Yoshimasa, T.

AU - Arai, H.

AU - Tamura, N.

AU - Miyamoto, Y.

AU - Itoh, Hiroshi

AU - Nakao, K.

PY - 1996

Y1 - 1996

N2 - In order to evaluate the roles of the RET proto-oncogene in normal human tissues and tumors derived from the neural crest cells, we examined the expression of RET in a variety of adult human tissues, pheochromocytomas, medullary thyroid carcinomas (MTCs), ganglioneuromas, and a neurinoma. Northern blot analysis demonstrated that RET is normally expressed in the adrenal medulla and cerebellum among adult human tissues. RET transcripts were detected in all of 11 sporadic and one familial pheochromocytomas. The levels of RET mRNA were higher in 8 of 12 pheochromocytomas than in the normal adrenal medulla, indicating that RET is overexpressed in the majority of sporadic pheochromocytomas. There was a considerable difference in the level of RET expression in each pheochromocytoma ranging from 0.2 to 10 times the transcripts found in the normal adrenal medulla. The sizes of the transcripts of 7.0, 6.0, 4.5, and 3.9 kb were the same as those found in the adrenal medulla, suggesting no rearrangements of the RET gene in pheochromocytomas. Southern blot analysis revealed neither amplification nor gross structural changes in the RET gene. RET was also expressed at high levels in four MTCs examined, whereas its transcripts were detected at low abundance in only one of three ganglioneuromas. RET was not expressed in a neurinoma. These results suggest that RET may play some roles in a limited range of adult human tissues, and that its high levels of expression may have relevance to development or growth of pheochromocytomas and MTCs.

AB - In order to evaluate the roles of the RET proto-oncogene in normal human tissues and tumors derived from the neural crest cells, we examined the expression of RET in a variety of adult human tissues, pheochromocytomas, medullary thyroid carcinomas (MTCs), ganglioneuromas, and a neurinoma. Northern blot analysis demonstrated that RET is normally expressed in the adrenal medulla and cerebellum among adult human tissues. RET transcripts were detected in all of 11 sporadic and one familial pheochromocytomas. The levels of RET mRNA were higher in 8 of 12 pheochromocytomas than in the normal adrenal medulla, indicating that RET is overexpressed in the majority of sporadic pheochromocytomas. There was a considerable difference in the level of RET expression in each pheochromocytoma ranging from 0.2 to 10 times the transcripts found in the normal adrenal medulla. The sizes of the transcripts of 7.0, 6.0, 4.5, and 3.9 kb were the same as those found in the adrenal medulla, suggesting no rearrangements of the RET gene in pheochromocytomas. Southern blot analysis revealed neither amplification nor gross structural changes in the RET gene. RET was also expressed at high levels in four MTCs examined, whereas its transcripts were detected at low abundance in only one of three ganglioneuromas. RET was not expressed in a neurinoma. These results suggest that RET may play some roles in a limited range of adult human tissues, and that its high levels of expression may have relevance to development or growth of pheochromocytomas and MTCs.

KW - Gene expression

KW - Neural crest

KW - Pheochromocytoma

KW - Proto-oncogene

KW - RET

UR - http://www.scopus.com/inward/record.url?scp=0029851837&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029851837&partnerID=8YFLogxK

U2 - 10.1007/s001090050065

DO - 10.1007/s001090050065

M3 - Article

VL - 74

SP - 617

EP - 621

JO - Journal of Molecular Medicine

JF - Journal of Molecular Medicine

SN - 0946-2716

IS - 10

ER -