Expression of trophinin, tastin, and bystin by trophoblast and endometrial cells in human placenta

Nao Suzuki, Jun Nakayama, Ie Ming Shih, Daisuke Aoki, Shiro Nozawa, Michiko N. Fukuda

Research output: Contribution to journalArticle

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Abstract

Trophinin, tastin, and bystin comprise a complex mediating a unique homophilic cell adhesion between trophoblast and endometrial epithelial cells at their respective apical cell surfaces. In this study, we prepared mouse monoclonal antibodies specific to each of these molecules. The expression of these molecules in the human placenta was examined immunohistochemically using the antibodies. In placenta from the 6th week of pregnancy, trophinin and bystin were found in the cytoplasm of the syncytiotrophoblast in the chorionic villi, and in endometrial decidual cells at the utero placental interface. Tastin was exclusively present on the apical side of the syncytiotrophoblast. Tissue sections were also examined by in situ hybridization using RNA probes specific to each of these molecules. This analysis showed that trophoblast and endometrial epithelial cells at the utero placental interface express trophinin, tastin, and bystin. In wk 10 placenta, trophinin and bystin were found in the intravillous cytotrophoblast, while tastin was not found in the villi. After wk 10, levels of all three proteins decreased and then disappeared from placental villi.

Original languageEnglish
Pages (from-to)621-627
Number of pages7
JournalBiology of Reproduction
Volume60
Issue number3
Publication statusPublished - 1999

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Trophoblasts
Placenta
Chorionic Villi
Epithelial Cells
RNA Probes
Cell Adhesion
In Situ Hybridization
Cytoplasm
Monoclonal Antibodies
Pregnancy
Antibodies
Proteins

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

Suzuki, N., Nakayama, J., Shih, I. M., Aoki, D., Nozawa, S., & Fukuda, M. N. (1999). Expression of trophinin, tastin, and bystin by trophoblast and endometrial cells in human placenta. Biology of Reproduction, 60(3), 621-627.

Expression of trophinin, tastin, and bystin by trophoblast and endometrial cells in human placenta. / Suzuki, Nao; Nakayama, Jun; Shih, Ie Ming; Aoki, Daisuke; Nozawa, Shiro; Fukuda, Michiko N.

In: Biology of Reproduction, Vol. 60, No. 3, 1999, p. 621-627.

Research output: Contribution to journalArticle

Suzuki, N, Nakayama, J, Shih, IM, Aoki, D, Nozawa, S & Fukuda, MN 1999, 'Expression of trophinin, tastin, and bystin by trophoblast and endometrial cells in human placenta', Biology of Reproduction, vol. 60, no. 3, pp. 621-627.
Suzuki, Nao ; Nakayama, Jun ; Shih, Ie Ming ; Aoki, Daisuke ; Nozawa, Shiro ; Fukuda, Michiko N. / Expression of trophinin, tastin, and bystin by trophoblast and endometrial cells in human placenta. In: Biology of Reproduction. 1999 ; Vol. 60, No. 3. pp. 621-627.
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