Expression profiling of sulfotransferases in human cell lines derived from extra-hepatic tissues

H. O. Tamura, K. Taniguchi, E. Havashi, Y. Hiyoshi, F. Nagai

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Abstract

To explore the physiological roles of sulfotransferases (SULTs) in extra-hepatic tissues, we examined the expression of eight SULT genes by reverse transcription (RT)-PCR in human cell lines that were established from various tissues. Expression levels of SULTs were low in neural cell lines such as NB-1 and GI-1, and high in epithelial cell lines, such as Caco-2 and BeWo. SULT1C2 expression was abundant in all cell types, whereas that of SULT1E1, SULT1B1 or SULT2B1 was restricted to a specific cell type. SULT1C1, which can catalyze the sulfation of N-hydroxy-2-acetylaminofluorence, was expressed in Caco-2, BeWo and KB562. Induction of differentiation did not generally affect SULT expression, although that of SULT1C2 was reduced after differentiation of the neuroblastoma cell line, NB-1, was induced. The profile of SULT expression in the culture cells obtained here gives clues to understanding the physiological roles of SULT enzymes in extra-hepatic tissues or organs.

Original languageEnglish
Pages (from-to)1258-1262
Number of pages5
JournalBiological and Pharmaceutical Bulletin
Volume24
Issue number11
DOIs
Publication statusPublished - 2001 Nov 21

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Keywords

  • Caco-2
  • Culture cell
  • Expression
  • Sulfotransferase

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

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