Expression profiling of sulfotransferases in human cell lines derived from extra-hepatic tissues

Hiroomi Tamura, K. Taniguchi, E. Havashi, Y. Hiyoshi, F. Nagai

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

To explore the physiological roles of sulfotransferases (SULTs) in extra-hepatic tissues, we examined the expression of eight SULT genes by reverse transcription (RT)-PCR in human cell lines that were established from various tissues. Expression levels of SULTs were low in neural cell lines such as NB-1 and GI-1, and high in epithelial cell lines, such as Caco-2 and BeWo. SULT1C2 expression was abundant in all cell types, whereas that of SULT1E1, SULT1B1 or SULT2B1 was restricted to a specific cell type. SULT1C1, which can catalyze the sulfation of N-hydroxy-2-acetylaminofluorence, was expressed in Caco-2, BeWo and KB562. Induction of differentiation did not generally affect SULT expression, although that of SULT1C2 was reduced after differentiation of the neuroblastoma cell line, NB-1, was induced. The profile of SULT expression in the culture cells obtained here gives clues to understanding the physiological roles of SULT enzymes in extra-hepatic tissues or organs.

Original languageEnglish
Pages (from-to)1258-1262
Number of pages5
JournalBiological and Pharmaceutical Bulletin
Volume24
Issue number11
DOIs
Publication statusPublished - 2001

Fingerprint

Sulfotransferases
Cell Line
Liver
Neuroblastoma
Reverse Transcription
Cell Culture Techniques
Epithelial Cells
Polymerase Chain Reaction
Enzymes
Genes

Keywords

  • Caco-2
  • Culture cell
  • Expression
  • Sulfotransferase

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Expression profiling of sulfotransferases in human cell lines derived from extra-hepatic tissues. / Tamura, Hiroomi; Taniguchi, K.; Havashi, E.; Hiyoshi, Y.; Nagai, F.

In: Biological and Pharmaceutical Bulletin, Vol. 24, No. 11, 2001, p. 1258-1262.

Research output: Contribution to journalArticle

Tamura, Hiroomi ; Taniguchi, K. ; Havashi, E. ; Hiyoshi, Y. ; Nagai, F. / Expression profiling of sulfotransferases in human cell lines derived from extra-hepatic tissues. In: Biological and Pharmaceutical Bulletin. 2001 ; Vol. 24, No. 11. pp. 1258-1262.
@article{fbaf9fbe82b440609a101c0d599270a5,
title = "Expression profiling of sulfotransferases in human cell lines derived from extra-hepatic tissues",
abstract = "To explore the physiological roles of sulfotransferases (SULTs) in extra-hepatic tissues, we examined the expression of eight SULT genes by reverse transcription (RT)-PCR in human cell lines that were established from various tissues. Expression levels of SULTs were low in neural cell lines such as NB-1 and GI-1, and high in epithelial cell lines, such as Caco-2 and BeWo. SULT1C2 expression was abundant in all cell types, whereas that of SULT1E1, SULT1B1 or SULT2B1 was restricted to a specific cell type. SULT1C1, which can catalyze the sulfation of N-hydroxy-2-acetylaminofluorence, was expressed in Caco-2, BeWo and KB562. Induction of differentiation did not generally affect SULT expression, although that of SULT1C2 was reduced after differentiation of the neuroblastoma cell line, NB-1, was induced. The profile of SULT expression in the culture cells obtained here gives clues to understanding the physiological roles of SULT enzymes in extra-hepatic tissues or organs.",
keywords = "Caco-2, Culture cell, Expression, Sulfotransferase",
author = "Hiroomi Tamura and K. Taniguchi and E. Havashi and Y. Hiyoshi and F. Nagai",
year = "2001",
doi = "10.1248/bpb.24.1258",
language = "English",
volume = "24",
pages = "1258--1262",
journal = "Biological and Pharmaceutical Bulletin",
issn = "0918-6158",
publisher = "Pharmaceutical Society of Japan",
number = "11",

}

TY - JOUR

T1 - Expression profiling of sulfotransferases in human cell lines derived from extra-hepatic tissues

AU - Tamura, Hiroomi

AU - Taniguchi, K.

AU - Havashi, E.

AU - Hiyoshi, Y.

AU - Nagai, F.

PY - 2001

Y1 - 2001

N2 - To explore the physiological roles of sulfotransferases (SULTs) in extra-hepatic tissues, we examined the expression of eight SULT genes by reverse transcription (RT)-PCR in human cell lines that were established from various tissues. Expression levels of SULTs were low in neural cell lines such as NB-1 and GI-1, and high in epithelial cell lines, such as Caco-2 and BeWo. SULT1C2 expression was abundant in all cell types, whereas that of SULT1E1, SULT1B1 or SULT2B1 was restricted to a specific cell type. SULT1C1, which can catalyze the sulfation of N-hydroxy-2-acetylaminofluorence, was expressed in Caco-2, BeWo and KB562. Induction of differentiation did not generally affect SULT expression, although that of SULT1C2 was reduced after differentiation of the neuroblastoma cell line, NB-1, was induced. The profile of SULT expression in the culture cells obtained here gives clues to understanding the physiological roles of SULT enzymes in extra-hepatic tissues or organs.

AB - To explore the physiological roles of sulfotransferases (SULTs) in extra-hepatic tissues, we examined the expression of eight SULT genes by reverse transcription (RT)-PCR in human cell lines that were established from various tissues. Expression levels of SULTs were low in neural cell lines such as NB-1 and GI-1, and high in epithelial cell lines, such as Caco-2 and BeWo. SULT1C2 expression was abundant in all cell types, whereas that of SULT1E1, SULT1B1 or SULT2B1 was restricted to a specific cell type. SULT1C1, which can catalyze the sulfation of N-hydroxy-2-acetylaminofluorence, was expressed in Caco-2, BeWo and KB562. Induction of differentiation did not generally affect SULT expression, although that of SULT1C2 was reduced after differentiation of the neuroblastoma cell line, NB-1, was induced. The profile of SULT expression in the culture cells obtained here gives clues to understanding the physiological roles of SULT enzymes in extra-hepatic tissues or organs.

KW - Caco-2

KW - Culture cell

KW - Expression

KW - Sulfotransferase

UR - http://www.scopus.com/inward/record.url?scp=0035158397&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035158397&partnerID=8YFLogxK

U2 - 10.1248/bpb.24.1258

DO - 10.1248/bpb.24.1258

M3 - Article

VL - 24

SP - 1258

EP - 1262

JO - Biological and Pharmaceutical Bulletin

JF - Biological and Pharmaceutical Bulletin

SN - 0918-6158

IS - 11

ER -