Expression status of RUNX1/AML1 in normal gastric epithelium and its mutational analysis in microdissected gastric cancer cells

Takebumi Usui, Kazuhiko Aoyagi, Norihisa Saeki, Yukihiro Nakanishi, Yae Kanai, Misao Ohki, Kenji Ogawa, Teruhiko Yoshida, Hiroki Sasaki

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Although Runt-related transcription factors RUNXs (RUNX1-3) have a high similarity in their structure, only RUNX3 is known to be involved in gastric carcinogenesis. First, we examined mRNA expression of these three RUNX genes in the gastric mucosa, and, finding only RUNX2 was not expressed there, we further investigated RUNX1 and RUNX3 expression in three regions including the pit, isthmus/neck, and gland regions of the human normal stomach and whether RUNX1 is involved in gastric carcinogenesis. The mRNA expression of RUNX1 and RUNX3 was examined by use of the three regions isolated by laser-captured microdissection (LCM) and by use of primary gastric cancer tissues. Furthermore, RUNX1 mutational analysis was performed in the cancer cells, which also were isolated from 44 paraffin-embedded gastric cancer tissues by LCM. RUNX1 was coexpressed with RUNX3 in the pit region, and has cell growth-inhibition activity similar to RUNX3. RUNX3 has been reported to be suppressed by DNA methylation in a subset of gastric cancers; however, the expression of RUNX1 mRNA was observed in all of the gastric cancer cell lines and gastric cancer tissues that we examined. No RUNX1 mutation was found in the 44 gastric cancer patients. Although RUNX1 is similar to RUNX3 in both the expression pattern in the stomach and its cell growth-inhibition activity, RUNX1 is not involved in most cases of gastric cancers. These results suggest that the transcriptional target genes are different between these two family genes.

Original languageEnglish
Pages (from-to)779-784
Number of pages6
JournalInternational Journal of Oncology
Volume29
Issue number4
Publication statusPublished - 2006 Oct
Externally publishedYes

Fingerprint

Stomach Neoplasms
Stomach
Epithelium
Microdissection
Messenger RNA
Carcinogenesis
Lasers
Genes
DNA Methylation
Growth
Gastric Mucosa
Paraffin
Transcription Factors
Neck
Cell Line
Mutation
Neoplasms

Keywords

  • Gastric cancer
  • Microdissection
  • RUNX1
  • RUNX3
  • Stomach
  • Tumor suppressor gene

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Expression status of RUNX1/AML1 in normal gastric epithelium and its mutational analysis in microdissected gastric cancer cells. / Usui, Takebumi; Aoyagi, Kazuhiko; Saeki, Norihisa; Nakanishi, Yukihiro; Kanai, Yae; Ohki, Misao; Ogawa, Kenji; Yoshida, Teruhiko; Sasaki, Hiroki.

In: International Journal of Oncology, Vol. 29, No. 4, 10.2006, p. 779-784.

Research output: Contribution to journalArticle

Usui, T, Aoyagi, K, Saeki, N, Nakanishi, Y, Kanai, Y, Ohki, M, Ogawa, K, Yoshida, T & Sasaki, H 2006, 'Expression status of RUNX1/AML1 in normal gastric epithelium and its mutational analysis in microdissected gastric cancer cells', International Journal of Oncology, vol. 29, no. 4, pp. 779-784.
Usui, Takebumi ; Aoyagi, Kazuhiko ; Saeki, Norihisa ; Nakanishi, Yukihiro ; Kanai, Yae ; Ohki, Misao ; Ogawa, Kenji ; Yoshida, Teruhiko ; Sasaki, Hiroki. / Expression status of RUNX1/AML1 in normal gastric epithelium and its mutational analysis in microdissected gastric cancer cells. In: International Journal of Oncology. 2006 ; Vol. 29, No. 4. pp. 779-784.
@article{5053d8d406d74678a367ec84bca9f909,
title = "Expression status of RUNX1/AML1 in normal gastric epithelium and its mutational analysis in microdissected gastric cancer cells",
abstract = "Although Runt-related transcription factors RUNXs (RUNX1-3) have a high similarity in their structure, only RUNX3 is known to be involved in gastric carcinogenesis. First, we examined mRNA expression of these three RUNX genes in the gastric mucosa, and, finding only RUNX2 was not expressed there, we further investigated RUNX1 and RUNX3 expression in three regions including the pit, isthmus/neck, and gland regions of the human normal stomach and whether RUNX1 is involved in gastric carcinogenesis. The mRNA expression of RUNX1 and RUNX3 was examined by use of the three regions isolated by laser-captured microdissection (LCM) and by use of primary gastric cancer tissues. Furthermore, RUNX1 mutational analysis was performed in the cancer cells, which also were isolated from 44 paraffin-embedded gastric cancer tissues by LCM. RUNX1 was coexpressed with RUNX3 in the pit region, and has cell growth-inhibition activity similar to RUNX3. RUNX3 has been reported to be suppressed by DNA methylation in a subset of gastric cancers; however, the expression of RUNX1 mRNA was observed in all of the gastric cancer cell lines and gastric cancer tissues that we examined. No RUNX1 mutation was found in the 44 gastric cancer patients. Although RUNX1 is similar to RUNX3 in both the expression pattern in the stomach and its cell growth-inhibition activity, RUNX1 is not involved in most cases of gastric cancers. These results suggest that the transcriptional target genes are different between these two family genes.",
keywords = "Gastric cancer, Microdissection, RUNX1, RUNX3, Stomach, Tumor suppressor gene",
author = "Takebumi Usui and Kazuhiko Aoyagi and Norihisa Saeki and Yukihiro Nakanishi and Yae Kanai and Misao Ohki and Kenji Ogawa and Teruhiko Yoshida and Hiroki Sasaki",
year = "2006",
month = "10",
language = "English",
volume = "29",
pages = "779--784",
journal = "International Journal of Oncology",
issn = "1019-6439",
publisher = "Spandidos Publications",
number = "4",

}

TY - JOUR

T1 - Expression status of RUNX1/AML1 in normal gastric epithelium and its mutational analysis in microdissected gastric cancer cells

AU - Usui, Takebumi

AU - Aoyagi, Kazuhiko

AU - Saeki, Norihisa

AU - Nakanishi, Yukihiro

AU - Kanai, Yae

AU - Ohki, Misao

AU - Ogawa, Kenji

AU - Yoshida, Teruhiko

AU - Sasaki, Hiroki

PY - 2006/10

Y1 - 2006/10

N2 - Although Runt-related transcription factors RUNXs (RUNX1-3) have a high similarity in their structure, only RUNX3 is known to be involved in gastric carcinogenesis. First, we examined mRNA expression of these three RUNX genes in the gastric mucosa, and, finding only RUNX2 was not expressed there, we further investigated RUNX1 and RUNX3 expression in three regions including the pit, isthmus/neck, and gland regions of the human normal stomach and whether RUNX1 is involved in gastric carcinogenesis. The mRNA expression of RUNX1 and RUNX3 was examined by use of the three regions isolated by laser-captured microdissection (LCM) and by use of primary gastric cancer tissues. Furthermore, RUNX1 mutational analysis was performed in the cancer cells, which also were isolated from 44 paraffin-embedded gastric cancer tissues by LCM. RUNX1 was coexpressed with RUNX3 in the pit region, and has cell growth-inhibition activity similar to RUNX3. RUNX3 has been reported to be suppressed by DNA methylation in a subset of gastric cancers; however, the expression of RUNX1 mRNA was observed in all of the gastric cancer cell lines and gastric cancer tissues that we examined. No RUNX1 mutation was found in the 44 gastric cancer patients. Although RUNX1 is similar to RUNX3 in both the expression pattern in the stomach and its cell growth-inhibition activity, RUNX1 is not involved in most cases of gastric cancers. These results suggest that the transcriptional target genes are different between these two family genes.

AB - Although Runt-related transcription factors RUNXs (RUNX1-3) have a high similarity in their structure, only RUNX3 is known to be involved in gastric carcinogenesis. First, we examined mRNA expression of these three RUNX genes in the gastric mucosa, and, finding only RUNX2 was not expressed there, we further investigated RUNX1 and RUNX3 expression in three regions including the pit, isthmus/neck, and gland regions of the human normal stomach and whether RUNX1 is involved in gastric carcinogenesis. The mRNA expression of RUNX1 and RUNX3 was examined by use of the three regions isolated by laser-captured microdissection (LCM) and by use of primary gastric cancer tissues. Furthermore, RUNX1 mutational analysis was performed in the cancer cells, which also were isolated from 44 paraffin-embedded gastric cancer tissues by LCM. RUNX1 was coexpressed with RUNX3 in the pit region, and has cell growth-inhibition activity similar to RUNX3. RUNX3 has been reported to be suppressed by DNA methylation in a subset of gastric cancers; however, the expression of RUNX1 mRNA was observed in all of the gastric cancer cell lines and gastric cancer tissues that we examined. No RUNX1 mutation was found in the 44 gastric cancer patients. Although RUNX1 is similar to RUNX3 in both the expression pattern in the stomach and its cell growth-inhibition activity, RUNX1 is not involved in most cases of gastric cancers. These results suggest that the transcriptional target genes are different between these two family genes.

KW - Gastric cancer

KW - Microdissection

KW - RUNX1

KW - RUNX3

KW - Stomach

KW - Tumor suppressor gene

UR - http://www.scopus.com/inward/record.url?scp=39049186567&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=39049186567&partnerID=8YFLogxK

M3 - Article

C2 - 16964375

AN - SCOPUS:39049186567

VL - 29

SP - 779

EP - 784

JO - International Journal of Oncology

JF - International Journal of Oncology

SN - 1019-6439

IS - 4

ER -