Abstract
Purpose. We reported that extended-wear soft contact lens (EWSCL) wearer's human superficial corneal epithelial cell size were larger than normal's. But this mechanism is unknown. We think the mechanism may be produced by decreasing an impulse to epithelial cells by blinking or EWSCL wearer inhibiting a corneal epithelium apoptosis. In this study we analyzed EWSCL wearers and normal rabbit corneal apoptosis. Methods. EWSCL was placed on white rabbit eye for one month. The other eye was used as the normal control. We analyzed superficial corneal epithelial cell size, using specular microscope. Apoptosis was detected by using the APOPTAG™ (ONCOR Co LTD.), apoptosis detection kit. Results. The initial superficial corneal epithelial cell size of 570±251 μm2 changed after one month 945±370 μm2. Staining, which indicates DNA fragmentation, was detected in only the upper 2 to 3 layers of the corneal epithelium. Stronger staining was observed in the normal control corneal epithelium. Conclusions. EWSCL wearer's corneal epithelial cell size increased and apoptosis cells decreased. These findings indicate that EWSCL wear may inhibit the programmed cell death of the corneal epithelium.
| Original language | English |
|---|---|
| Journal | Investigative Ophthalmology and Visual Science |
| Volume | 37 |
| Issue number | 3 |
| Publication status | Published - 1996 Feb 15 |
| Externally published | Yes |
Fingerprint
ASJC Scopus subject areas
- Ophthalmology
Cite this
Extended-wear soft contact lens and corneal epithelium apoptosis. / Hata, S.; Urayama, K.; Shinozaki, N.; Fukuda, M.; Tsubota, Kazuo.
In: Investigative Ophthalmology and Visual Science, Vol. 37, No. 3, 15.02.1996.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Extended-wear soft contact lens and corneal epithelium apoptosis
AU - Hata, S.
AU - Urayama, K.
AU - Shinozaki, N.
AU - Fukuda, M.
AU - Tsubota, Kazuo
PY - 1996/2/15
Y1 - 1996/2/15
N2 - Purpose. We reported that extended-wear soft contact lens (EWSCL) wearer's human superficial corneal epithelial cell size were larger than normal's. But this mechanism is unknown. We think the mechanism may be produced by decreasing an impulse to epithelial cells by blinking or EWSCL wearer inhibiting a corneal epithelium apoptosis. In this study we analyzed EWSCL wearers and normal rabbit corneal apoptosis. Methods. EWSCL was placed on white rabbit eye for one month. The other eye was used as the normal control. We analyzed superficial corneal epithelial cell size, using specular microscope. Apoptosis was detected by using the APOPTAG™ (ONCOR Co LTD.), apoptosis detection kit. Results. The initial superficial corneal epithelial cell size of 570±251 μm2 changed after one month 945±370 μm2. Staining, which indicates DNA fragmentation, was detected in only the upper 2 to 3 layers of the corneal epithelium. Stronger staining was observed in the normal control corneal epithelium. Conclusions. EWSCL wearer's corneal epithelial cell size increased and apoptosis cells decreased. These findings indicate that EWSCL wear may inhibit the programmed cell death of the corneal epithelium.
AB - Purpose. We reported that extended-wear soft contact lens (EWSCL) wearer's human superficial corneal epithelial cell size were larger than normal's. But this mechanism is unknown. We think the mechanism may be produced by decreasing an impulse to epithelial cells by blinking or EWSCL wearer inhibiting a corneal epithelium apoptosis. In this study we analyzed EWSCL wearers and normal rabbit corneal apoptosis. Methods. EWSCL was placed on white rabbit eye for one month. The other eye was used as the normal control. We analyzed superficial corneal epithelial cell size, using specular microscope. Apoptosis was detected by using the APOPTAG™ (ONCOR Co LTD.), apoptosis detection kit. Results. The initial superficial corneal epithelial cell size of 570±251 μm2 changed after one month 945±370 μm2. Staining, which indicates DNA fragmentation, was detected in only the upper 2 to 3 layers of the corneal epithelium. Stronger staining was observed in the normal control corneal epithelium. Conclusions. EWSCL wearer's corneal epithelial cell size increased and apoptosis cells decreased. These findings indicate that EWSCL wear may inhibit the programmed cell death of the corneal epithelium.
UR - http://www.scopus.com/inward/record.url?scp=33750173009&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33750173009&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:33750173009
VL - 37
JO - Investigative Ophthalmology and Visual Science
JF - Investigative Ophthalmology and Visual Science
SN - 0146-0404
IS - 3
ER -