Extensive RNA editing in transcripts from the PsbB operon and RpoA gene of plastids from the enigmatic moss Takakia lepidozioides

Mamoru Sugita, Yuki Miyata, Kaori Maruyama, Chika Sugiura, Tomotsugu Arikawa, Masanobu Higuchi

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

RNA editing is a post-transcriptional process that changes individual nucleotides in transcripts, and usually occurs in the plastids of land plants. The number of RNA editing sites in a plastid is significantly divergent in bryophytes, ranging from zero in liverworts to almost 1,000 sites in hornworts. In this study, we identified 132 RNA editing sites in the transcripts of six genes from the psbB operon and the rpoA of the moss Takakia lepidozioides. This is the highest number of RNA editing sites known in this region among land plant species. All were cytidine-to-uridine conversions. More than 91% of RNA editing occurred at the first or second codon positions, and it altered amino acid identity. Six editing sites created new translation initiation codons or stop codons. Thirty-two sites were commonly observed in the hornwort Anthoceros angustus. This finding suggests that the enigmatic bryophyte Takakia is closely related to hornworts with respect to RNA editing events.

Original languageEnglish
Pages (from-to)2268-2274
Number of pages7
JournalBioscience, Biotechnology and Biochemistry
Volume70
Issue number9
DOIs
Publication statusPublished - 2006

Fingerprint

RNA Editing
RNA editing
Bryophyta
Plastids
Anthocerotophyta
operon
Operon
RNA
plastids
mosses and liverworts
Genes
Embryophyta
genes
embryophytes
Hepatophyta
cytidine
Cytidine
start codon
Initiator Codon
uridine

Keywords

  • Cytidine-to-uridine conversion
  • Moss
  • Plastid
  • RNA editing
  • Takakia lepidozioides

ASJC Scopus subject areas

  • Bioengineering
  • Biotechnology
  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry (miscellaneous)
  • Applied Microbiology and Biotechnology
  • Food Science

Cite this

Extensive RNA editing in transcripts from the PsbB operon and RpoA gene of plastids from the enigmatic moss Takakia lepidozioides. / Sugita, Mamoru; Miyata, Yuki; Maruyama, Kaori; Sugiura, Chika; Arikawa, Tomotsugu; Higuchi, Masanobu.

In: Bioscience, Biotechnology and Biochemistry, Vol. 70, No. 9, 2006, p. 2268-2274.

Research output: Contribution to journalArticle

Sugita, Mamoru ; Miyata, Yuki ; Maruyama, Kaori ; Sugiura, Chika ; Arikawa, Tomotsugu ; Higuchi, Masanobu. / Extensive RNA editing in transcripts from the PsbB operon and RpoA gene of plastids from the enigmatic moss Takakia lepidozioides. In: Bioscience, Biotechnology and Biochemistry. 2006 ; Vol. 70, No. 9. pp. 2268-2274.
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