External negative electric potential accelerates exocytosis of lamellar bodies in human skin ex vivo

Junichi Kumamoto, Makiko Goto, Sumiko Denda, Masashi Nakatani, Yuya Takasugi, Katsunori Tsuchiya, Yuji Shimizu, Yusuke Takatsuru, Mitsuhiro Denda

Research output: Contribution to journalLetter

3 Citations (Scopus)

Abstract

Exocytosis of lamellar bodies at the uppermost nucleated layer of the epidermis is a crucial process for epidermal permeability barrier homoeostasis. We have previously suggested that skin surface electric potential might be associated with barrier homoeostasis. Thus, we hypothesized that the potential might drive exocytosis of lamellar bodies. In this study, we tested this idea by applying negative electric potential (-0.5 V) to human skin samples ex vivo for 2 h and observing the ultrastructure of the uppermost layer. The secretion of lamellar bodies was accelerated in the potential-applied skin, compared to that in untreated control skin. Multiphoton observation indicated that extracellular lipid domains were more extensive in treated skin than in control skin. Moreover, the calcium ion gradient was greater at the uppermost layer of the epidermis of treated skin, compared to that in control skin. These results indicate that electric potential may regulate lamellar body secretion in healthy human skin.

Original languageEnglish
Pages (from-to)421-423
Number of pages3
JournalExperimental Dermatology
Volume22
Issue number6
DOIs
Publication statusPublished - 2013 Jun
Externally publishedYes

Fingerprint

Exocytosis
Human Body
Skin
Electric potential
Epidermis
Homeostasis
Permeability
Observation
Ions
Calcium
Lipids

Keywords

  • Barrier
  • Calcium
  • Epidermis
  • Keratinocyte
  • Multi-photon

ASJC Scopus subject areas

  • Dermatology
  • Molecular Biology
  • Biochemistry

Cite this

External negative electric potential accelerates exocytosis of lamellar bodies in human skin ex vivo. / Kumamoto, Junichi; Goto, Makiko; Denda, Sumiko; Nakatani, Masashi; Takasugi, Yuya; Tsuchiya, Katsunori; Shimizu, Yuji; Takatsuru, Yusuke; Denda, Mitsuhiro.

In: Experimental Dermatology, Vol. 22, No. 6, 06.2013, p. 421-423.

Research output: Contribution to journalLetter

Kumamoto, J, Goto, M, Denda, S, Nakatani, M, Takasugi, Y, Tsuchiya, K, Shimizu, Y, Takatsuru, Y & Denda, M 2013, 'External negative electric potential accelerates exocytosis of lamellar bodies in human skin ex vivo', Experimental Dermatology, vol. 22, no. 6, pp. 421-423. https://doi.org/10.1111/exd.12145
Kumamoto, Junichi ; Goto, Makiko ; Denda, Sumiko ; Nakatani, Masashi ; Takasugi, Yuya ; Tsuchiya, Katsunori ; Shimizu, Yuji ; Takatsuru, Yusuke ; Denda, Mitsuhiro. / External negative electric potential accelerates exocytosis of lamellar bodies in human skin ex vivo. In: Experimental Dermatology. 2013 ; Vol. 22, No. 6. pp. 421-423.
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AU - Tsuchiya, Katsunori

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