TY - JOUR
T1 - Extracellular Ca2+ uptake by T cells might help to make a diagnosis of acute rejection
AU - Akiyoshi, Kenjiro
AU - Hikida, Shigeki
AU - Inoue, Hiroyoshi
AU - Asagiri, Kimio
AU - Tanaka, Yoshiaki
AU - Yagi, Minoru
PY - 2007/2
Y1 - 2007/2
N2 - To more non-invasively diagnose acute rejection, we focused on the uptake of extracellular Ca2+ by T cells as a result of the activation of Ca2+ release activated Ca2+ channels. A full thickness of the skin allograft model was established using BN rats as the donors and LEW rats as the recipients, and similar LEW rats as both donors and recipients in the control group. After transplantation, the grafts were staged histopathologically in both rats. The uptake of extaracellular 45Ca pre T cell was measured in the macrophage-treated and non-treated groups, and the ratios between the two groups were calculated and the results were compared according to the post-operative day. No histopathological findings of acute rejection were observed in the control group. The allograft model group showed acute rejection histopathologically beginning on day 2 and increased through day 5. The macrophage-treated model/non-treated model 45Ca uptake ratio (CAR) was significantly higher in the allograft rats on day 2. No significant difference was observed on day 4. Measuring the uptake of extracellular Ca 2+ by recipient T cells using donor macrophages might be useful for making a diagnosis of acute rejection.
AB - To more non-invasively diagnose acute rejection, we focused on the uptake of extracellular Ca2+ by T cells as a result of the activation of Ca2+ release activated Ca2+ channels. A full thickness of the skin allograft model was established using BN rats as the donors and LEW rats as the recipients, and similar LEW rats as both donors and recipients in the control group. After transplantation, the grafts were staged histopathologically in both rats. The uptake of extaracellular 45Ca pre T cell was measured in the macrophage-treated and non-treated groups, and the ratios between the two groups were calculated and the results were compared according to the post-operative day. No histopathological findings of acute rejection were observed in the control group. The allograft model group showed acute rejection histopathologically beginning on day 2 and increased through day 5. The macrophage-treated model/non-treated model 45Ca uptake ratio (CAR) was significantly higher in the allograft rats on day 2. No significant difference was observed on day 4. Measuring the uptake of extracellular Ca 2+ by recipient T cells using donor macrophages might be useful for making a diagnosis of acute rejection.
KW - Acute rejection
KW - Ca release activated Ca (CRAC) channels
KW - T cell
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U2 - 10.1007/s00383-006-1842-1
DO - 10.1007/s00383-006-1842-1
M3 - Article
C2 - 17160685
AN - SCOPUS:33846610532
VL - 23
SP - 149
EP - 153
JO - Pediatric Surgery International
JF - Pediatric Surgery International
SN - 0179-0358
IS - 2
ER -