Extracellular Ca2+ uptake by T cells might help to make a diagnosis of acute rejection

Kenjiro Akiyoshi, Shigeki Hikida, Hiroyoshi Inoue, Kimio Asagiri, Yoshiaki Tanaka, Minoru Yagi

Research output: Contribution to journalArticle

Abstract

To more non-invasively diagnose acute rejection, we focused on the uptake of extracellular Ca2+ by T cells as a result of the activation of Ca2+ release activated Ca2+ channels. A full thickness of the skin allograft model was established using BN rats as the donors and LEW rats as the recipients, and similar LEW rats as both donors and recipients in the control group. After transplantation, the grafts were staged histopathologically in both rats. The uptake of extaracellular 45Ca pre T cell was measured in the macrophage-treated and non-treated groups, and the ratios between the two groups were calculated and the results were compared according to the post-operative day. No histopathological findings of acute rejection were observed in the control group. The allograft model group showed acute rejection histopathologically beginning on day 2 and increased through day 5. The macrophage-treated model/non-treated model 45Ca uptake ratio (CAR) was significantly higher in the allograft rats on day 2. No significant difference was observed on day 4. Measuring the uptake of extracellular Ca 2+ by recipient T cells using donor macrophages might be useful for making a diagnosis of acute rejection.

Original languageEnglish
Pages (from-to)149-153
Number of pages5
JournalPediatric surgery international
Volume23
Issue number2
DOIs
Publication statusPublished - 2007 Feb

Keywords

  • Acute rejection
  • Ca release activated Ca (CRAC) channels
  • T cell

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Surgery

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