Extracellular matrix metalloproteinase inducer in interstitial pneumonias

Nao Odajima, Tomoko Betsuyaku, Yasuyuki Nasuhara, Tomoo Itoh, Yuh Fukuda, Robert M. Senior, Masaharu Nishimura

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Extracellular matrix metalloproteinase inducer (EMMPRIN), a glycosylated transmembrane protein that induces matrix metalloproteinases (MMPs), is minimally expressed in the normal adult lung. We previously reported that it is up-regulated in murine bleomycin-induced lung injury. In this study, we determined the expression of EMMPRIN and its association with MMP-2, MMP-7, and MMP-9 in interstitial pneumonias (IPs). We performed immunohistochemistry for EMMPRIN and MMPs on lung tissue from 22 subjects with various IPs. We did bronchoalveolar lavage (BAL) on 9 of these subjects and 13 others with IPs to measure the soluble EMMPRIN in BAL fluid. For comparison, immunohistochemistry or BAL was done on 14 subjects without IPs. The staining intensity for each protein was scored from 0 to 3 in various epithelial cell types. Soluble EMMPRIN in BAL fluid was measured by an enzyme-linked immunosorbent assay. Extracellular matrix metalloproteinase inducer was prominent in abnormal epithelial cells. It was more prominent in hyperplastic type II cells, compared with epithelium in alveolar bronchiolization. It was also elevated in BAL fluid from the subjects with IPs. Matrix metalloproteinases were expressed in cells expressing EMMPRIN, although the profile of MMPs varied among the different abnormal epithelial cell types with MMP-2 and MMP-7 in hyperplastic type II cells and MMP-7 and MMP-9 in cells showing squamous metaplasia and cells comprising bronchiolization. These results suggest a role of EMMPRIN in reepithelialization in IPs.

Original languageEnglish
Pages (from-to)1058-1065
Number of pages8
JournalHuman Pathology
Volume37
Issue number8
DOIs
Publication statusPublished - 2006 Aug
Externally publishedYes

Fingerprint

CD147 Antigens
Interstitial Lung Diseases
Matrix Metalloproteinase 7
Matrix Metalloproteinases
Bronchoalveolar Lavage Fluid
Epithelial Cells
Matrix Metalloproteinase 2
Matrix Metalloproteinase 9
Bronchoalveolar Lavage
Immunohistochemistry
Lung
Metaplasia
Lung Injury
Proteins
Epithelium
Enzyme-Linked Immunosorbent Assay
Staining and Labeling

Keywords

  • Basigin
  • Bronchiolization
  • CD147
  • Matrix metalloproteinases
  • Squamous metaplasia

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Odajima, N., Betsuyaku, T., Nasuhara, Y., Itoh, T., Fukuda, Y., Senior, R. M., & Nishimura, M. (2006). Extracellular matrix metalloproteinase inducer in interstitial pneumonias. Human Pathology, 37(8), 1058-1065. https://doi.org/10.1016/j.humpath.2006.03.006

Extracellular matrix metalloproteinase inducer in interstitial pneumonias. / Odajima, Nao; Betsuyaku, Tomoko; Nasuhara, Yasuyuki; Itoh, Tomoo; Fukuda, Yuh; Senior, Robert M.; Nishimura, Masaharu.

In: Human Pathology, Vol. 37, No. 8, 08.2006, p. 1058-1065.

Research output: Contribution to journalArticle

Odajima, N, Betsuyaku, T, Nasuhara, Y, Itoh, T, Fukuda, Y, Senior, RM & Nishimura, M 2006, 'Extracellular matrix metalloproteinase inducer in interstitial pneumonias', Human Pathology, vol. 37, no. 8, pp. 1058-1065. https://doi.org/10.1016/j.humpath.2006.03.006
Odajima N, Betsuyaku T, Nasuhara Y, Itoh T, Fukuda Y, Senior RM et al. Extracellular matrix metalloproteinase inducer in interstitial pneumonias. Human Pathology. 2006 Aug;37(8):1058-1065. https://doi.org/10.1016/j.humpath.2006.03.006
Odajima, Nao ; Betsuyaku, Tomoko ; Nasuhara, Yasuyuki ; Itoh, Tomoo ; Fukuda, Yuh ; Senior, Robert M. ; Nishimura, Masaharu. / Extracellular matrix metalloproteinase inducer in interstitial pneumonias. In: Human Pathology. 2006 ; Vol. 37, No. 8. pp. 1058-1065.
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