Extracellular metabolite dynamics and temporal organization of metabolic function in E. coli

Martin Robert, Douglas Murray, Masayuki Honma, Kenji Nakahigashi, Tomoyoshi Soga, Masaru Tomita

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

Bacteria dynamically exchange with their environment by constantly uptaking nutrients and secreting metabolic products and other biomolecules. While such secreted metabolites may represent a high-level reporter of metabolic activity of the culture, relatively few studies have focused on their characterization. In addition, metabolites may be potential mediators of intercellular interactions. This study aims at identifying candidate mediators of intercellular exchanges and population behavior from temporal patterns of metabolites. To do this, we used capillary electrophoresis mass spectrometry (CE-MS) to monitor secreted metabolites in synchronized continuous culture of E. coli displaying respiratory oscillations. We observed that multiple metabolites are secreted in significant quantities in the extracellular medium, including amino acids and other intermediates of central metabolism. Some of the secreted metabolite dynamics appear linked to the known valine toxicity in E. coli and are also associated with the respiratory oscillations and their dynamics. Moreover, the dynamics in the level of several amino acids appeared well correlated, suggesting organized cycles of secretion/reuptake during respiratory and metabolic shifts linked to valine levels. Overall, the current results suggest that multiple metabolites are produced and likely exchanged by E. coli during continuous growth. These appear to reflect the internal metabolic state of the cell and may form an underappreciated level of information exchange that cell populations use to coordinate activities.

Original languageEnglish
Title of host publication2012 ICME International Conference on Complex Medical Engineering, CME 2012 Proceedings
Pages197-202
Number of pages6
DOIs
Publication statusPublished - 2012
Event6th International Conference on Complex Medical Engineering, CME 2012 - Kobe, Japan
Duration: 2012 Jul 12012 Jul 4

Other

Other6th International Conference on Complex Medical Engineering, CME 2012
CountryJapan
CityKobe
Period12/7/112/7/4

Fingerprint

Metabolites
Escherichia coli
Amino acids
Capillary electrophoresis
Biomolecules
Metabolism
Nutrients
Mass spectrometry
Toxicity
Bacteria
Cells

Keywords

  • cell-cell communication
  • dynamics
  • E. coli
  • metabolomics

ASJC Scopus subject areas

  • Biomedical Engineering

Cite this

Robert, M., Murray, D., Honma, M., Nakahigashi, K., Soga, T., & Tomita, M. (2012). Extracellular metabolite dynamics and temporal organization of metabolic function in E. coli. In 2012 ICME International Conference on Complex Medical Engineering, CME 2012 Proceedings (pp. 197-202). [6275650] https://doi.org/10.1109/ICCME.2012.6275650

Extracellular metabolite dynamics and temporal organization of metabolic function in E. coli. / Robert, Martin; Murray, Douglas; Honma, Masayuki; Nakahigashi, Kenji; Soga, Tomoyoshi; Tomita, Masaru.

2012 ICME International Conference on Complex Medical Engineering, CME 2012 Proceedings. 2012. p. 197-202 6275650.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Robert, M, Murray, D, Honma, M, Nakahigashi, K, Soga, T & Tomita, M 2012, Extracellular metabolite dynamics and temporal organization of metabolic function in E. coli. in 2012 ICME International Conference on Complex Medical Engineering, CME 2012 Proceedings., 6275650, pp. 197-202, 6th International Conference on Complex Medical Engineering, CME 2012, Kobe, Japan, 12/7/1. https://doi.org/10.1109/ICCME.2012.6275650
Robert M, Murray D, Honma M, Nakahigashi K, Soga T, Tomita M. Extracellular metabolite dynamics and temporal organization of metabolic function in E. coli. In 2012 ICME International Conference on Complex Medical Engineering, CME 2012 Proceedings. 2012. p. 197-202. 6275650 https://doi.org/10.1109/ICCME.2012.6275650
Robert, Martin ; Murray, Douglas ; Honma, Masayuki ; Nakahigashi, Kenji ; Soga, Tomoyoshi ; Tomita, Masaru. / Extracellular metabolite dynamics and temporal organization of metabolic function in E. coli. 2012 ICME International Conference on Complex Medical Engineering, CME 2012 Proceedings. 2012. pp. 197-202
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