Extracellular N-acetylaspartylglutamate released in the nucleus accumbens modulates the pain sensation

Analysis using a microdialysis/mass spectrometry integrated system

Moe Watanabe, Yuki Sugiura, Eiji Sugiyama, Michiko Narita, Edita Navratilova, Takashige Kondo, Naohiko Uchiyama, Akihiro Yamanaka, Naoko Kuzumaki, Frank Porreca, Minoru Narita

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Various small molecules act as neurotransmitters and orchestrate neural communication. Growing evidence suggests that not only classical neurotransmitters but also several small molecules, including amino acid derivatives, modulate synaptic transmission. As conditions of acute and chronic pain alter neuronal excitability in the nucleus accumbens, we hypothesized that small molecules released in the nucleus accumbens might play important roles in modulating the pain sensation. However, it is not easy to identify possible pain modulators owing to the absence of a method for comprehensively measuring extracellular small molecules in the brain. In this study, through the use of an emerging metabolomics technique, namely ion chromatography coupled with high-resolution mass spectrometry, we simultaneously analyzed the dynamics of more than 60 small molecules in brain fluids collected by microdialysis, under both the application of pain stimuli and the administration of analgesics. We identified N-acetylaspartylglutamate as a potential pain modulator that is endogenously released in the nucleus accumbens. Infusion of N-acetylaspartylglutamate into the nucleus accumbens significantly attenuated the pain induced by the activation of sensory nerves through optical stimulation. These findings suggest that N-acetylaspartylglutamate released in the nucleus accumbens could modulate pain sensation.

Original languageEnglish
JournalMolecular Pain
Volume14
DOIs
Publication statusPublished - 2018 Jan 1

Fingerprint

Microdialysis
Nucleus Accumbens
Mass Spectrometry
Pain
Neurotransmitter Agents
Metabolomics
Brain
Acute Pain
Synaptic Transmission
Chronic Pain
Analgesics
N-acetyl-1-aspartylglutamic acid
Chromatography
Communication
Ions
Amino Acids

Keywords

  • analgesia
  • dopamine
  • imaging mass spectrometry
  • in vivo microdialysis
  • mass spectrometry
  • morphine
  • N-acetylaspartylglutamate
  • nucleus accumbens
  • optogenetics
  • Pain

ASJC Scopus subject areas

  • Molecular Medicine
  • Cellular and Molecular Neuroscience
  • Anesthesiology and Pain Medicine

Cite this

Extracellular N-acetylaspartylglutamate released in the nucleus accumbens modulates the pain sensation : Analysis using a microdialysis/mass spectrometry integrated system. / Watanabe, Moe; Sugiura, Yuki; Sugiyama, Eiji; Narita, Michiko; Navratilova, Edita; Kondo, Takashige; Uchiyama, Naohiko; Yamanaka, Akihiro; Kuzumaki, Naoko; Porreca, Frank; Narita, Minoru.

In: Molecular Pain, Vol. 14, 01.01.2018.

Research output: Contribution to journalArticle

Watanabe, Moe ; Sugiura, Yuki ; Sugiyama, Eiji ; Narita, Michiko ; Navratilova, Edita ; Kondo, Takashige ; Uchiyama, Naohiko ; Yamanaka, Akihiro ; Kuzumaki, Naoko ; Porreca, Frank ; Narita, Minoru. / Extracellular N-acetylaspartylglutamate released in the nucleus accumbens modulates the pain sensation : Analysis using a microdialysis/mass spectrometry integrated system. In: Molecular Pain. 2018 ; Vol. 14.
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