Fabric reinforced polyacrylamide gels for electroblotting

Hideyuki Nishizawa, Ayako Murakami, Naoko Hayashi, Mami Iida, Yoshihiro Abe

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Abstract

The preparation of 0.5 mm fabric reinforced polyacrylamide (FRP) gels is described. After isoelectric focusing, egg white and isoelectric point marker proteins were quantitatively transferred in only 20 min from the FRP gels to nitrocellulose membranes by electroblotting. FRP gels combine the advantages of previously described supports for polyacrylamide gels with penetrability for electric current during blotting. The gels may be conveniently stored and afford operational versatility.

Original languageEnglish
Pages (from-to)349-350
Number of pages2
JournalElectrophoresis
Volume6
Issue number7
DOIs
Publication statusPublished - 1985

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ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

Cite this

Nishizawa, H., Murakami, A., Hayashi, N., Iida, M., & Abe, Y. (1985). Fabric reinforced polyacrylamide gels for electroblotting. Electrophoresis, 6(7), 349-350. https://doi.org/10.1002/elps.1150060708