Fabrication, characterization, and application of boron-doped diamond microelectrodes for in vivo dopamine detection

Akane Suzuki, Tribidasari A. Ivandini, Kenji Yoshimi, Akira Fujishima, Genko Oyama, Taizo Nakazato, Nobutaka Hattori, Shigeru Kitazawa, Yasuaki Einaga

Research output: Contribution to journalArticle

176 Citations (Scopus)

Abstract

Highly boron-doped diamond (BDD) was deposited on chemically etched micrometer-sized tungsten wires using microwave plasma assisted chemical vapor deposition (MPCVD), and these were used to fabricate BDD microelectrodes. BDD microelectrodes with very small diameter (about 5 μm) and 250 μm in length could be made successfully. In addition to the unique properties of BDD electrodes, such as a very low background current, high stability, and selective oxidation of dopamine (DA) in the presence of ascorbic acid (AA), other superior properties of the microelectrodes, including a constant current response, an increase in the mass transport, and the ability for use in high resistance media were also shown. An application study was conducted for in vivo detection of DA in mouse brain, where the BDD microelectrode was inserted into the corpus striatum of the mouse brain. A clear signal current response following medial forebrain bundle (MFB) stimulation could be obtained with high sensitivity. Excellent stability was achieved, indicating that the BDD microelectrodes are very promising for future in vivo electroanalysis.

Original languageEnglish
Pages (from-to)8608-8615
Number of pages8
JournalAnalytical chemistry
Volume79
Issue number22
DOIs
Publication statusPublished - 2007 Nov 15

ASJC Scopus subject areas

  • Analytical Chemistry

Fingerprint Dive into the research topics of 'Fabrication, characterization, and application of boron-doped diamond microelectrodes for in vivo dopamine detection'. Together they form a unique fingerprint.

  • Cite this

    Suzuki, A., Ivandini, T. A., Yoshimi, K., Fujishima, A., Oyama, G., Nakazato, T., Hattori, N., Kitazawa, S., & Einaga, Y. (2007). Fabrication, characterization, and application of boron-doped diamond microelectrodes for in vivo dopamine detection. Analytical chemistry, 79(22), 8608-8615. https://doi.org/10.1021/ac071519h