Abstract
We proposed a low cost fabrication procedure of a poly(methylmethacrylate) (PMMA) column chip. 3D microchannel structure consisting of four columns in a chip for a mother die was fabricated using dry film photoresist and photolithography technique. Electroforming was applied to the mother die in order to obtain a Ni mold, then, the pattern was transferred to PMMA by hot press. The column had a dam structure to keep enzyme-immobilized microbeads with volume of 640 nL. The column chip was applied for a micro flow injection analysis (μFIA) system. For a demonstration, we measured lactose using two columns in series. One column was set on upper stream and filled with chitosan microbeads immobilized with β-galactosidase, the other was on downstream and filled with the beads immobilized with glucose oxidase. The lactose detection was accomplished less than 90 s after the sample injection. The biosensing system also showed a high performance for lactose detection in wide range of 1 μM to 1 mM. These results show that the column chip and our microfluidic biosensing system have the potential to assist minuaturization with small sample volume and short determination time for a sequential analysis.
| Original language | English |
|---|---|
| Pages (from-to) | 707-712 |
| Number of pages | 6 |
| Journal | Talanta |
| Volume | 85 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2011 Jul 15 |
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Keywords
- μFIA
- Column chip
- Lactose
- PMMA
ASJC Scopus subject areas
- Chemistry(all)
Cite this
Fabrication of column chip made of PMMA for μfIA. / Ito, Takeshi; Kaneko, Satoru; Suzuki, Koji.
In: Talanta, Vol. 85, No. 1, 15.07.2011, p. 707-712.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Fabrication of column chip made of PMMA for μfIA
AU - Ito, Takeshi
AU - Kaneko, Satoru
AU - Suzuki, Koji
PY - 2011/7/15
Y1 - 2011/7/15
N2 - We proposed a low cost fabrication procedure of a poly(methylmethacrylate) (PMMA) column chip. 3D microchannel structure consisting of four columns in a chip for a mother die was fabricated using dry film photoresist and photolithography technique. Electroforming was applied to the mother die in order to obtain a Ni mold, then, the pattern was transferred to PMMA by hot press. The column had a dam structure to keep enzyme-immobilized microbeads with volume of 640 nL. The column chip was applied for a micro flow injection analysis (μFIA) system. For a demonstration, we measured lactose using two columns in series. One column was set on upper stream and filled with chitosan microbeads immobilized with β-galactosidase, the other was on downstream and filled with the beads immobilized with glucose oxidase. The lactose detection was accomplished less than 90 s after the sample injection. The biosensing system also showed a high performance for lactose detection in wide range of 1 μM to 1 mM. These results show that the column chip and our microfluidic biosensing system have the potential to assist minuaturization with small sample volume and short determination time for a sequential analysis.
AB - We proposed a low cost fabrication procedure of a poly(methylmethacrylate) (PMMA) column chip. 3D microchannel structure consisting of four columns in a chip for a mother die was fabricated using dry film photoresist and photolithography technique. Electroforming was applied to the mother die in order to obtain a Ni mold, then, the pattern was transferred to PMMA by hot press. The column had a dam structure to keep enzyme-immobilized microbeads with volume of 640 nL. The column chip was applied for a micro flow injection analysis (μFIA) system. For a demonstration, we measured lactose using two columns in series. One column was set on upper stream and filled with chitosan microbeads immobilized with β-galactosidase, the other was on downstream and filled with the beads immobilized with glucose oxidase. The lactose detection was accomplished less than 90 s after the sample injection. The biosensing system also showed a high performance for lactose detection in wide range of 1 μM to 1 mM. These results show that the column chip and our microfluidic biosensing system have the potential to assist minuaturization with small sample volume and short determination time for a sequential analysis.
KW - μFIA
KW - Column chip
KW - Lactose
KW - PMMA
UR - http://www.scopus.com/inward/record.url?scp=79958136821&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79958136821&partnerID=8YFLogxK
U2 - 10.1016/j.talanta.2011.04.053
DO - 10.1016/j.talanta.2011.04.053
M3 - Article
VL - 85
SP - 707
EP - 712
JO - Talanta
JF - Talanta
SN - 0039-9140
IS - 1
ER -