Fabrication of transplantable 3D-neuronal network

Midori Kato-Negishi, Yukiko Tsuda, Hiroaki Onoe, Shoji Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper describes a method to fabricate a transplantable three-dimensional (3D) neuronal network using the neu-rospheroid network (NSN) on a polydimethylsiloxane (PDMS) microchamber array. We developed a technique, termed the "NSN-stamping method", to transfer the NSN onto other substrates without any chemical treatment. Using this method, we demonstrate that different NSNs were stacked and formed 3D neuronal tissue, and then 3D-NSN could be transferred onto the cortical tissue at a high transfer rate. This method would be an extremely effective tool for tissue buildup in neuronal tissue engineering and provides a simple approach to the transplantation of 3D neuronal network.

Original languageEnglish
Title of host publication14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Pages632-634
Number of pages3
Publication statusPublished - 2010 Dec 1
Externally publishedYes
Event14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands
Duration: 2010 Oct 32010 Oct 7

Publication series

Name14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Volume1

Other

Other14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
CountryNetherlands
CityGroningen
Period10/10/310/10/7

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Keywords

  • Neuron
  • Neuronal network
  • Tissue engineering
  • Transplantation

ASJC Scopus subject areas

  • Control and Systems Engineering

Cite this

Kato-Negishi, M., Tsuda, Y., Onoe, H., & Takeuchi, S. (2010). Fabrication of transplantable 3D-neuronal network. In 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 (pp. 632-634). (14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010; Vol. 1).