Facile preparation of a fluorescence-labeled plasmid

Tsuyoshi Ishii, Yoshio Okahata, Toshinori Sato

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

We developed a facile method for labeling plasmid DNA with a fluorescent probe (FITC). The obtained FITC-labeled plasmid kept luciferase activity, and had enough fluorescence intensity to monitor with flow cytometer and confocal laser scanning microscope.

Original languageEnglish
Pages (from-to)386-387
Number of pages2
JournalChemistry Letters
Issue number4
Publication statusPublished - 2000
Externally publishedYes

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Fluorescein-5-isothiocyanate
Plasmids
Fluorescence
Luciferases
Fluorescent Dyes
Labeling
Microscopes
Scanning
Lasers
DNA

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Facile preparation of a fluorescence-labeled plasmid. / Ishii, Tsuyoshi; Okahata, Yoshio; Sato, Toshinori.

In: Chemistry Letters, No. 4, 2000, p. 386-387.

Research output: Contribution to journalArticle

Ishii, Tsuyoshi ; Okahata, Yoshio ; Sato, Toshinori. / Facile preparation of a fluorescence-labeled plasmid. In: Chemistry Letters. 2000 ; No. 4. pp. 386-387.
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