Factors contributing to salivary human immunodeficiency virus type-1 levels measured by a Poisson distribution-based PCR method

Ryo Ikeno, Eiko Yamada, Sayaka Yamazaki, Tomoyuki Ueda, Masaki Nagata, Ritsuo Takagi, Shingo Kato

Research output: Contribution to journalArticle

Abstract

Objective: To elucidate the mechanism underlying secretion of human immunodeficiency virus type 1 (HIV-1) into the oral cavity, by examining the relationships between various oral and systemic factors and the viral load in saliva. Methods: Plasma and saliva samples from HIV-1 infected patients were assayed using the COBAS® AmpliPrep/COBAS® TaqMan® HIV-1 Test, version 1.0 and a Poisson distribution-based polymerase chain reaction (PCR) method for quantifying HIV-1 RNA and DNA. Results: Forty-four pairs of samples were obtained from 18 patients. Salivary viral load was approximately 10% of the plasma viral load, but higher than the plasma load in two patients. The salivary viral DNA load was < 1% of the total HIV-1 nucleic acid load except in one patient who had more viral DNA than RNA. Multiple regression analysis showed that salivary viral load was significantly correlated with plasma viral load (partial correlation coefficient, 0.90) and the community periodontal index (–0.63). Conclusions: The present results suggest that excretion through salivary glands, but not occult bleeding, may be a major pathway of HIV-1 into the oral cavity.

Original languageEnglish
Pages (from-to)996-1007
Number of pages12
JournalJournal of International Medical Research
Volume46
Issue number3
DOIs
Publication statusPublished - 2018 Mar 1

Fingerprint

Poisson Distribution
Poisson distribution
Polymerase chain reaction
Viral Load
Viruses
HIV-1
Polymerase Chain Reaction
Plasmas
Viral DNA
Saliva
Mouth
RNA
Periodontal Index
Viral RNA
Salivary Glands
Regression analysis
Nucleic Acids
Regression Analysis
Hemorrhage
DNA

Keywords

  • HIV
  • plasma
  • Poisson distribution
  • quantification
  • saliva

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Biochemistry, medical

Cite this

Factors contributing to salivary human immunodeficiency virus type-1 levels measured by a Poisson distribution-based PCR method. / Ikeno, Ryo; Yamada, Eiko; Yamazaki, Sayaka; Ueda, Tomoyuki; Nagata, Masaki; Takagi, Ritsuo; Kato, Shingo.

In: Journal of International Medical Research, Vol. 46, No. 3, 01.03.2018, p. 996-1007.

Research output: Contribution to journalArticle

Ikeno, Ryo ; Yamada, Eiko ; Yamazaki, Sayaka ; Ueda, Tomoyuki ; Nagata, Masaki ; Takagi, Ritsuo ; Kato, Shingo. / Factors contributing to salivary human immunodeficiency virus type-1 levels measured by a Poisson distribution-based PCR method. In: Journal of International Medical Research. 2018 ; Vol. 46, No. 3. pp. 996-1007.
@article{cc486b9e11854db7bac5a7cea500ec92,
title = "Factors contributing to salivary human immunodeficiency virus type-1 levels measured by a Poisson distribution-based PCR method",
abstract = "Objective: To elucidate the mechanism underlying secretion of human immunodeficiency virus type 1 (HIV-1) into the oral cavity, by examining the relationships between various oral and systemic factors and the viral load in saliva. Methods: Plasma and saliva samples from HIV-1 infected patients were assayed using the COBAS{\circledR} AmpliPrep/COBAS{\circledR} TaqMan{\circledR} HIV-1 Test, version 1.0 and a Poisson distribution-based polymerase chain reaction (PCR) method for quantifying HIV-1 RNA and DNA. Results: Forty-four pairs of samples were obtained from 18 patients. Salivary viral load was approximately 10{\%} of the plasma viral load, but higher than the plasma load in two patients. The salivary viral DNA load was < 1{\%} of the total HIV-1 nucleic acid load except in one patient who had more viral DNA than RNA. Multiple regression analysis showed that salivary viral load was significantly correlated with plasma viral load (partial correlation coefficient, 0.90) and the community periodontal index (–0.63). Conclusions: The present results suggest that excretion through salivary glands, but not occult bleeding, may be a major pathway of HIV-1 into the oral cavity.",
keywords = "HIV, plasma, Poisson distribution, quantification, saliva",
author = "Ryo Ikeno and Eiko Yamada and Sayaka Yamazaki and Tomoyuki Ueda and Masaki Nagata and Ritsuo Takagi and Shingo Kato",
year = "2018",
month = "3",
day = "1",
doi = "10.1177/0300060517728652",
language = "English",
volume = "46",
pages = "996--1007",
journal = "Journal of International Medical Research",
issn = "0300-0605",
publisher = "Field House Publishing LLP",
number = "3",

}

TY - JOUR

T1 - Factors contributing to salivary human immunodeficiency virus type-1 levels measured by a Poisson distribution-based PCR method

AU - Ikeno, Ryo

AU - Yamada, Eiko

AU - Yamazaki, Sayaka

AU - Ueda, Tomoyuki

AU - Nagata, Masaki

AU - Takagi, Ritsuo

AU - Kato, Shingo

PY - 2018/3/1

Y1 - 2018/3/1

N2 - Objective: To elucidate the mechanism underlying secretion of human immunodeficiency virus type 1 (HIV-1) into the oral cavity, by examining the relationships between various oral and systemic factors and the viral load in saliva. Methods: Plasma and saliva samples from HIV-1 infected patients were assayed using the COBAS® AmpliPrep/COBAS® TaqMan® HIV-1 Test, version 1.0 and a Poisson distribution-based polymerase chain reaction (PCR) method for quantifying HIV-1 RNA and DNA. Results: Forty-four pairs of samples were obtained from 18 patients. Salivary viral load was approximately 10% of the plasma viral load, but higher than the plasma load in two patients. The salivary viral DNA load was < 1% of the total HIV-1 nucleic acid load except in one patient who had more viral DNA than RNA. Multiple regression analysis showed that salivary viral load was significantly correlated with plasma viral load (partial correlation coefficient, 0.90) and the community periodontal index (–0.63). Conclusions: The present results suggest that excretion through salivary glands, but not occult bleeding, may be a major pathway of HIV-1 into the oral cavity.

AB - Objective: To elucidate the mechanism underlying secretion of human immunodeficiency virus type 1 (HIV-1) into the oral cavity, by examining the relationships between various oral and systemic factors and the viral load in saliva. Methods: Plasma and saliva samples from HIV-1 infected patients were assayed using the COBAS® AmpliPrep/COBAS® TaqMan® HIV-1 Test, version 1.0 and a Poisson distribution-based polymerase chain reaction (PCR) method for quantifying HIV-1 RNA and DNA. Results: Forty-four pairs of samples were obtained from 18 patients. Salivary viral load was approximately 10% of the plasma viral load, but higher than the plasma load in two patients. The salivary viral DNA load was < 1% of the total HIV-1 nucleic acid load except in one patient who had more viral DNA than RNA. Multiple regression analysis showed that salivary viral load was significantly correlated with plasma viral load (partial correlation coefficient, 0.90) and the community periodontal index (–0.63). Conclusions: The present results suggest that excretion through salivary glands, but not occult bleeding, may be a major pathway of HIV-1 into the oral cavity.

KW - HIV

KW - plasma

KW - Poisson distribution

KW - quantification

KW - saliva

UR - http://www.scopus.com/inward/record.url?scp=85043378423&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85043378423&partnerID=8YFLogxK

U2 - 10.1177/0300060517728652

DO - 10.1177/0300060517728652

M3 - Article

VL - 46

SP - 996

EP - 1007

JO - Journal of International Medical Research

JF - Journal of International Medical Research

SN - 0300-0605

IS - 3

ER -