Fas-mediated apoptosis in Jurkat cells is suppressed in the pre-G2/M phase

N. Hiroi, H. Maruta, S. Tanuma

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The relationship between the cell cycle and Fas-mediated apoptosis was investigated using Jurkat cells. Analysis of the inducibility of apoptosis by anti-Fas antibody during the cell cycle synchronized by the thymidine double- block method, showed that apoptosis was induced in only 50% of the G2/M phase cells, while most of cells in the other phases underwent apoptosis. These observations indicate that G2/M phase cells are more resistant to Fas- mediated apoptosis than cells in other phases. Furthermore, a detailed analysis of G2/M phase found that only 20-30% of the cells underwent apoptosis 12 h after the removal of the second thymidine block (pre-G2/M phase). This suggests that Fas-mediated apoptosis is potently suppressed during the pre-G2/M phase. A possible explanation for the observation that cells in the pre-G2/M phase are less sensitive to anti-Fas antibody is lower expression level of Fas. To test this possibility, Fas expression levels on the cell surface during the cell cycle were examined. The content of Fas on the cell surface, however, did not change appreciably during the cell cycle. Thus, the suppression of apoptosis in the pre-G2/M phase is determined downstream after the receipt of the apoptotic signal through Fas.

Original languageEnglish
Pages (from-to)255-261
Number of pages7
JournalApoptosis
Volume4
Issue number4
DOIs
Publication statusPublished - 1999
Externally publishedYes

Fingerprint

Jurkat Cells
G2 Phase
Cell Division
Apoptosis
Cell Cycle
Cells
Thymidine
Anti-Idiotypic Antibodies
Antibodies

Keywords

  • Apoptosis
  • Cell cycle
  • Fas
  • G2/M phase
  • Jurkat cell

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Cell Biology

Cite this

Fas-mediated apoptosis in Jurkat cells is suppressed in the pre-G2/M phase. / Hiroi, N.; Maruta, H.; Tanuma, S.

In: Apoptosis, Vol. 4, No. 4, 1999, p. 255-261.

Research output: Contribution to journalArticle

Hiroi, N. ; Maruta, H. ; Tanuma, S. / Fas-mediated apoptosis in Jurkat cells is suppressed in the pre-G2/M phase. In: Apoptosis. 1999 ; Vol. 4, No. 4. pp. 255-261.
@article{3a4881352010487d881e3202a8637a77,
title = "Fas-mediated apoptosis in Jurkat cells is suppressed in the pre-G2/M phase",
abstract = "The relationship between the cell cycle and Fas-mediated apoptosis was investigated using Jurkat cells. Analysis of the inducibility of apoptosis by anti-Fas antibody during the cell cycle synchronized by the thymidine double- block method, showed that apoptosis was induced in only 50{\%} of the G2/M phase cells, while most of cells in the other phases underwent apoptosis. These observations indicate that G2/M phase cells are more resistant to Fas- mediated apoptosis than cells in other phases. Furthermore, a detailed analysis of G2/M phase found that only 20-30{\%} of the cells underwent apoptosis 12 h after the removal of the second thymidine block (pre-G2/M phase). This suggests that Fas-mediated apoptosis is potently suppressed during the pre-G2/M phase. A possible explanation for the observation that cells in the pre-G2/M phase are less sensitive to anti-Fas antibody is lower expression level of Fas. To test this possibility, Fas expression levels on the cell surface during the cell cycle were examined. The content of Fas on the cell surface, however, did not change appreciably during the cell cycle. Thus, the suppression of apoptosis in the pre-G2/M phase is determined downstream after the receipt of the apoptotic signal through Fas.",
keywords = "Apoptosis, Cell cycle, Fas, G2/M phase, Jurkat cell",
author = "N. Hiroi and H. Maruta and S. Tanuma",
year = "1999",
doi = "10.1023/A:1009652825846",
language = "English",
volume = "4",
pages = "255--261",
journal = "Apoptosis : an international journal on programmed cell death",
issn = "1360-8185",
publisher = "Springer Netherlands",
number = "4",

}

TY - JOUR

T1 - Fas-mediated apoptosis in Jurkat cells is suppressed in the pre-G2/M phase

AU - Hiroi, N.

AU - Maruta, H.

AU - Tanuma, S.

PY - 1999

Y1 - 1999

N2 - The relationship between the cell cycle and Fas-mediated apoptosis was investigated using Jurkat cells. Analysis of the inducibility of apoptosis by anti-Fas antibody during the cell cycle synchronized by the thymidine double- block method, showed that apoptosis was induced in only 50% of the G2/M phase cells, while most of cells in the other phases underwent apoptosis. These observations indicate that G2/M phase cells are more resistant to Fas- mediated apoptosis than cells in other phases. Furthermore, a detailed analysis of G2/M phase found that only 20-30% of the cells underwent apoptosis 12 h after the removal of the second thymidine block (pre-G2/M phase). This suggests that Fas-mediated apoptosis is potently suppressed during the pre-G2/M phase. A possible explanation for the observation that cells in the pre-G2/M phase are less sensitive to anti-Fas antibody is lower expression level of Fas. To test this possibility, Fas expression levels on the cell surface during the cell cycle were examined. The content of Fas on the cell surface, however, did not change appreciably during the cell cycle. Thus, the suppression of apoptosis in the pre-G2/M phase is determined downstream after the receipt of the apoptotic signal through Fas.

AB - The relationship between the cell cycle and Fas-mediated apoptosis was investigated using Jurkat cells. Analysis of the inducibility of apoptosis by anti-Fas antibody during the cell cycle synchronized by the thymidine double- block method, showed that apoptosis was induced in only 50% of the G2/M phase cells, while most of cells in the other phases underwent apoptosis. These observations indicate that G2/M phase cells are more resistant to Fas- mediated apoptosis than cells in other phases. Furthermore, a detailed analysis of G2/M phase found that only 20-30% of the cells underwent apoptosis 12 h after the removal of the second thymidine block (pre-G2/M phase). This suggests that Fas-mediated apoptosis is potently suppressed during the pre-G2/M phase. A possible explanation for the observation that cells in the pre-G2/M phase are less sensitive to anti-Fas antibody is lower expression level of Fas. To test this possibility, Fas expression levels on the cell surface during the cell cycle were examined. The content of Fas on the cell surface, however, did not change appreciably during the cell cycle. Thus, the suppression of apoptosis in the pre-G2/M phase is determined downstream after the receipt of the apoptotic signal through Fas.

KW - Apoptosis

KW - Cell cycle

KW - Fas

KW - G2/M phase

KW - Jurkat cell

UR - http://www.scopus.com/inward/record.url?scp=0032885074&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032885074&partnerID=8YFLogxK

U2 - 10.1023/A:1009652825846

DO - 10.1023/A:1009652825846

M3 - Article

C2 - 14634276

AN - SCOPUS:0032885074

VL - 4

SP - 255

EP - 261

JO - Apoptosis : an international journal on programmed cell death

JF - Apoptosis : an international journal on programmed cell death

SN - 1360-8185

IS - 4

ER -