Fate of unstable Bacillus subtilis subgenome: Re-integration and amplification in the main genome

Mitsuhiro Itaya, Teruo Tanaka

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The plastic Bacillus subtilis genome was dissected into two physically separate genomes, the 3.9 Mb main genome and the 0.3 Mb subgenome. DNA replication of the main genome was initiated from the normal replication origin (oriC) and that of the subgenome was from a 7.2 kb oriN-containing fragment artificially inserted. When the 7.2 kb fragment was shortened to a 1.5 kb fragment that contains oriN but lacks the segregational function, the subgenome became unstable and was rapidly lost from the cell, producing inviable cells due to the loss of essential genes carried by the subgenome. Stable survivors were isolated in which the subgenome had re-integrated and multiplied in the main genome. These results suggest that a reduced genetic stability of the subgenome induces size variation of the B. subtilis genome. Copyright (C) 1999 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)235-238
Number of pages4
JournalFEBS Letters
Volume448
Issue number2-3
DOIs
Publication statusPublished - 1999 Apr 9

Keywords

  • Essential gene
  • Homologous recombination
  • Subgenome
  • oriN
  • repN

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Fingerprint Dive into the research topics of 'Fate of unstable Bacillus subtilis subgenome: Re-integration and amplification in the main genome'. Together they form a unique fingerprint.

  • Cite this