Feasibility of noninvasive evaluation of biophysical properties of tissue-engineered cartilage by using quantitative MRI

Shogo Miyata, Tomokazu Numano, Kazuhiro Homma, Tetsuya Tateishi, Takashi Ushida

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The application of tissue-engineered cartilage in a clinical setting requires a noninvasive method to assess the biophysical and biochemical properties of the engineered cartilage. Since articular cartilage is composed of 70-80% water and has dense extracellular matrixes (ECM), it is considered that the condition of the water molecules in the tissue is correlated with its biomechanical property. Therefore, magnetic resonance imaging (MRI) represents a potential approach to assess the biophysical property of the engineered cartilage. In this study, we test the hypothesis that quantitative MRI can be used as a noninvasive assessment method to assess the biophysical property of the engineered cartilage. To reconstruct a model of cartilaginous tissue, chondrocytes harvested from the humeral head of calves were embedded in an agarose gel and cultured in vitro up to 4 weeks. Equilibrium Young's moduli were determined from the stress relaxation tests. After mechanical testing, MRI-derived parameters (longitudinal relaxation time T1, transverse relaxation time T2, and water self-diffusion coefficient D) were measured. The equilibrium Young's modulus of the engineered cartilage showed a tendency to increase with an increase in the culture time, whereas T1 and D decreased. Based on a regression analysis, T1 and D showed a strong correlation with the equilibrium Young's modulus. The results showed that T1 and D values derived from the MRI measurements could be used to noninvasively monitor the biophysical properties of the engineered cartilage.

Original languageEnglish
Pages (from-to)2990-2998
Number of pages9
JournalJournal of Biomechanics
Volume40
Issue number13
DOIs
Publication statusPublished - 2007
Externally publishedYes

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Cartilage
Magnetic resonance
Magnetic Resonance Imaging
Tissue
Imaging techniques
Elastic Modulus
Elastic moduli
Water
Relaxation time
Humeral Head
Articular Cartilage
Chondrocytes
Exercise Test
Mechanical testing
Sepharose
Stress relaxation
Extracellular Matrix
Regression analysis
Gels
Regression Analysis

Keywords

  • Chondrocytes
  • MRI
  • Noninvasive assessment
  • Tissue engineering

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Cite this

Feasibility of noninvasive evaluation of biophysical properties of tissue-engineered cartilage by using quantitative MRI. / Miyata, Shogo; Numano, Tomokazu; Homma, Kazuhiro; Tateishi, Tetsuya; Ushida, Takashi.

In: Journal of Biomechanics, Vol. 40, No. 13, 2007, p. 2990-2998.

Research output: Contribution to journalArticle

Miyata, Shogo ; Numano, Tomokazu ; Homma, Kazuhiro ; Tateishi, Tetsuya ; Ushida, Takashi. / Feasibility of noninvasive evaluation of biophysical properties of tissue-engineered cartilage by using quantitative MRI. In: Journal of Biomechanics. 2007 ; Vol. 40, No. 13. pp. 2990-2998.
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