Feeder-free 3D culture system to expand mouse induced pluripotent stem cells in hydrogel core-shell microfiber

K. Ikeda, T. Okitsu, Hiroaki Onoe, A. Y. Hsiao, S. Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This conference proceeding describes the culturing and expansion of mouse induced pluripotent stem cells (iPSCs) in hydrogel core-shell microfiber; the core consists of iPSCs and extracellular matrix (ECM) proteins, and the shell is composed of calcium alginate. This culture system is established by using a microfluidic co-axial device. The mouse iPSCs in this feeder-free 3D culture system expand with sustained pluripotency for nine days, even though their expansion ratio decrease to almost half of that when cultured by conventional 2D system on feeder cells. We believe that this feeder-free 3D culture system would be useful to obtain high-quality iPSCs to apply them in various fields including pharmaceutical and medicine, where contamination of feeder cells need to be avoided.

Original languageEnglish
Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PublisherChemical and Biological Microsystems Society
Pages910-911
Number of pages2
ISBN (Print)9780979806476
Publication statusPublished - 2014
Externally publishedYes
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
Duration: 2014 Oct 262014 Oct 30

Other

Other18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
CountryUnited States
CitySan Antonio
Period14/10/2614/10/30

Fingerprint

Stem cells
Cell culture
Hydrogels
Alginate
Microfluidics
Drug products
Medicine
Calcium
Contamination
Proteins

Keywords

  • 3D culture system
  • Core-shell microfiber
  • Induced pluripotent stem cell
  • Pluripotency

ASJC Scopus subject areas

  • Control and Systems Engineering

Cite this

Ikeda, K., Okitsu, T., Onoe, H., Hsiao, A. Y., & Takeuchi, S. (2014). Feeder-free 3D culture system to expand mouse induced pluripotent stem cells in hydrogel core-shell microfiber. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 (pp. 910-911). Chemical and Biological Microsystems Society.

Feeder-free 3D culture system to expand mouse induced pluripotent stem cells in hydrogel core-shell microfiber. / Ikeda, K.; Okitsu, T.; Onoe, Hiroaki; Hsiao, A. Y.; Takeuchi, S.

18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. p. 910-911.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Ikeda, K, Okitsu, T, Onoe, H, Hsiao, AY & Takeuchi, S 2014, Feeder-free 3D culture system to expand mouse induced pluripotent stem cells in hydrogel core-shell microfiber. in 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, pp. 910-911, 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, San Antonio, United States, 14/10/26.
Ikeda K, Okitsu T, Onoe H, Hsiao AY, Takeuchi S. Feeder-free 3D culture system to expand mouse induced pluripotent stem cells in hydrogel core-shell microfiber. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society. 2014. p. 910-911
Ikeda, K. ; Okitsu, T. ; Onoe, Hiroaki ; Hsiao, A. Y. ; Takeuchi, S. / Feeder-free 3D culture system to expand mouse induced pluripotent stem cells in hydrogel core-shell microfiber. 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. pp. 910-911
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