Femto-liter protein purification by parallel two-phase nanofluidics

Shu Matsuura, Yutaka Kazoe, Takehiko Kitamori

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We succeeded in the formation of aqueous parallel two-phase flow in nanochannel for the first time and applied it to fL protein purification. As a result, the removal of 79% lipid and the remains of 91% protein were realized at two-phase contact time of 10 ms and the volume of 16 fL which was much smaller than the volume of single cell (pL). Therefore, this method will greatly contribute to development of pretreatment process in single cell protein analysis.

Original languageEnglish
Title of host publication23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
PublisherChemical and Biological Microsystems Society
Pages618-619
Number of pages2
ISBN (Electronic)9781733419000
Publication statusPublished - 2019
Externally publishedYes
Event23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 - Basel, Switzerland
Duration: 2019 Oct 272019 Oct 31

Publication series

Name23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

Conference

Conference23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
CountrySwitzerland
CityBasel
Period19/10/2719/10/31

Keywords

  • Aqueous two-phase system
  • Nanochannel
  • Nanofluidics
  • Purification

ASJC Scopus subject areas

  • Bioengineering
  • Chemical Engineering (miscellaneous)

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