Femtoliter nanofluidic valve utilizing glass deformation

Yutaka Kazoe, Yuriy Pihosh, Hitomi Takahashi, Takeshi Ohyama, Hiroki Sano, Kyojiro Morikawa, Kazuma Mawatari, Takehiko Kitamori

Research output: Contribution to journalArticle

Abstract

In the field of micro/nanofluidics, the channel open/close valves are among the most important technologies for switching and partitioning actions and integration of various operations into fluidic circuits. While several types of valves have been developed in microfluidics, few are capable in nanofluidics. In this study, we proposed a femtoliter (fL) volume nanochannel open/close valve fabricated in glass substrates. The valve consists of a shallow, circular and stepped-bottom valve chamber connected to nanochannels and an actuator. Even with tiny deformation occurring at the nanolevel in glass, an open/closed state of a nanochannel (10-1000 nm) can be achieved. We designed a fL-valve based on an analytical material deformation model, and developed a valve fabrication process. We then verified the open/closed state of the valve using a 308 fL-valve chamber with a four-stepped nanostructure fitting an arc-shape of deflected glass, confirmed its stability and durability over 50 open/close operations, and succeeded in stopping/flowing an aqueous solution at 209 fL s -1 under a 100 kPa pressure in a 900 nm nanochannel with a fast response of ∼0.65 s. A leak flow from the closed valve was sufficiently small even at a 490 kPa pressure-driven flow. Since the developed fL-valve can be applied to various nanofluidic devices made of glass and other rigid materials such as plastic, it is expected that this work will contribute significantly to the development of novel integrated micro/nanofluidics chemical systems for use in various applications, such as single cell/single molecule analysis.

Original languageEnglish
Pages (from-to)1686-1694
Number of pages9
JournalLab on a Chip
Volume19
Issue number9
DOIs
Publication statusPublished - 2019 Jan 1

Fingerprint

Nanofluidics
Glass
Single-Cell Analysis
Pressure
Microfluidics
Nanostructures
Fluidics
Plastics
Durability
Actuators
Technology
Fabrication
Equipment and Supplies
Molecules
Networks (circuits)
Substrates

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

Cite this

Kazoe, Y., Pihosh, Y., Takahashi, H., Ohyama, T., Sano, H., Morikawa, K., ... Kitamori, T. (2019). Femtoliter nanofluidic valve utilizing glass deformation. Lab on a Chip, 19(9), 1686-1694. https://doi.org/10.1039/c8lc01340c

Femtoliter nanofluidic valve utilizing glass deformation. / Kazoe, Yutaka; Pihosh, Yuriy; Takahashi, Hitomi; Ohyama, Takeshi; Sano, Hiroki; Morikawa, Kyojiro; Mawatari, Kazuma; Kitamori, Takehiko.

In: Lab on a Chip, Vol. 19, No. 9, 01.01.2019, p. 1686-1694.

Research output: Contribution to journalArticle

Kazoe, Y, Pihosh, Y, Takahashi, H, Ohyama, T, Sano, H, Morikawa, K, Mawatari, K & Kitamori, T 2019, 'Femtoliter nanofluidic valve utilizing glass deformation', Lab on a Chip, vol. 19, no. 9, pp. 1686-1694. https://doi.org/10.1039/c8lc01340c
Kazoe Y, Pihosh Y, Takahashi H, Ohyama T, Sano H, Morikawa K et al. Femtoliter nanofluidic valve utilizing glass deformation. Lab on a Chip. 2019 Jan 1;19(9):1686-1694. https://doi.org/10.1039/c8lc01340c
Kazoe, Yutaka ; Pihosh, Yuriy ; Takahashi, Hitomi ; Ohyama, Takeshi ; Sano, Hiroki ; Morikawa, Kyojiro ; Mawatari, Kazuma ; Kitamori, Takehiko. / Femtoliter nanofluidic valve utilizing glass deformation. In: Lab on a Chip. 2019 ; Vol. 19, No. 9. pp. 1686-1694.
@article{83033613ef294f57b81348cc31eaeb92,
title = "Femtoliter nanofluidic valve utilizing glass deformation",
abstract = "In the field of micro/nanofluidics, the channel open/close valves are among the most important technologies for switching and partitioning actions and integration of various operations into fluidic circuits. While several types of valves have been developed in microfluidics, few are capable in nanofluidics. In this study, we proposed a femtoliter (fL) volume nanochannel open/close valve fabricated in glass substrates. The valve consists of a shallow, circular and stepped-bottom valve chamber connected to nanochannels and an actuator. Even with tiny deformation occurring at the nanolevel in glass, an open/closed state of a nanochannel (10-1000 nm) can be achieved. We designed a fL-valve based on an analytical material deformation model, and developed a valve fabrication process. We then verified the open/closed state of the valve using a 308 fL-valve chamber with a four-stepped nanostructure fitting an arc-shape of deflected glass, confirmed its stability and durability over 50 open/close operations, and succeeded in stopping/flowing an aqueous solution at 209 fL s -1 under a 100 kPa pressure in a 900 nm nanochannel with a fast response of ∼0.65 s. A leak flow from the closed valve was sufficiently small even at a 490 kPa pressure-driven flow. Since the developed fL-valve can be applied to various nanofluidic devices made of glass and other rigid materials such as plastic, it is expected that this work will contribute significantly to the development of novel integrated micro/nanofluidics chemical systems for use in various applications, such as single cell/single molecule analysis.",
author = "Yutaka Kazoe and Yuriy Pihosh and Hitomi Takahashi and Takeshi Ohyama and Hiroki Sano and Kyojiro Morikawa and Kazuma Mawatari and Takehiko Kitamori",
year = "2019",
month = "1",
day = "1",
doi = "10.1039/c8lc01340c",
language = "English",
volume = "19",
pages = "1686--1694",
journal = "Lab on a Chip - Miniaturisation for Chemistry and Biology",
issn = "1473-0197",
publisher = "Royal Society of Chemistry",
number = "9",

}

TY - JOUR

T1 - Femtoliter nanofluidic valve utilizing glass deformation

AU - Kazoe, Yutaka

AU - Pihosh, Yuriy

AU - Takahashi, Hitomi

AU - Ohyama, Takeshi

AU - Sano, Hiroki

AU - Morikawa, Kyojiro

AU - Mawatari, Kazuma

AU - Kitamori, Takehiko

PY - 2019/1/1

Y1 - 2019/1/1

N2 - In the field of micro/nanofluidics, the channel open/close valves are among the most important technologies for switching and partitioning actions and integration of various operations into fluidic circuits. While several types of valves have been developed in microfluidics, few are capable in nanofluidics. In this study, we proposed a femtoliter (fL) volume nanochannel open/close valve fabricated in glass substrates. The valve consists of a shallow, circular and stepped-bottom valve chamber connected to nanochannels and an actuator. Even with tiny deformation occurring at the nanolevel in glass, an open/closed state of a nanochannel (10-1000 nm) can be achieved. We designed a fL-valve based on an analytical material deformation model, and developed a valve fabrication process. We then verified the open/closed state of the valve using a 308 fL-valve chamber with a four-stepped nanostructure fitting an arc-shape of deflected glass, confirmed its stability and durability over 50 open/close operations, and succeeded in stopping/flowing an aqueous solution at 209 fL s -1 under a 100 kPa pressure in a 900 nm nanochannel with a fast response of ∼0.65 s. A leak flow from the closed valve was sufficiently small even at a 490 kPa pressure-driven flow. Since the developed fL-valve can be applied to various nanofluidic devices made of glass and other rigid materials such as plastic, it is expected that this work will contribute significantly to the development of novel integrated micro/nanofluidics chemical systems for use in various applications, such as single cell/single molecule analysis.

AB - In the field of micro/nanofluidics, the channel open/close valves are among the most important technologies for switching and partitioning actions and integration of various operations into fluidic circuits. While several types of valves have been developed in microfluidics, few are capable in nanofluidics. In this study, we proposed a femtoliter (fL) volume nanochannel open/close valve fabricated in glass substrates. The valve consists of a shallow, circular and stepped-bottom valve chamber connected to nanochannels and an actuator. Even with tiny deformation occurring at the nanolevel in glass, an open/closed state of a nanochannel (10-1000 nm) can be achieved. We designed a fL-valve based on an analytical material deformation model, and developed a valve fabrication process. We then verified the open/closed state of the valve using a 308 fL-valve chamber with a four-stepped nanostructure fitting an arc-shape of deflected glass, confirmed its stability and durability over 50 open/close operations, and succeeded in stopping/flowing an aqueous solution at 209 fL s -1 under a 100 kPa pressure in a 900 nm nanochannel with a fast response of ∼0.65 s. A leak flow from the closed valve was sufficiently small even at a 490 kPa pressure-driven flow. Since the developed fL-valve can be applied to various nanofluidic devices made of glass and other rigid materials such as plastic, it is expected that this work will contribute significantly to the development of novel integrated micro/nanofluidics chemical systems for use in various applications, such as single cell/single molecule analysis.

UR - http://www.scopus.com/inward/record.url?scp=85064975407&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85064975407&partnerID=8YFLogxK

U2 - 10.1039/c8lc01340c

DO - 10.1039/c8lc01340c

M3 - Article

VL - 19

SP - 1686

EP - 1694

JO - Lab on a Chip - Miniaturisation for Chemistry and Biology

JF - Lab on a Chip - Miniaturisation for Chemistry and Biology

SN - 1473-0197

IS - 9

ER -