Fiber optic light-scattering measurement system for evaluation of embryo viability: light-scattering characteristics from live mouse embryo

Harumi Itoh, Tsunenori M.D. Arai, Makoto Kikuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

We measured angular distribution of the light scattering from live mouse embryo with 632.8nm in wavelength to evaluate the embryo viability. We aim to measure the mitochondrial density in human embryo which have relation to the embryo viability. We have constructed the light scattering measurement system to detect the mitochondrial density non-invasively. We have employed two optical fibers for the illumination and sensing to change the angle between these fibers. There were two dips on the scattering angular distribution from the embryo. These dips existed on 30 and 85 deg. We calculated the scattering angular pattern by Mie theory to fit the measured scattering estimated scattering size and density. The best fitting was obtained when the particle size and density were 0.9 μm and 1010 particles per ml, respectively. These values coincided with the approximated values of mitochondrial in the embryo. The measured light scattering may mainly originated from mitochondria in spite of the existence of the various scattering particles in the embryo. Since our simple scattering measurement may offer the mitochondrial density in the embryo, it might become the practical method of human embryo on in vitro fertilization-embryo transfer.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
PublisherSociety of Photo-Optical Instrumentation Engineers
Pages108-114
Number of pages7
ISBN (Print)0819423866
Publication statusPublished - 1997 Dec 1
EventLaser-Tissue Interaction VIII - San Jose, CA, USA
Duration: 1997 Feb 91997 Feb 12

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume2975
ISSN (Print)0277-786X

Other

OtherLaser-Tissue Interaction VIII
CitySan Jose, CA, USA
Period97/2/997/2/12

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ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

Cite this

Itoh, H., Arai, T. M. D., & Kikuchi, M. (1997). Fiber optic light-scattering measurement system for evaluation of embryo viability: light-scattering characteristics from live mouse embryo. In Proceedings of SPIE - The International Society for Optical Engineering (pp. 108-114). (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 2975). Society of Photo-Optical Instrumentation Engineers.