Fiber optic light-scattering measurement system for evaluation of embryo viability: model experiment

Harumi Itoh, Tsunenori M.D. Arai, Makoto Kikuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We evaluated the particle density detectability and particle size detectivity of our fiber-optic light-scattering measurement system. In order to prevent the multiple pregnancy on current in vitro fertilization-embryo transfer, we have aimed to develop a new quantitative and non- invasive method to select a single viable human embryo. We employed the measurement of mitochondria localization in an embryo, which may have the correlation with development ability. We applied the angular distribution measurement of the light-scattering intensity from the embryo to obtain the information originated from the mitochondria. The latex spheres with a diameter of 1.0 μm were used to simulate the scattering intensity of the mitochondria. The measurement probes of our system consisted of two fibers for illumination and sensing. They were arranged at a right angle to a microscope optical axis to measure the angular distribution of the light-scattering intensity. We observed that the light-scattering intensity increased monotonically in the range from 10 6 to 10 10 particles per ml. Since the mitochondria density in a human embryo corresponded to 2.5 × 10 7 per ml in the measurement chamber, we may measure the mitochondria density in the human embryo. The angular dependence of light-scattering intensity changed with the sphere diameters. This result showed the possibility of the selective measurement of the mitochondria density in the embryo in spite of the presence of the other cell organelle. We think that our light-scattering measurement system might be applicable to the evaluation method for the embryo viability.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsSteven L. Jacques
Pages298-302
Number of pages5
Publication statusPublished - 1996 Jan 1
EventLaser-Tissue Interaction VII - San Jose, CA, USA
Duration: 1996 Jan 291996 Feb 1

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume2681

Other

OtherLaser-Tissue Interaction VII
CitySan Jose, CA, USA
Period96/1/2996/2/1

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ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

Cite this

Itoh, H., Arai, T. M. D., & Kikuchi, M. (1996). Fiber optic light-scattering measurement system for evaluation of embryo viability: model experiment. In S. L. Jacques (Ed.), Proceedings of SPIE - The International Society for Optical Engineering (pp. 298-302). (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 2681).