Fiber optic monitoring system for mouse embryo evaluation

Harumi Itoh, Tsunenori Arai, Makoto Kikuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We constructed a fiber-optic long-term monitoring system for mouse embryo, which developed a new quantitative and non-invasive evaluation method for human embryo. We monitored the changes of light scattering from mouse embryo which might relate to the state of blastomere and change in intercellular microstructures. We employed a couple of optical fibers to measure the scattering. These fibers were arranged in a light angle to the optical axis of a microscope to obtain adaptability for various measurements. A He-Ne laser with 632.8 nm in wavelength was used as a light source. The emission fiber (56 μmΦ; core) and sensing fiber (400 μmΦ; core, NA = 0.43) were used. We observed the light scattering changes from the embryo, which were over 200 times higher than the noise level with 30 s in period. This result might be attributed to the structural and/or functional changes in the mouse embryo.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
PublisherSociety of Photo-Optical Instrumentation Engineers
Pages458-461
Number of pages4
Volume2388
ISBN (Print)0819417351
Publication statusPublished - 1995
Externally publishedYes
EventAdvances in Fluorescence Sensing Technology II - San Jose, CA, USA
Duration: 1995 Feb 61995 Feb 8

Other

OtherAdvances in Fluorescence Sensing Technology II
CitySan Jose, CA, USA
Period95/2/695/2/8

Fingerprint

embryos
Fiber optics
mice
fiber optics
Light scattering
Fibers
Monitoring
evaluation
fibers
light scattering
Light sources
Optical fibers
Microscopes
Scattering
Wavelength
Microstructure
Lasers
light sources
optical fibers
microscopes

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Itoh, H., Arai, T., & Kikuchi, M. (1995). Fiber optic monitoring system for mouse embryo evaluation. In Proceedings of SPIE - The International Society for Optical Engineering (Vol. 2388, pp. 458-461). Society of Photo-Optical Instrumentation Engineers.

Fiber optic monitoring system for mouse embryo evaluation. / Itoh, Harumi; Arai, Tsunenori; Kikuchi, Makoto.

Proceedings of SPIE - The International Society for Optical Engineering. Vol. 2388 Society of Photo-Optical Instrumentation Engineers, 1995. p. 458-461.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Itoh, H, Arai, T & Kikuchi, M 1995, Fiber optic monitoring system for mouse embryo evaluation. in Proceedings of SPIE - The International Society for Optical Engineering. vol. 2388, Society of Photo-Optical Instrumentation Engineers, pp. 458-461, Advances in Fluorescence Sensing Technology II, San Jose, CA, USA, 95/2/6.
Itoh H, Arai T, Kikuchi M. Fiber optic monitoring system for mouse embryo evaluation. In Proceedings of SPIE - The International Society for Optical Engineering. Vol. 2388. Society of Photo-Optical Instrumentation Engineers. 1995. p. 458-461
Itoh, Harumi ; Arai, Tsunenori ; Kikuchi, Makoto. / Fiber optic monitoring system for mouse embryo evaluation. Proceedings of SPIE - The International Society for Optical Engineering. Vol. 2388 Society of Photo-Optical Instrumentation Engineers, 1995. pp. 458-461
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