Fiber optic monitoring system for mouse embryo evaluation

Harumi Itoh, Tsunenori Arai, Makoto Kikuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We constructed a fiber-optic long-term monitoring system for mouse embryo, which developed a new quantitative and non-invasive evaluation method for human embryo. We monitored the changes of light scattering from mouse embryo which might relate to the state of blastomere and change in intercellular microstructures. We employed a couple of optical fibers to measure the scattering. These fibers were arranged in a light angle to the optical axis of a microscope to obtain adaptability for various measurements. A He-Ne laser with 632.8 nm in wavelength was used as a light source. The emission fiber (56 μmΦ; core) and sensing fiber (400 μmΦ; core, NA = 0.43) were used. We observed the light scattering changes from the embryo, which were over 200 times higher than the noise level with 30 s in period. This result might be attributed to the structural and/or functional changes in the mouse embryo.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
PublisherSociety of Photo-Optical Instrumentation Engineers
Pages458-461
Number of pages4
ISBN (Print)0819417351
Publication statusPublished - 1995 Jan 1
EventAdvances in Fluorescence Sensing Technology II - San Jose, CA, USA
Duration: 1995 Feb 61995 Feb 8

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume2388
ISSN (Print)0277-786X

Other

OtherAdvances in Fluorescence Sensing Technology II
CitySan Jose, CA, USA
Period95/2/695/2/8

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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  • Cite this

    Itoh, H., Arai, T., & Kikuchi, M. (1995). Fiber optic monitoring system for mouse embryo evaluation. In Proceedings of SPIE - The International Society for Optical Engineering (pp. 458-461). (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 2388). Society of Photo-Optical Instrumentation Engineers.