Fibrinogen-conjugated albumin polymers and their interaction with platelets under flow conditions

S. Takeoka, Y. Teramura, Y. Okamura, M. Handa, Y. Ikeda, E. Tsuchida

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Albumin polymers, having an average diameter of 1020 ± 250 nm, were prepared by the disulfide polymerization of recombinant human serum albumin (rHSA) by controlling of the pH and temperature. Fibrinogen could be conjugated on the surface of an albumin polymer using N-succinimidyl 3-(2-pyridyldithio)propionate (SPDP). Under flow conditions, the fibrinogen-conjugated albumin polymers (fibrinogen-albumin polymers) were irreversibly attached to the platelet-immobilized surface in the reconstituted blood at a low platelet concentration ([platelet] = 5.0 × 104/μL, a 5-fold diluted platelet concentration), and the attachment was suppressed by the addition of anti-GPIIb/IIIa monoclonal antibodies. It was confirmed that fibrinogen-albumin polymers specifically interacted with GPIIb/IIIa expressed on the surface of the activated platelets. Although platelets with a low platelet concentration were hardly attached to the platelet-immobilized surface under the flow conditions, the addition of fibrinogen-albumin polymers enhanced the attachment of the remaining platelets to the surface, indicating that the fibrinogen-albumin polymers would help the hemostatic ability of platelets at the site of vascular injury of patients in thrombocytopenia.

Original languageEnglish
Pages (from-to)1192-1197
Number of pages6
JournalBiomacromolecules
Volume2
Issue number4
DOIs
Publication statusPublished - 2001

Fingerprint

Conjugated polymers
Platelets
Fibrinogen
Albumins
Polymers
Blood Platelets
Monoclonal antibodies
Vascular System Injuries
Hemostatics
Serum Albumin
Polymerization
Disulfides
Thrombocytopenia
Blood
Monoclonal Antibodies
Temperature

ASJC Scopus subject areas

  • Organic Chemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Takeoka, S., Teramura, Y., Okamura, Y., Handa, M., Ikeda, Y., & Tsuchida, E. (2001). Fibrinogen-conjugated albumin polymers and their interaction with platelets under flow conditions. Biomacromolecules, 2(4), 1192-1197. https://doi.org/10.1021/bm015554o

Fibrinogen-conjugated albumin polymers and their interaction with platelets under flow conditions. / Takeoka, S.; Teramura, Y.; Okamura, Y.; Handa, M.; Ikeda, Y.; Tsuchida, E.

In: Biomacromolecules, Vol. 2, No. 4, 2001, p. 1192-1197.

Research output: Contribution to journalArticle

Takeoka, S, Teramura, Y, Okamura, Y, Handa, M, Ikeda, Y & Tsuchida, E 2001, 'Fibrinogen-conjugated albumin polymers and their interaction with platelets under flow conditions', Biomacromolecules, vol. 2, no. 4, pp. 1192-1197. https://doi.org/10.1021/bm015554o
Takeoka, S. ; Teramura, Y. ; Okamura, Y. ; Handa, M. ; Ikeda, Y. ; Tsuchida, E. / Fibrinogen-conjugated albumin polymers and their interaction with platelets under flow conditions. In: Biomacromolecules. 2001 ; Vol. 2, No. 4. pp. 1192-1197.
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