Fibroblast growth factor receptor-1 is expressed by endothelial progenitor cells

Patricia E. Burger, Sandra Coetzee, Wallace L. McKeehan, Mikio Kan, Perry Cook, Yong Fan, Toshio Suda, Robert P. Hebbel, Nicolas Novitzky, William A. Muller, E. Lynette Wilson

Research output: Contribution to journalArticle

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Abstract

Recent experiments show that hematopoietic progenitor cell populations contain endothelial precursor cells. We have isolated a population of CD34 + cells that expresses fibroblast growth factor receptor-1 (FGFR-1) and that differentiates into endothelial cells in vitro. We find that 4.5% ± 2.1% of CD34 + cells isolated from bone marrow, cord blood, and mobilized peripheral blood express FGFR-1 and that viable CD34 +FGFR + cells are small, with little granularity, and express both primitive hematopoietic and endothelial markers on their surface. The primitive hematopoietic markers AC133, c-kit, and Thy-1 are coexpressed by 75%, 85%, and 64% of CD34 +FGFR + cells, respectively. Most of the CD34 +FGFR + cells also express antigens found on endothelial cells, such as CD31, vascular endothelial growth factor receptor-2, and the endothelial-specific cell surface marker, vascular endothelial cadherin (VE-cadherin), whereas 56% to 60% of the cells express Tie, Tek, and the endothelial-specific marker, P1H12. The CD34 +FGFR + population is enriched in cells expressing endothelial-specific antigens compared with the CD34 + population. Isolated CD34 +FGFR + cells grow slowly in culture, are stimulated by fibroblast growth factor-2 and vascular endothelial growth factor, and give rise to cells that express von Willebrand factor and VE-cadherin and that incorporate acetylated low-density lipoprotein. These experiments show that FGFR-1 is expressed by a subpopulation of CD34 + cells that give rise to endothelial cells in vitro, indicating that this population contains endothelial stem/progenitor cells.

Original languageEnglish
Pages (from-to)3527-3535
Number of pages9
JournalBlood
Volume100
Issue number10
DOIs
Publication statusPublished - 2002 Nov 15

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Receptor, Fibroblast Growth Factor, Type 1
Endothelial cells
Endothelial Cells
Blood
Population
Antigens
Vascular Endothelial Growth Factor Receptor-2
von Willebrand Factor
Fibroblast Growth Factor 2
Stem cells
Vascular Endothelial Growth Factor A
Bone
Experiments
Cells
Endothelial Progenitor Cells
Hematopoietic Stem Cells
Fetal Blood
Bone Marrow Cells
Stem Cells

ASJC Scopus subject areas

  • Hematology

Cite this

Burger, P. E., Coetzee, S., McKeehan, W. L., Kan, M., Cook, P., Fan, Y., ... Lynette Wilson, E. (2002). Fibroblast growth factor receptor-1 is expressed by endothelial progenitor cells. Blood, 100(10), 3527-3535. https://doi.org/10.1182/blood.V100.10.3527

Fibroblast growth factor receptor-1 is expressed by endothelial progenitor cells. / Burger, Patricia E.; Coetzee, Sandra; McKeehan, Wallace L.; Kan, Mikio; Cook, Perry; Fan, Yong; Suda, Toshio; Hebbel, Robert P.; Novitzky, Nicolas; Muller, William A.; Lynette Wilson, E.

In: Blood, Vol. 100, No. 10, 15.11.2002, p. 3527-3535.

Research output: Contribution to journalArticle

Burger, PE, Coetzee, S, McKeehan, WL, Kan, M, Cook, P, Fan, Y, Suda, T, Hebbel, RP, Novitzky, N, Muller, WA & Lynette Wilson, E 2002, 'Fibroblast growth factor receptor-1 is expressed by endothelial progenitor cells', Blood, vol. 100, no. 10, pp. 3527-3535. https://doi.org/10.1182/blood.V100.10.3527
Burger PE, Coetzee S, McKeehan WL, Kan M, Cook P, Fan Y et al. Fibroblast growth factor receptor-1 is expressed by endothelial progenitor cells. Blood. 2002 Nov 15;100(10):3527-3535. https://doi.org/10.1182/blood.V100.10.3527
Burger, Patricia E. ; Coetzee, Sandra ; McKeehan, Wallace L. ; Kan, Mikio ; Cook, Perry ; Fan, Yong ; Suda, Toshio ; Hebbel, Robert P. ; Novitzky, Nicolas ; Muller, William A. ; Lynette Wilson, E. / Fibroblast growth factor receptor-1 is expressed by endothelial progenitor cells. In: Blood. 2002 ; Vol. 100, No. 10. pp. 3527-3535.
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